| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Nitric oxide (NO) functions as a signal molecule in our bodies and has been well studied as vasodilators and regulator of immune systems. However, its precise roles in neuronal system has not been clarified. This research aimed for elucidating the mechanism of nitric oxide production and its downstream roles by utilizing novel material that can release NO by light irradiation. By utilizing lasers for the trigger of NO release, the spatial and temporal resolution could be precisely controlled. Using this material, the effect of NO to neuronal development was investigated in PC 12 cells. The specific exposure to NO resulted in the shrinkage of neuronal axons.
On the other hand, a physical association between an NO-responding Ca2+ channel and nitric oxide synthase via an adaptor protein was discovered. This interaction could be a mechanism to efficiently amplify the Ca2+ and NO production for a positive feedback of the signals.
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