| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
The purpose of this study was to develop of programmed self-assembly technology of protein nanobuilding blocks by thermal control. We developed a hyperstable mutant Super WA20 (SUWA) of a de novo protein WA20. The SUWA protein denatured with an extremely high midpoint temperature (Tm = 122°C), much higher than the Tm of WA20 (75°C). We constructed protein nanobuilding blocks which can be reversibly denatured and reconstituted by temperature control. For example, we developed a protein nanobuilding block, SUWA-foldon, which can be reversibly denatured and reconstituted by programmed temperature changes to control the reconstruction of hexamer or dodecamer complexes.
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