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Development of gold nanorod synthesis directed by peptide self-assembled nanostructures

Research Project

Project/Area Number 16K05854
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Bio-related chemistry
Research InstitutionRyukoku University

Principal Investigator

Tomizaki Kin-ya  龍谷大学, 理工学部, 教授 (90397026)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords金ナノロッド / ペプチド / フォトサーマル療法 / 自己集合化 / ペプチド集合体 / 金ナノ結晶 / N-メチルアミノ酸 / 金ナノ粒子 / 生体機能化学
Outline of Final Research Achievements

Gold nanorods are potential for absorbing near-IR light of a specific wavelength derived from surface plasmon resonance and efficiently converting light into heat. Thus, it is promised to apply to photothermal cancer treatment. Curremtly, CTAB (cetyltrimethylammonium bromide) is used as a template molecule in the conventional synthetic methods of gold nanorods. However, CTAB itself is cytotoxic, it is necessary to remove CTAB for biological use. In this study, peptides having a fatty acid mimicking CTAB were synthesized (C11-R : undecanoyl-Arg-NH2 and C8-GR : octanoyl-Gly-Arg-NH2), and employed to synthesize gold nanorods. It was found that gold nanorods were synthesized by mixing the peptides and CTAB at ratios of 1%, 5%, and 10% peptide to CTAB. With increasing peptide concentration, the aspect ratio of the gold nano rods decreased. These results suggest that the 10% of CTAB could be replaced with such peptides in the present method and contribute to reduce cytotoxicity.

Academic Significance and Societal Importance of the Research Achievements

本研究成果は、我々の体内にも存在するペプチドを用いて金ナノロッドの合成を行い、得られた金ナノロッドが示す近赤外光を熱に変換する特性を利用するガン治療法の拡大へと繋ぐことができるものである。具体的には、①金ナノ結晶合成において、特殊な製造装置を必要とせず、低エネルギー、低毒性にて試薬合成が可能となる、②皮膚透過性の高い近赤外光を利用することで、皮膚組織近傍のガン治療等におけるフォトサーマル療法への応用が期待できる、③生物が行うバイオミネラリゼーションメカニズム解明のための生物学的モデル構築を行うことができる、に大きく寄与する。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (17 results)

All 2018 2017 2016

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (13 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Modification of the N-Terminus of a Calcium Carbonate Precipitating Peptide Affects Calcium Carbonate Mineralization2018

    • Author(s)
      Usui, K.; Yokota, S.-I.; Ozaki, M.; Sakashita S.; Imai, T.; Tomizaki, K.-Y.
    • Journal Title

      Protein & Peptide Letters

      Volume: 25 Issue: 1 Pages: 42-47

    • DOI

      10.2174/0929866525666171221114658

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] α-Helical Peptide-Gold Nanoparticle Hybrids: Synthesis, Characterization, and Catalytic Activity2018

    • Author(s)
      Tomizaki KY, Yamaguchi Y, Tsukamoto N, Imai T.
    • Journal Title

      Protein Pept Lett.

      Volume: 25(1) Issue: 1 Pages: 56-63

    • DOI

      10.2174/0929866525666171214113324

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Incorporation of an N-methyl Amino Acid into a Template Peptide Alters Anisotropy in the Crystal Growth of Gold Nanoparticles Synthesized by the Peptide Template Method2018

    • Author(s)
      Tomizaki KY, Nanbu R, Kasuga M, Imai T.
    • Journal Title

      Protein Pept Lett.

      Volume: 25(1) Issue: 1 Pages: 48-55

    • DOI

      10.2174/0929866525666171214105611

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Non-Covalent Loading of Anti-Cancer Doxorubicin by Modularizable Peptide Self-Assemblies for a Nanoscale Drug Carrier2017

    • Author(s)
      Tomizaki KY, Kishioka K, Kataoka S, Miyatani M, Ikeda T, Komada M, Imai T, Usui K.
    • Journal Title

      Molecules

      Volume: 22(11) Issue: 11 Pages: 1916-1916

    • DOI

      10.3390/molecules22111916

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] 脂肪鎖含有ペプチドの合成と金ナノ粒子合成におけるペプチド濃度の影響2018

    • Author(s)
      塚本直幸・今井崇人・富崎欣也
    • Organizer
      第12回バイオ関連シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] Synthesis of Fatty Acid-Containing Peptides and the Effect of Peptide Concentration in Template-Directed Gold Nanocrystal Synthesis2018

    • Author(s)
      Tsukamoto, N.; Imai, T.; Tomizaki, K.-Y.
    • Organizer
      The 10th International Peptide Symposium/ The 55th Japanese Peptide Symposium
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effect of surfactant-like peptides on gold nanoparticle synthesis2018

    • Author(s)
      Tsukamoto, N.; Imai, T.; Tomizaki, K.-Y.
    • Organizer
      日本化学会第98春季年会
    • Related Report
      2017 Research-status Report
  • [Presentation] Minimization of peptides that selectively reduce gold ions in the presence of different noble metal ions2018

    • Author(s)
      Tonoda, T.; Imai, T.; Asano, M.; Tomizaki, K.-Y.
    • Organizer
      日本化学会第98春季年会
    • Related Report
      2017 Research-status Report
  • [Presentation] Effect of light irradiation on mineralization of noble metal ions by aromatic side chain-containing peptides2018

    • Author(s)
      Uchiyama, T.; Imai, T.; Asano, M.; Tomizaki, K.-Y.
    • Organizer
      日本化学会第98春季年会
    • Related Report
      2017 Research-status Report
  • [Presentation] 種々の芳香環側鎖を含有するペプチドを用いた光照射下における貴金属粒子の合成2017

    • Author(s)
      内山隆博・今井崇人・富崎欣也
    • Organizer
      第11回バイオ関連化学シンポジウム
    • Related Report
      2017 Research-status Report
  • [Presentation] 脂肪鎖含有ペプチドの合成と金ナノ粒子合成における鋳型効果2017

    • Author(s)
      塚本直幸・今井崇人・富崎欣也
    • Organizer
      第11回バイオ関連化学シンポジウム
    • Related Report
      2017 Research-status Report
  • [Presentation] Synthesis of Noble Metal Nanoparticles by Peptides Containing Various Aromatic Side Chains under Light Irradiation Conditions2017

    • Author(s)
      Uchiyama, T.; Imai, T.; Tomizaki, K.-Y.
    • Organizer
      The 54th Japanese Peptide Symposium
    • Related Report
      2017 Research-status Report
  • [Presentation] Synthesis of Fatty Acid Containing Peptides and Their Use for Gold Nanocrystal Synthesis as Templates2017

    • Author(s)
      Tsukamoto, N.; Imai, T.; Tomizaki, K.-Y.
    • Organizer
      The 54th Japanese Peptide Symposium
    • Related Report
      2017 Research-status Report
  • [Presentation] Selective Precipitation of Gold Nanocrystals from Aqueous Solution Containing Platinum Ion Contaminants by Peptides2017

    • Author(s)
      Tomizaki, K.-Y.; Okamoto, T.; Imai, T.; Asano, M.
    • Organizer
      The 25th American Peptide Symposium
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Anisotropic Growth of Gold Nanocrystals Along the Interior Cavity of a Peptide Self-Assembly2016

    • Author(s)
      Kin-ya Tomizaki
    • Organizer
      The 53rd Japanese Peptide Symposium
    • Place of Presentation
      京都TERRSA
    • Year and Date
      2016-10-26
    • Related Report
      2016 Research-status Report
  • [Presentation] 界面活性剤様ペプチドの自己集合化と金ナノ粒子合成における鋳型効果2016

    • Author(s)
      山田直輝(学生)
    • Organizer
      第10回バイオ関連化学シンポジウム
    • Place of Presentation
      石川県立音楽堂
    • Year and Date
      2016-09-07
    • Related Report
      2016 Research-status Report
  • [Presentation] Synthesis of Anisotropic Gold Nanocrystals within the Cavity of a Self-Assembled Peptide Nanoarchitectur2016

    • Author(s)
      Kin-ya Tomizaki
    • Organizer
      The 34th European Peptide Symposium-The 8th International Peptide Symposium
    • Place of Presentation
      Leipzig, Germany
    • Year and Date
      2016-09-04
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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