| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
We developed a high-efficient synthesis method of triazole-linked nucleic acid using click chemistry for nucleic acid elongation reaction and realized its functional development in life science. In searching for a click-linking method between RNA and amino acid planned at the beginning, azidation to the DNA end and the click-linking method were developed. These methods enabled us to realize a convenient double-stranded DNA template preparation for next-generation DNA sequencers. In addition, we switched the research target from modified tRNA to more challenging modified mRNA without any examples. We realized modified mRNA with a non-phosphate-linked codon for the first time. We also clarified the effect of the triazole linkages in the double-strand formation of such modified RNA from the thermodynamic analysis.
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