| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
We rationally constructed three types of energy-saving, eukaryotic riboswitches (ligand-responsive regulatory RNAs) that function in eukaryotic translation systems. The first one upregulates translation mediated by a 3' cap-independent translation element (3' CITE) in response to its specific ligand. The second one ligand-dependently downregulates translation mediated by an internal ribosome entry site (IRES). The last one ligand-responsively downregulates canonical translation. Because all of them require no major hybridization switches for gene regulation, they require less energy for their conformational changes, thus showing higher switching efficiencies. In addition, their ligand-specificity can easily be changed just by replacing a part of them (specifically, the aptamer domain) to obtain a riboswitch responsive to a user-defined ligand.
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