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3-D Measurement of the Blood Flow in the Heart of Medaka Embryo as Human Disease Model

Research Project

Project/Area Number 16K06107
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Thermal engineering
Research InstitutionUtsunomiya University

Principal Investigator

Ninomiya Nao  宇都宮大学, 工学部, 准教授 (70212123)

Co-Investigator(Kenkyū-buntansha) 松田 勝  宇都宮大学, バイオサイエンス教育研究センター, 教授 (20414013)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords三次元計測 / PIV / ヒト疾患モデル / 血流 / メダカ胚 / in-vivo / デジタルホログラフィー / 三次元速度計測 / 応用光学 / 流体力学 / 発生 / ホログラフィー
Outline of Final Research Achievements

The results of this study can be classified in to three parts.
First, the three-dimensional velocity measurement by the Doppler-phase-shifting holography has been developed. It can measure the three-dimensional velocity by single camera and the expanding optics which is necessary for the microscopic observation has also been utilized. Second, the in-vivo velocity measurement in the heart of Medaka embryo has been achieved by using Particle Image Velocimetry. Third, the special optical prism which enables the stereoscopic measurement under microscope has been designed.

Academic Significance and Societal Importance of the Research Achievements

本研究は、in-vivoにて生体の心臓内血流をPIVにより実測した初めて研究成果であり、生物学的にも工学的に非常に有意義な研究である。本研究では、血流の二次元計測までしか行えなかったが、単眼観察しか出来ないという顕微鏡下で三次元計測を可能とする二種類の計測方法の提案も行われており、将来的に生体内血流の三次元計測の可能性を示した。
また、遺伝子操作し、人工的に循環器系異常持たせたゼブラフィッシュの血流計測を行い、遺伝的心疾患の早期発見の可能性を示した。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (55 results)

All 2019 2018 2017 2016

All Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 7 results,  Acknowledgement Compliant: 1 results) Presentation (45 results) (of which Int'l Joint Research: 13 results,  Invited: 1 results) Book (3 results)

  • [Journal Article] Video-rate quantitative phase analysis by a DIC microscope using a polarization camera2019

    • Author(s)
      Shibata Shuhei、Takano Wataru、Hagen Nathan、Matsuda Masaru、Otani Yukitoshi
    • Journal Title

      Biomedical Optics Express

      Volume: 10 Issue: 3 Pages: 1273-1273

    • DOI

      10.1364/boe.10.001273

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural investigation of α-l-fucosidase from the pancreas of Patiria pectinifera, based on molecular cloning2019

    • Author(s)
      Ono Akiko、Suzuki Tomohiro、Gotoh Saki、Kono Haruka、Matsui Megumi、Aoki Daichi、Matsuda Masaru、Kawagishi Hirokazu、Ogata Makoto
    • Journal Title

      Carbohydrate Research

      Volume: 475 Pages: 27-33

    • DOI

      10.1016/j.carres.2019.02.001

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Interaction between two falling droplets in the liquid?liquid two-phase flow2018

    • Author(s)
      Ninomiya Nao、Tsukada Shogo、Ikeda Motohiro、Doh Deog-Hee
    • Journal Title

      Journal of Visualization

      Volume: 21 Issue: 6 Pages: 1009-1016

    • DOI

      10.1007/s12650-018-0509-z

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] 3-D measurement of 2-D jet by 3-D 3-C SPIV2018

    • Author(s)
      Ninomiya Nao、Tanaka Yukihisa、Sotome Satoshi、Eda Masahide、Watanabe Atsushi
    • Journal Title

      Journal of Visualization

      Volume: 22 Issue: 2 Pages: 305-312

    • DOI

      10.1007/s12650-018-0529-8

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] シイタケの子実体形成過程における水分経路の蛍光色素による可視化2018

    • Author(s)
      窪田敏和, 鈴木智大, 金野尚武, 二宮 尚
    • Journal Title

      日本きのこ学会誌

      Volume: 4 Pages: 156-160

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Origin of the widely and discontinuously distributed mitochondrial genotypes of Oryzias latipes: introduced or native genotypes?2018

    • Author(s)
      Iguchi Yuka、Nakao Ryohei、Matsuda Masaru、Takata Keisuke、Kitagawa Tadao
    • Journal Title

      Ichthyological Research

      Volume: 66 Issue: 1 Pages: 183-188

    • DOI

      10.1007/s10228-018-0662-3

    • NAID

      40021806385

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Three-Dimensional Velocity Measurement by a Single Camera using Doppler Phase-Shifting Holography2016

    • Author(s)
      N. Ninomiya, Y. Kubo, D. Barada and T. Kiire
    • Journal Title

      Measurement Science and Technology

      Volume: 27 Issue: 10 Pages: 104004-104004

    • DOI

      10.1088/0957-0233/27/10/104004

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] PIV Measurement of the Blood Flow in the Heart of Medaka Embryo2019

    • Author(s)
      N. Araki, N. Ninomiya and M. Matsuda
    • Organizer
      ASME-JSME-KSME Joint Fluid Eng. Conf. 2019
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Investigation on Energy Shares and Flow Structures of the Mixing Flows using 3D-POD Analyses2019

    • Author(s)
      D. Kong, G. Cho, N. Ninomiya, S. Kang and D.-H. Doh
    • Organizer
      ASME-JSME-KSME Joint Fluid Eng. Conf. 2019
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] In-vivo Measurement of the Blood Flow in the Heart of Medaka Embryo2019

    • Author(s)
      N. Ninomiya, M. Matsuda and N. Araki
    • Organizer
      13th Int’l Symp. on Particle Image Velocimetry
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] In-vivo PIV Measurement of the Blood Flow in the Heart of Medaka Embryo2019

    • Author(s)
      N. Ninomiya, M. Matsuda and N. Araki
    • Organizer
      2nd Pacific Rim Thermal Eng. Conf.
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ジーンボディにおけるDNAメチル化の機能的役割2019

    • Author(s)
      岩波礼将, 横井勇人, 秋山真太郎, 重水大智, 荒木夏生, 二宮 尚, 松田 勝, 鈴木徹, 下田修義
    • Organizer
      第13回日本エピジェネティクス研究会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] PIV Measurement of the Blood Flow of Medaka Embryo2018

    • Author(s)
      N. Ninomiya, M. Matsuda, T. Nako and N. Araki
    • Organizer
      18th Int'l Symp. on Flow Visualization
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 3D Velocity Measurement of Particles Moving with Different Speeds using Doppler Phase-Shifting Holo- graphy2018

    • Author(s)
      N. Ninomiya, M. Nishimura, I. Miyauchi and D. Barada
    • Organizer
      19th Int'l Symp. on Appl. Laser Imaging Tech. Fluid Mech.
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] メダカ胚の心臓及び大静脈内血流のPIV計測に関する研究2018

    • Author(s)
      荒木 夏生, 名古 達, 二宮 尚, 松田 勝
    • Organizer
      第55回日本伝熱シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] DPSHによる顕微鏡下での微粒子の三次元速度計測に関する研究2018

    • Author(s)
      吉田 亮太, 宮内 郁海, 西村 政彦, 二宮 尚
    • Organizer
      第55回日本伝熱シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] 二次元衝突噴流の自励振動2018

    • Author(s)
      魚谷 秀也, 二宮 尚
    • Organizer
      第46可視化情報シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] メダカ胚の心臓内血流のPIV計測2018

    • Author(s)
      荒木夏生, 二宮 尚, 松田 勝
    • Organizer
      第46可視化情報シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] キノコの成長過程における水分経路の可視化2018

    • Author(s)
      窪田 敏和, 二宮 尚, 金野 尚武, 鈴木 智大
    • Organizer
      第46可視化情報シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] メダカ属魚類の性決定遺伝子2018

    • Author(s)
      松田勝
    • Organizer
      日本水産学会平成30年秋季大会シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] メダカ胚の心臓血流のin-vivo計測2018

    • Author(s)
      荒木 夏生, 二宮 尚, 松田 勝
    • Organizer
      日本機械学会熱工学コンファレンス2018
    • Related Report
      2018 Annual Research Report
  • [Presentation] LIFによるキノコの形成過程における水分経路の可視化2018

    • Author(s)
      窪田 敏和, 二宮 尚, 金野 尚武, 鈴木 智大
    • Organizer
      日本機械学会熱工学コンファレンス2018
    • Related Report
      2018 Annual Research Report
  • [Presentation] PIV Measurement of the Blood Flow of Medaka Embryo2018

    • Author(s)
      N. Ninomiya, M. Matsuda, T. Nako and N. Araki
    • Organizer
      18th Int’l Symp. on Flow Visualization
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 3D Velocity Measurement of Particles Moving with Different Speeds using Doppler Phase-Shifting Holography2018

    • Author(s)
      N. Ninomiya, M. Nishimura, I. Miyauchi and D. Barada
    • Organizer
      19th Int’l Symp. on Appl. Laser Tech. Fluid Mech.
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] メダカ胚の心臓及び大静脈内血流のPIV計測に関する研究2018

    • Author(s)
      荒木夏生, 名古 達, 二宮 尚, 松田 勝
    • Organizer
      第55回日本伝熱シンポジウム
    • Related Report
      2017 Research-status Report
  • [Presentation] DPSHによる顕微鏡下での微粒子の三次元速度計測に関する研究2018

    • Author(s)
      吉田亮太, 宮内郁海, 西村政彦, 二宮 尚
    • Organizer
      第55回日本伝熱シンポジウム
    • Related Report
      2017 Research-status Report
  • [Presentation] 二次元衝突噴流の自励振動2018

    • Author(s)
      魚谷 秀也, 二宮 尚
    • Organizer
      第46回可視化情報シンポジウム
    • Related Report
      2017 Research-status Report
  • [Presentation] メダカ胚の心臓内血流のPIV計測2018

    • Author(s)
      荒木夏生, 二宮 尚, 松田 勝
    • Organizer
      第46回可視化情報シンポジウム
    • Related Report
      2017 Research-status Report
  • [Presentation] キノコの成長過程における水分経路の可視化2018

    • Author(s)
      窪田 敏和, 二宮 尚, 金野 尚武, 鈴木 智大
    • Organizer
      第46回可視化情報シンポジウム
    • Related Report
      2017 Research-status Report
  • [Presentation] 3-D 3-C PIVの高精度化と乱流計測2017

    • Author(s)
      田中 志尚, 二宮 尚
    • Organizer
      第54回日本伝熱シンポジウム
    • Place of Presentation
      大宮ソニックシティー, 大宮
    • Year and Date
      2017-05-24
    • Related Report
      2016 Research-status Report
  • [Presentation] 固体表面に衝突する液滴の飛散に及ぼす表面性状の影響2017

    • Author(s)
      加藤 大貴, 二宮 尚
    • Organizer
      第54回日本伝熱シンポジウム
    • Place of Presentation
      大宮ソニックシティー, 大宮
    • Year and Date
      2017-05-24
    • Related Report
      2016 Research-status Report
  • [Presentation] Collision Behavior of a Water Drop against the Solid Surface2017

    • Author(s)
      D. Kato and N. Ninomiya
    • Organizer
      9th JSME-KSME Thermal and Fluids Engineering Conference
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Accuracy Improvement of Turbulence Measurement by 3-D 3-C PIV2017

    • Author(s)
      Y. Tanaka and N. Ninomiya
    • Organizer
      11th Pacific Symp. on Flow Visualization and Image Processing
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] メダカ胚の心臓及び大静脈内血流のPIV計測に関する研究2017

    • Author(s)
      荒木夏生, 二宮 尚, 松田 勝
    • Organizer
      第45回可視化情報シンポジウム
    • Related Report
      2017 Research-status Report
  • [Presentation] 二次元衝突噴流の自励振動に関する研究2017

    • Author(s)
      魚谷秀也, 二宮 尚
    • Organizer
      第45回可視化情報シンポジウム
    • Related Report
      2017 Research-status Report
  • [Presentation] キノコの子実体形成過程における水分経路の可視化に関する研究2017

    • Author(s)
      窪田敏和, 二宮 尚
    • Organizer
      第45回可視化情報シンポジウム
    • Related Report
      2017 Research-status Report
  • [Presentation] PIV Measurement of the Blood Flow in the Main Vein of Medaka Embryo2017

    • Author(s)
      Toru Nako, Nao Ninomiya and Masaru Matsuda
    • Organizer
      The 23rd Japanese Medaka and Zebrafish meeting
    • Related Report
      2017 Research-status Report
  • [Presentation] PIV Measurement of the Blood Flow in the Heart of Medaka Embryo2017

    • Author(s)
      Natsuo Araki, Nao Ninomiya and Masaru Matsuda
    • Organizer
      The 23rd Japanese Medaka and Zebrafish meeting
    • Related Report
      2017 Research-status Report
  • [Presentation] 二次元衝突噴流の可視化とPIV計測2017

    • Author(s)
      魚谷 秀也, 二宮 尚
    • Organizer
      可視化情報全国講演会(室蘭2017)
    • Related Report
      2017 Research-status Report
  • [Presentation] キノコの子実体形成過程における水分経路の可視化2017

    • Author(s)
      窪田 敏和, 二宮 尚, 金野 尚武, 鈴木 智大
    • Organizer
      可視化情報全国講演会(室蘭2017)
    • Related Report
      2017 Research-status Report
  • [Presentation] Three-Dimensional Velocity Measurement of Particles by Doppler Phase-Shifting Holography2016

    • Author(s)
      T. Kindaichi and N. Ninomiya
    • Organizer
      4th International Forum on Heat Transfer
    • Place of Presentation
      Sendai, Japan
    • Year and Date
      2016-11-04
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] メダカ胚における大静脈内血流のPIV計測2016

    • Author(s)
      名古 達, 二宮 尚, 松田 勝
    • Organizer
      日本機械学会熱工学コンファレンス2016
    • Place of Presentation
      愛媛大学, 松山
    • Year and Date
      2016-10-22
    • Related Report
      2016 Research-status Report
  • [Presentation] 液滴の固体表面への衝突における表面性状の影響の可視化2016

    • Author(s)
      加藤 大貴, 二宮 尚
    • Organizer
      可視化情報全国講演会(日立2016)
    • Place of Presentation
      茨城大学, 日立
    • Year and Date
      2016-10-08
    • Related Report
      2016 Research-status Report
  • [Presentation] 3-D 3-C PIVの高精度化に関する研究2016

    • Author(s)
      田中 志尚, 二宮 尚
    • Organizer
      可視化情報全国講演会(日立2016)
    • Place of Presentation
      茨城大学, 日立
    • Year and Date
      2016-10-08
    • Related Report
      2016 Research-status Report
  • [Presentation] 自由落下する液滴の表面に対する衝突挙動に関する研究2016

    • Author(s)
      加藤 大貴, 二宮 尚
    • Organizer
      第44回可視化情報シンポジウム
    • Place of Presentation
      工学院大学, 東京
    • Year and Date
      2016-07-19
    • Related Report
      2016 Research-status Report
  • [Presentation] 3-D 3-C PIVの精度向上に関する研究2016

    • Author(s)
      田中 志尚, 二宮 尚
    • Organizer
      第44回可視化情報シンポジウム
    • Place of Presentation
      工学院大学, 東京
    • Year and Date
      2016-07-19
    • Related Report
      2016 Research-status Report
  • [Presentation] メダカ胚における心臓内血流のPIV計測に関する研究2016

    • Author(s)
      名古 達, 二宮 尚, 松田 勝
    • Organizer
      第44回可視化情報シンポジウム
    • Place of Presentation
      工学院大学, 東京
    • Year and Date
      2016-07-19
    • Related Report
      2016 Research-status Report
  • [Presentation] ドップラー位相シフトホログラフィを用いた微粒子の三次元速度計測に関する研究2016

    • Author(s)
      西村 政彦, 二宮 尚
    • Organizer
      第44回可視化情報シンポジウム
    • Place of Presentation
      工学院大学, 東京
    • Year and Date
      2016-07-19
    • Related Report
      2016 Research-status Report
  • [Presentation] 拡大光学系を用いたドップラー位相シフトホログラフィによる三次元計測に関する研究2016

    • Author(s)
      宮内 郁海, 二宮 尚
    • Organizer
      第44回可視化情報シンポジウム
    • Place of Presentation
      工学院大学, 東京
    • Year and Date
      2016-07-19
    • Related Report
      2016 Research-status Report
  • [Presentation] Three-Dimensional Velocity Measurement by a Single Camera Observation using Digital Holography2016

    • Author(s)
      N. Ninomiya
    • Organizer
      17th International Symposium on Flow Visualization
    • Place of Presentation
      Gatlinburg, Tennessee, USA
    • Year and Date
      2016-06-19
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 3-D Velocity Measurement of Small Particles by Doppler Phase-Shifting Holography2016

    • Author(s)
      N. Ninomiya, T. Kindaichi, T. Ouchi, and D. Barada
    • Organizer
      17th International Symposium on Flow Visualization
    • Place of Presentation
      Gatlinburg, Tennessee, USA
    • Year and Date
      2016-06-19
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] ドップラー位相シフトホログラフィによる微粒子の三次元速度計測2016

    • Author(s)
      金田一哲平, 二宮 尚
    • Organizer
      第53回日本伝熱シンポジウム
    • Place of Presentation
      大阪府立国際会議場, 大阪
    • Year and Date
      2016-05-24
    • Related Report
      2016 Research-status Report
  • [Book] 流体計測法(改訂第2版)2019

    • Author(s)
      日本機械学会
    • Total Pages
      500
    • Publisher
      日本機械学会
    • Related Report
      2018 Annual Research Report
  • [Book] Reproductive and Developmental Strategies2018

    • Author(s)
      Matsuda M
    • Total Pages
      789
    • Publisher
      Springer
    • Related Report
      2018 Annual Research Report
  • [Book] PIVハンドブック(第2版)2018

    • Author(s)
      可視化情報学会
    • Total Pages
      480
    • Publisher
      森北出版
    • ISBN
      9784627671829
    • Related Report
      2018 Annual Research Report 2017 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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