| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
The translation factor EF-P is a bacterial L-shaped protein that alleviates the ribosome stalling that occurs at consequtive prolines in the genetic code translation. In N. meningitidis EF-P, rhamnosylation of the arginine residue at the L-shaped tip is very important for its activity. We solved the crystal structure of the rhamnosylation enzyme EarP and the EarP / EF-P complex from N. meningitidis, and identified amino acid residues important for Arg32 rhamnosylation activity. The conformation of the rhamnose moiety is suitable for the reaction was estimated by the docking simulation, and the reaction mechanism of EF-P(Arg32) rhamnosylation was proposed.
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