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Measurements of shear rate distribution and surface charge of bubbles in airlift bubble columns on the basis of structural change in lipid membranes

Research Project

Project/Area Number 16K06827
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Properties in chemical engineering process/Transfer operation/Unit operation
Research InstitutionYamaguchi University

Principal Investigator

Yoshimoto Makoto  山口大学, 大学院創成科学研究科, 准教授 (80322246)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsエアリフト型気泡塔 / せん断速度 / 気泡 / リポソーム / せん断ストレス / 脂質膜 / 表面電荷 / 脂質二分子膜 / 化学工学 / 生物・生体工学 / ナノバイオ
Outline of Final Research Achievements

Bubble column bioreactors are operated by dispersing bubbles in liquid phase. Quantitative evaluation of shear rate and electrostatic property of bubbles is of importance to rationally design various separation and reaction processes using bubble columns. In this work, the shear rate generated in various types of external loop airlift bubble column was estimated on the basis of the release kinetics of the hydrophilic dye molecules from liposomes. Furthermore, the electrostatic property of bubbles was also evaluated on the basis of membrane permeability of various charged liposomes suspended in the bubble columns.

Academic Significance and Societal Importance of the Research Achievements

液体せん断ストレスは,生細胞の活性や分子集合体から形成される人工材料の構造と機能に影響を及ぼす。本研究ではこの現象に着目して,構造的に柔軟な脂質二分子膜が気液二相流から受ける影響を,脂質膜透過係数の変化に基づいて定量化して,せん断速度の推定に利用している。この知見を応用すると,せん断ストレスや気泡を利用してリポソーム内と外部の物質移動を制御できるため,リポソーム内に閉じ込めた酵素を反応器の流動特性に基づいて活性化する新しい化学反応プロセスの構築等への展開が期待できる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (13 results)

All 2019 2018 2017 2016

All Journal Article (4 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (9 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] High permeability of polyunsaturated lipid bilayers as applied to attoliter enzyme reactors2019

    • Author(s)
      Naoyuki Nagatomo and Makoto Yoshimoto
    • Journal Title

      ACS Applied Bio Materials

      Volume: - Issue: 6 Pages: 2453-2463

    • DOI

      10.1021/acsabm.9b00165

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of Hydrodynamic Properties of Bubble Columns Based on Membrane Permeability of Liposomes2018

    • Author(s)
      中野 佑典, 吉本 誠
    • Journal Title

      BUNSEKI KAGAKU

      Volume: 67 Issue: 12 Pages: 711-717

    • DOI

      10.2116/bunsekikagaku.67.711

    • NAID

      130007554770

    • ISSN
      0525-1931
    • Year and Date
      2018-12-05
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Reactive bienzyme systems fabricated through immobilization of biotinylated glucose oxidase and peroxidase molecules onto neutralized avidin-conjugated liposomes2017

    • Author(s)
      Takeshi Ohtsu, Saki Shigenari, Makoto Yoshimoto and Hiroshi Umakoshi
    • Journal Title

      Biochemical Engineering Journal

      Volume: 125 Pages: 81-87

    • DOI

      10.1016/j.bej.2017.05.023

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Characteristics of bilayer membranes of vesicles composed of highly unsaturated fatty acids and phospholipids2017

    • Author(s)
      Naoyuki Nagatomo, Mizuki Yamaguchi and Makoto Yoshimoto
    • Journal Title

      Proceedings of 3rd International Symposium on Multiscale Multiphase Process Engineering

      Volume: 1 Pages: 327-330

    • Related Report
      2017 Research-status Report
  • [Presentation] 多価不飽和リン脂質ベシクルの特性を利用した酵素反応の制御2019

    • Author(s)
      吉本誠,長友尚之
    • Organizer
      化学工学会第84年会(東京)
    • Related Report
      2018 Annual Research Report
  • [Presentation] 疎水鎖長が異なる多価不飽和リン脂質から形成されるリポソームの膜透過性2018

    • Author(s)
      長友尚之,吉本誠
    • Organizer
      化学工学会第50回秋季大会(鹿児島)
    • Related Report
      2018 Annual Research Report
  • [Presentation] 気泡を利用したリン脂質ベシクルからの親水性物質の放出促進2018

    • Author(s)
      吉本誠,藤江徹弥,中野佑典
    • Organizer
      化学工学会第50回秋季大会(鹿児島)
    • Related Report
      2018 Annual Research Report
  • [Presentation] リポソームを利用した外部循環式エアリフト型気泡塔のせん断速度と気泡表面電荷の推定2017

    • Author(s)
      中野佑典,吉本誠
    • Organizer
      化学工学会中国四国支部大会山口大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 気泡群共存下におけるリポソームの安定性に及ぼすPEG化脂質の効果2017

    • Author(s)
      藤江徹弥,吉本誠
    • Organizer
      化学工学会中国四国支部大会山口大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Shear sensitivity of liposomes mimicking biological membranes2016

    • Author(s)
      Makoto Yoshimoto and Tetsuya Fujie
    • Organizer
      The 22nd Symposium of Young Asian Biological Engineers' Community
    • Place of Presentation
      Phoenix Seagaia Resort (Miyazaki, Japan)
    • Year and Date
      2016-10-27
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] リポソームを用いた装置特性に依存するエアリフト型気泡塔内せん断速度の推定2016

    • Author(s)
      中野佑典,吉本誠
    • Organizer
      化学工学会第10回中四国若手CE合宿
    • Place of Presentation
      ホテル常盤(山口県山口市)
    • Year and Date
      2016-09-28
    • Related Report
      2016 Research-status Report
  • [Presentation] 気泡群共存下における生体膜組成をもつリポソームの安定性2016

    • Author(s)
      藤江徹弥,吉本誠
    • Organizer
      化学工学会第10回中四国若手CE合宿
    • Place of Presentation
      ホテル常盤(山口県山口市)
    • Year and Date
      2016-09-28
    • Related Report
      2016 Research-status Report
  • [Presentation] リン脂質ベシクルの構造柔軟性を利用したエアリフト気泡塔内流動特性の推定2016

    • Author(s)
      吉本誠,中野佑典,夏目友誉
    • Organizer
      混相流シンポジウム2016
    • Place of Presentation
      同志社大学(京都府京都市)
    • Year and Date
      2016-08-08
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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