| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
The purpose of this study was to synthesize a large amount of a novel carotenoid with high antioxidant activity by extremely halophilic archaeon Haloarcula japonica.
In order to synthesize carotenoids with more antioxidant activity, we aimed to synthesize tetradehydrolycopene with 15 conjugated double bonds. For its synthesis, carotenoid desaturase (CrtD) was mutated by the directed evolution. Analysis of the mutated CrtD gene library revealed amino acid residues important for lycopene synthesis.
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