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Development of surface potential-controlled Ti with high osteoblast activity and hydrophilicity

Research Project

Project/Area Number 16K06786
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Structural/Functional materials
Research InstitutionJapan Fine Ceramics Center

Principal Investigator

Hashimoto Masami  一般財団法人ファインセラミックスセンター, その他部局等, 上級研究員 (20450851)

Co-Investigator(Kenkyū-buntansha) 金高 弘恭  東北大学, 歯学研究科, 准教授 (50292222)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords表面電位 / 酸化チタン / 窒素含有欠陥 / 骨芽細胞 / 水酸化アパタイト / アパタイト / 表面電荷 / タンパク質 / 分化 / 親水性 / チタン / 生体材料
Outline of Final Research Achievements

MC3T3-E1 cell differentiation and related surface potentials of rutile-type TiO2 scales formed on Ti are controlled by varying the Ti heat treatment conditions in a N2 atmosphere containing a trace amount of O2. The zeta potentials of the samples heated at 873 and 973 K for 1 h show large negative and positive values, respectively, while cell differentiation on the surface is enhanced in both cases (14 days incubation). In the case of untreated Ti, the cell differentiation diminishes and the zeta potential becomes more neutral. Protein detection and Ca and P detection, rather than an adhesive protein such as fibronectin, predominantly adsorbed on the scales formed in 1 h at 873 and 973 K, respectively. In the case of untreated Ti, fibronectin , but no Ca and P were detected. The present findings illuminate the relationship between charged surfaces and MC3T3-E1 cellular response.

Academic Significance and Societal Importance of the Research Achievements

純チタンの生体活性に関しては、酸素分圧を制御した窒素雰囲気中での熱処理によって酸化チタン中の窒素含有欠陥を制御した報告例は無く、窒素含有欠陥の有効電荷を変化させる取り組みも我々のグループ以外には無い独創的なものである。さらに純チタンに吸着するタンパク質の形態も表面電位の違いによって異なると考えられ、純チタンの窒素含有欠陥制御→表面電位制御→タンパク質吸着形態解明といった一連の生体分子の吸着課程の経時変化が明確となり、生体活性能の本質を理解した材料設計を可能にした。
本技術は、健康寿命を延伸し、生活の質を生涯にわたり保つことが可能になるばかりでなく、医療費、介護サービス費の削減が期待できる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (18 results)

All 2019 2018 2017 2016

All Journal Article (8 results) (of which Peer Reviewed: 7 results,  Open Access: 1 results) Presentation (10 results) (of which Int'l Joint Research: 6 results,  Invited: 1 results)

  • [Journal Article] MC3T3-E1 cellular response and protein detection on surface potential-controlled TiO2 scale in serum-containing medium2018

    • Author(s)
      M. Hashimoto, T. Ogawa, S. Kitaoka, S. Muto, M. Furuya, H Kanetaka, M. Abe and H. Yamashita
    • Journal Title

      Key Eng. Mat

      Volume: 782 Pages: 218-223

    • DOI

      10.4028/www.scientific.net/kem.782.218

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Control of surface potential and hydroxyapatite formation on TiO 2 scales containing nitrogen-related defects2018

    • Author(s)
      Hashimoto Masami、Ogawa Takafumi、Kitaoka Satoshi、Muto Shunsuke、Furuya Maiko、Kanetaka Hiroyasu、Abe Masayuki、Yamashita Hayato
    • Journal Title

      Acta Materialia

      Volume: 155 Pages: 379-385

    • DOI

      10.1016/j.actamat.2018.05.072

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hydrated Phospholipid Polymer Gel-Like Layer for Increased Durability of Orthopedic Bearing Surfaces2018

    • Author(s)
      Kyomoto Masayuki、Moro Toru、Yamane Shihori、Watanabe Kenichi、Hashimoto Masami、Tanaka Sakae、Ishihara Kazuhiko
    • Journal Title

      Langmuir

      Volume: 35 Issue: 5 Pages: 1954-1963

    • DOI

      10.1021/acs.langmuir.8b01494

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A phospholipid polymer graft layer affords high resistance for wear and oxidation under load bearing conditions2018

    • Author(s)
      Kyomoto Masayuki、Moro Toru、Yamane Shihori、Watanabe Kenichi、Hashimoto Masami、Tanaka Sakae、Ishihara Kazuhiko
    • Journal Title

      Journal of the Mechanical Behavior of Biomedical Materials

      Volume: 79 Pages: 203-212

    • DOI

      10.1016/j.jmbbm.2017.12.036

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Control of HAp formation and osteoconductivity on nitrogen-doped TiO2 scale formed by oxynitridation of Ti2017

    • Author(s)
      3.Masami Hashimoto, Satoshi Kitaoka, Yoshihiro Obata, Shunsuke Muto, Takafumi Ogawa, Maiko Furuya, Hiroyasu Kanetaka
    • Journal Title

      Key Engineering Materials

      Volume: 758 Pages: 86-89

    • DOI

      10.4028/www.scientific.net/kem.758.86

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] PMMA骨セメント用高生体活性酸化チタン粒子の開発2017

    • Author(s)
      橋本雅美, 金高弘恭
    • Journal Title

      FCレポート

      Volume: 35 Pages: 78-81

    • NAID

      40021193468

    • Related Report
      2017 Research-status Report
  • [Journal Article] The microstructure of scale formed by oxynitriding of Ti and exhibiting significant apatite-forming ability2016

    • Author(s)
      Masami Hashimoto, Satoshi Kitaoka, Shunsuke Muto, Kazuyoshi Tatsumi, Yoshihiro Obata
    • Journal Title

      Journal of Materials Research

      Volume: 31 Issue: 8 Pages: 1004-1011

    • DOI

      10.1557/jmr.2016.79

    • NAID

      120005844138

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effect of surface charge of TiO2 particles on hydroxyapatite formation in simulated body fluid2016

    • Author(s)
      Masami Hashimoto, Satoshi Kitaoka and Hiroyasu Kanetaka
    • Journal Title

      Advanced Powder Technology

      Volume: 27 Issue: 6 Pages: 2409-2415

    • DOI

      10.1016/j.apt.2016.08.019

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] Differentiation behavior of osteogenic cell on surface potential-controlled TiO2 scale formed by oxynitridation of Ti2019

    • Author(s)
      M. Hashimoto, T. Ogawa, S. Kitaoka, *M. Furuya, *H. Kanetaka, *S. Muto, *M. Abe, *H. Yamashita
    • Organizer
      43rd international conference and exposition on advanced ceramics and composites,
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] MC3T3-E1 cellular response and protein detection on surface potential-controlled TiO2 scale in serum-containing medium2018

    • Author(s)
      M. Hashimoto, T. Ogawa, S. Kitaoka, S. Muto, M. Furuya, H. Kanetaka, M. Abe and H. Yamashita
    • Organizer
      Bioceramics30
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 血清含有培養液中における表面電位制御チタン上でのタンパク質の吸着2018

    • Author(s)
      橋本雅美,小川貴史,北岡諭,古谷真衣子,金高弘恭,武藤俊介,阿部真之,山下隼人
    • Organizer
      日本セラミックス協会第31回秋季シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] PMMA骨セメント用の高生体活性酸化チタン粒子の開発2018

    • Author(s)
      橋本雅美, 金高弘恭
    • Organizer
      JFCAテクノフェスタ
    • Related Report
      2018 Annual Research Report
  • [Presentation] 酸窒化処理による骨芽細胞活性の高い表面電荷制御チタンの開発2017

    • Author(s)
      橋本雅美, 北岡諭, 小幡佳弘, 武藤俊介, 古谷真衣子, 金高弘恭
    • Organizer
      日本材料学会第66期学術講演会
    • Related Report
      2017 Research-status Report
  • [Presentation] HAp formation and osteoconductivity of nitrogen-doped TiO2 scale formed by oxynitridation of Ti exhibiting different surface charge2017

    • Author(s)
      M. Hashimoto, S. Kitaoka, Y. Obata, S. Muto, M. Furuya, H. Kanetaka
    • Organizer
      IUMRS
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 窒素含有欠陥を含む酸化チタンスケールを介した金属チタンの生体活性制御2017

    • Author(s)
      橋本雅美, 小川貴史, 北岡諭, 武藤俊介, 古谷真衣子, 金高弘恭, 阿部真之, 山下隼人
    • Organizer
      日本セラミックス協会第30回秋季シンポジウム
    • Related Report
      2017 Research-status Report
  • [Presentation] Control of HAp formation and osteoconductivity on nitrogen-doped TiO2 scale formed by oxynitridation of Ti2017

    • Author(s)
      Masami Hashimoto, Satoshi Kitaoka, Yoshihiro Obata, Shunsuke Muto, Takafumi Ogawa, Maiko Furuya, Hiroyasu Kanetaka
    • Organizer
      Bioceramics29
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Control of Bioactivity of Ti Metal by intervening TiO2 Scale Containing Nitrogen-related Defects2017

    • Author(s)
      Masami Hashimoto, Satoshi Kitaoka, Takafumi Ogawa, Shunsuke Muto, Maiko Furuya, Hiroyasu Kanetaka, Masayuki Abe, Hayato Yamashita
    • Organizer
      International Symposium on Biomedical and Environmental Materials
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Comparison of apatite forming ability appeared by heat treatment TiO2 and Ti under PO2-controlled nitrogen atmosphere2016

    • Author(s)
      Masami Hashimoto, Satoshi Kitaoka, Shunsuke Muto,
    • Organizer
      AMTC5
    • Place of Presentation
      ウィンクあいち(愛知県名古屋市)
    • Year and Date
      2016-05-11
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research

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Published: 2016-04-21   Modified: 2020-03-30  

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