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Characterization of biodegradable nanofibers for cryopreservation

Research Project

Project/Area Number 16K05908
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Polymer/Textile materials
Research InstitutionUniversity of Fukui

Principal Investigator

FUJITA SATOSHI  福井大学, 学術研究院工学系部門, 准教授 (60504652)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsナノファイバー / 生体分解性 / 細胞凍結 / 細胞接着 / エレクトロスピニング / スキャフォールド / PHBH / 生体吸収性 / 凍結保存 / 高分子構造 / 生体材料 / 細胞・組織 / 高分子構造・物性 / 再生医学
Outline of Final Research Achievements

This study is focused on the application to culture scaffolds for cell cryopreservation by using poly (3-(R)-hydroxybutyric acid-3-(R)-hydroxyhexanoic acid) (PHBH) nanofibers. As a result of the investigation of the physical properties and biocompatibility of PHBH, the following results were obtained. (1) Regarding crystal structure and thermal properties, PLLA showed high crystallinity in nanofibers but low in films, whereas PHBH showed no significant difference between nanofibers and films There was no remarkable difference in the crystal structure of. (2) The inflammation after the implantation of PHBH nanofibers was lower than PLLA. In addition, the mechanism of degradation mode in the physiological condition was proposed. (3) The cells adhered to the surface of PHBH nanofibers were less likely to exfoliate than PLLA and maintained the extended shape even in low temperature. It was available as a cell scaffold material that can be directly frozen with PHBH nanofibers attached.

Academic Significance and Societal Importance of the Research Achievements

PHBHの生分解性挙動から,埋込材料として有効であることが示された。PHBH基材は、冷却および凍結後も細胞接着を維持できる材料であることも示された。さらに凍結融解によって低い細胞生存率、接着細胞数を示したPLLAおよびPS基材は、低温で収縮した細胞の接着が弱くなり、凍結融解によって細胞が剥離、収縮による物理的ストレスがかかるメカニズムが示された。PHBHは微生物由来の天然ポリマーであることから,PLLA等の合成ポリマーに代わる有望な材料のひとつとして,本研究での成果を軸に今後の医療応用や産業利用が期待される。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (17 results)

All 2018 2017 2016 Other

All Journal Article (4 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (12 results) (of which Int'l Joint Research: 4 results,  Invited: 1 results) Remarks (1 results)

  • [Journal Article] Native collagen hydrogel nanofibres with anisotropic structure using core-shell electrospinning2018

    • Author(s)
      Wakuda Yuka、Nishimoto Shohei、Suye Shin-ichiro、Fujita Satoshi
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1

    • DOI

      10.1038/s41598-018-24700-9

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Direct cryopreservation of adherent cells on an elastic nanofiber sheet featuring a low glass-transition temperature2017

    • Author(s)
      Batnyam Onon、Suye Shin-ichiro、Fujita Satoshi
    • Journal Title

      RSC Advances

      Volume: 7 Issue: 81 Pages: 51264-51271

    • DOI

      10.1039/c7ra10604a

    • NAID

      120006478473

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Estimation of the Core-Shell Formation Efficiency of Electrospun Collagen/Polylactic Acid Nanofibers2016

    • Author(s)
      加藤 新, 末 信一朗, 藤田 聡
    • Journal Title

      KOBUNSHI RONBUNSHU

      Volume: 73 Issue: 4 Pages: 366-369

    • DOI

      10.1295/koron.2015-0086

    • NAID

      130005165605

    • ISSN
      0386-2186, 1881-5685
    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Design and Fabrication of Conductive Nanofiber Using Electrospinning2016

    • Author(s)
      坂元 博昭, 藤田 聡, 末 信一朗
    • Journal Title

      IEEJ Transactions on Sensors and Micromachines

      Volume: 136 Issue: 11 Pages: 461-464

    • DOI

      10.1541/ieejsmas.136.461

    • NAID

      130005268279

    • ISSN
      1341-8939, 1347-5525
    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] Electrospun Co-axial Nanofibers as DDS Matrix for Tunable Release2018

    • Author(s)
      S. Fujita
    • Organizer
      Taiwan-Japan- Korea Trilateral Conference on Nanomedicine, 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] PLLA-based core-shell nanofibers for long-term drug release2018

    • Author(s)
      T. Hibino, S. Suye, S. Fujita
    • Organizer
      5th TERMIS World Congress
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 生分解性ナノファイバーからの薬剤放出挙動の解析2018

    • Author(s)
      日比野隼也, 末信一朗, 藤田聡
    • Organizer
      平成30年度高分子学会北陸支部研究発表会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ナノファイバー基材上での接着細胞の直接凍結保存の影響の解析2018

    • Author(s)
      丸山拓人, 末信一朗, 藤田聡
    • Organizer
      平成30年度繊維学会秋季研究発表会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 芯鞘ナノファイバーを用いた薬剤徐放の時間的制御2018

    • Author(s)
      日比野隼也, 末信一朗, 藤田聡
    • Organizer
      平成30年度繊維学会秋季研究発表会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 長期薬剤放出制御を目的とした担持ナノファイバーの創製2018

    • Author(s)
      日比野 隼也, 末 信一朗, 藤田 聡
    • Organizer
      第67回高分子学会年次大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ナノファイバーシートを用いた接着細胞の直接凍結による細胞培養の高効率化2017

    • Author(s)
      丸山 拓人, 小暮 麻心, 末 信一朗, 藤田 聡
    • Organizer
      日本バイオマテリアル学会第6回北信越研究発表会
    • Related Report
      2017 Research-status Report
  • [Presentation] ポリ(3-(R)-ヒドロキシ酪酸-3-(R)-ヒドロキシヘキサン酸)(PHBH)の生体吸収性の評価2016

    • Author(s)
      丸淵 那々, 中路 正, 北野 博巳, 藤田 聡, 末 信一朗
    • Organizer
      日本バイオマテリアル学会第5回北陸信越若手研究発表会
    • Place of Presentation
      長岡
    • Year and Date
      2016-12-09
    • Related Report
      2016 Research-status Report
  • [Presentation] 薬剤徐放のためのコラーゲン/PLLA芯鞘ナノファイバーの創成と機能評価2016

    • Author(s)
      日比野 隼也, 末 信一朗, 藤田 聡
    • Organizer
      日本バイオマテリアル学会第5回北陸信越若手研究発表会
    • Place of Presentation
      長岡
    • Year and Date
      2016-12-09
    • Related Report
      2016 Research-status Report
  • [Presentation] 薬剤徐放のためのコラーゲン/PLLA芯鞘ナノファイバーの創成と機能評価2016

    • Author(s)
      日比野 隼也, 末 信一朗, 藤田 聡
    • Organizer
      平成28年度高分子学会北陸地区若手研究会
    • Place of Presentation
      福井
    • Year and Date
      2016-11-11
    • Related Report
      2016 Research-status Report
  • [Presentation] Fabrication of alginate gel fibers by co-axial electrospinning and application for cell culture scaffold2016

    • Author(s)
      Y. Wakuda, S. Suye, S. Fujita
    • Organizer
      9th International Conference on Fiber and Polymer Biotechnology
    • Place of Presentation
      Osaka
    • Year and Date
      2016-09-07
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Degradation and inflammatory response of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) under physiological conditions2016

    • Author(s)
      S. Fujita, N. Marubuchi, T. Nakaji-Hirabayashi, Y. Kimura, S. Furukawa, H. Kitano, S. Suye
    • Organizer
      10th World Biomaterials Congress
    • Place of Presentation
      Montreal
    • Year and Date
      2016-05-17
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Remarks] 福井大学工学研究科 生命機能工学研究室

    • URL

      http://acbio2.acbio.u-fukui.ac.jp/bioeng/suye/

    • Related Report
      2016 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2021-01-27  

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