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Plastic deforamation behaviours in dislocation free protein crystals

Research Project

Project/Area Number 16K06708
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Physical properties of metals/Metal-base materials
Research InstitutionYokohama Soei University

Principal Investigator

KOJIMA Kenichi  横浜創英大学, こども教育学部, 教授 (90046095)

Co-Investigator(Kenkyū-buntansha) 小泉 晴比古  名古屋大学, 未来材料・システム研究所, 特任講師 (10451626)
若生 啓  横浜創英大学, こども教育学部, 講師 (40515839)
橘 勝  横浜市立大学, 生命ナノシステム科学研究科(八景キャンパス), 教授 (80236546)
Project Period (FY) 2016-10-21 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords転位 / 結晶の力学的性質 / 結晶塑性 / 放射光X線トポグラフ / タンパク質結晶 / 微少硬さ / 物質の強度 / X線回折曲線 / 放射光X線トポグラフ / 結晶の強度 / 結晶の完全性 / 無転位結晶 / X線動力学効果 / ビッカース硬さ / 塑性 / 脆性 / X線回折 / 動力学的効果 / 格子欠陥 / 放射光トポグラフ / 力学的性質 / 結晶評価 / 新素材 / X線、粒子線 / 蛋白質 / 結晶成長
Outline of Final Research Achievements

In order to utilize protein crystals as engineering materials, the fundamental mechanical properties of protein crystals were studied. In general, the protein crystals are brittle. To investigate plastic deformation in protein crystals, the stress-induced dislocations in glucose isomerase (GI) crystals without dislocations are investigated by synchrotron X-ray topography. The stress is applied by simple indentation method. The clear dislocation images are observed after the indentation. Dislocations are nucleated from the indentation area and then they propagate to the inside. We confirmed that plastic deformation in protein crystals is controlled by dislocation motion and multiplication. Moreover, the observation of dynamical diffraction in GI crystals is carried out. The oscillation profiles of GI crystals are in agreement with those predicted by dynamical theory of diffraction.

Academic Significance and Societal Importance of the Research Achievements

学術的意義としては、タンパク質結晶の塑性変形が転位によって律速されていることを初めて明らかにしたことである。また、従来、タンパク質結晶は完全性が低く無転位結晶は得られないと考えられていたが、本研究により無転位かつ完全性の高いタンパク質結晶が得られ、転位の増殖の機構を解明するとともに、X線回折曲線を動力学回折理論によって説明できることを明らかにした。社会的意義としては、環境問題となっているプラスチックのような人工高分子の代替材料として、自然素材であるタンパク質結晶の工学的利用はこれからの社会にとって必要な事である。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (20 results)

All 2018 2017 2016

All Journal Article (9 results) (of which Peer Reviewed: 9 results,  Open Access: 4 results,  Acknowledgement Compliant: 4 results) Presentation (11 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Direct observation of stress-induced dislocations in protein crystals by synchrotron X-ray topography2018

    • Author(s)
      Suzuki Ryo、Tachibana Masaru、Koizumi Haruhiko、Kojima Kenichi
    • Journal Title

      Acta Materialia

      Volume: 156 Pages: 479-485

    • DOI

      10.1016/j.actamat.2018.06.018

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Importance of Hydration State around Proteins Required to Grow High-Quality Protein Crystals2018

    • Author(s)
      Haruhiko Koizumi, Satoshi Uda, Katsuo, Tsukamoto, Kenichi Kojima, Masaru Tachibana, Toru Ujihara
    • Journal Title

      Crystal Growth & Design

      Volume: 18 Issue: 8 Pages: 4749-4755

    • DOI

      10.1021/acs.cgd.8b00798

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of oscillatory rocking curve by dynamical diffraction in protein crystals2018

    • Author(s)
      Ryo Suzuki, Haruhiko Koizumi, Keiichi Hirano, Takashi Kumasaka, Kenichi Kojima and Masaru Tachibana
    • Journal Title

      Proceeding of National Academy of Sience

      Volume: 115 Issue: 14 Pages: 3634-3639

    • DOI

      10.1073/pnas.1720098115

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Microindentation Hardness of Protein Crystals under Controlled Relative Humidity2017

    • Author(s)
      Kishi Takeharu, Suzuki Ryo, Shigemoto Chika, Murata Hidenobu, Kojima Kenichi, Tachibana Masaru
    • Journal Title

      Crystals

      Volume: 7 Issue: 11 Pages: 339-339

    • DOI

      10.3390/cryst7110339

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Characterization of grown-in dislocations in high-quality glucose isomerase crystals by synchrotron monochromatic-beam X-ray topography2017

    • Author(s)
      Ryo Suzuki, Haruhiko Koizumi, Kenichi Kojima, Seijiro Fukuyama, Yasutomo Arai, Katsuo Tsukamoto, Yoshihisa Suzuki, Masaru Tachibana
    • Journal Title

      Journal of Crystal Growth

      Volume: in press Pages: 299-304

    • DOI

      10.1016/j.jcrysgro.2016.10.047

    • Related Report
      2017 Research-status Report 2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Crystallization Technique of High-Quality Protein Crystals Controlling Surface Free Energy2017

    • Author(s)
      H. Koizumi, S. Uda, K. Tsukamoto, M. Tachibana, K. Kojima, J. Okada and J. Nozawa
    • Journal Title

      Crystal Growth & Design

      Volume: 17 Issue: 12 Pages: 6712-6718

    • DOI

      10.1021/acs.cgd.7b01315

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Hardness and slip systems of orthorhombic hen egg-white lysozyme crystals2016

    • Author(s)
      Ryo Suzuki, Takeharu Kishi, Shiro Tsukashima, Masaru Tachibana, Kei Wako, Kenichi Kojima
    • Journal Title

      Philosophical Magazine

      Volume: 96 Issue: 28 Pages: 2930-2942

    • DOI

      10.1080/14786435.2016.1219074

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Importance of Determination of Crystal Quality in Protein Crystals when Performing High-Resolution Structural Analysis2016

    • Author(s)
      Koizumi, Haruhiko Suzuki, Ryo Tachibana, Masaru Tsukamoto, Katsuo Yoshizaki, Izumi Fukuyama, Seijiro Suzuki, Yoshihisa Uda, Satoshi Kojima, Kenichi
    • Journal Title

      Crystal Growth & Design

      Volume: 16 Issue: 9 Pages: 4905-4909

    • DOI

      10.1021/acs.cgd.6b00457

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] タンパク質結晶の放射光X線トポグラフィ2016

    • Author(s)
      橘勝,鈴木凌,小泉晴比古,若生啓,小島謙一
    • Journal Title

      日本結晶成長学会誌

      Volume: 43 Pages: 38-46

    • NAID

      130005997169

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] タンパク質結晶におけるX線の動力学的回折の初観測2018

    • Author(s)
      鈴木凌、小泉晴比古、平野馨一、熊坂崇、小島謙一、橘勝
    • Organizer
      第66回応用物理学会春季学術講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Micro-indentation hardness of Lysozyme crystals2018

    • Author(s)
      Ryo Suzuki, Chika Shigemoto, Marina Abe, Hidenobu Murata, Masaru Tachibana, Kenichi Kojima
    • Organizer
      6th International Indentation Workshop
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] X-ray topography of dislocations by indentation of protein crystals2018

    • Author(s)
      Ryo Suzuki, Chika Shigemoto, Marina Abe, Hidenobu Murata, Masaru Tachibana, Kenichi Kojima
    • Organizer
      6th International Indentation Workshop
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] タンパク質をモデルとした溶液成長における溶媒和構造と結晶性との相関2018

    • Author(s)
      小泉晴比古, 宇田聡, 塚本勝男, 橘勝, 小島謙一, 宇治原徹,
    • Organizer
      第47回結晶成長国内会議
    • Related Report
      2018 Annual Research Report
  • [Presentation] インデンテーションにより導入されたタンパク質結晶のプリズマティック転位の観察2018

    • Author(s)
      鈴木凌、村田秀信、小泉晴比古、小島謙一、橘勝
    • Organizer
      日本物理学会第73回年次大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 擬BCC構造を有するタンパク質結晶の転位の運動と塑性変形2017

    • Author(s)
      鈴木凌、村田秀信、小泉晴比古、塚本勝男、荒井康智、福山誠二郎、鈴木良尚、小島謙一、橘勝
    • Organizer
      日本物理学会 第72回年次大会
    • Place of Presentation
      大阪大学
    • Year and Date
      2017-03-17
    • Related Report
      2016 Research-status Report
  • [Presentation] ステップ自由エネルギー制御による高品質タンパク質結晶の育成2017

    • Author(s)
      小泉晴比古、宇田聡、塚本勝男、橘勝、小島謙一、岡田純平、野澤純
    • Organizer
      第46回結晶成長国内会議(JCCG-46)(静岡県浜松市, ホテルコンコルド浜松)
    • Related Report
      2017 Research-status Report
  • [Presentation] ロッキングカーブ測定によるタンパク質結晶の動力学的回折の観測2017

    • Author(s)
      鈴木凌、村田秀信、小泉晴比古、平野馨一、杉山弘、熊坂崇、小島謙一、橘勝
    • Organizer
      日本物理学会2017年秋季大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 三斜晶リゾチーム結晶の微小硬さと塑性変形2017

    • Author(s)
      重本知華、鈴木凌、村田秀信、小島謙一、橘勝
    • Organizer
      日本物理学会2017年秋季大会
    • Related Report
      2017 Research-status Report
  • [Presentation] タンパク質結晶におけるX線ロッキングカーブの振動強度の観測2017

    • Author(s)
      鈴木凌、小泉晴比古、平野馨一、熊坂崇、小島謙一、橘勝
    • Organizer
      平成29年度日本結晶学会年会
    • Related Report
      2017 Research-status Report
  • [Presentation] グルコースイソメラーゼ結晶の動力学的回折のロッキングカーブ測定2017

    • Author(s)
      鈴木凌、小泉晴比古、平野馨一、熊坂崇、小島謙一、橘勝
    • Organizer
      第46回結晶成長国内会議(JCCG-46)
    • Related Report
      2017 Research-status Report

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Published: 2016-10-24   Modified: 2020-03-30  

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