| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Chemical synthesis is an indispensable technique for the preparation of unique proteins, which cannot be prepared by recombinant technique. In this work, we have developed a new regioselective peptide coupling strategy that enables one-pot peptide ligations. This new technique is based on the unique switchable reactivity of peptide-guanidine, an emerging peptide derivative. As a result, we succeeded in the assembly of full legth of erythropoieitn consisting of 166 amino acids from 4 peptide segments in one-pot manner. Furthermore, we also successfully synthesized a small circular protein by using th new regioselective peptide coupling strategy.
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