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Differentiation switch phenomena of iPS cells depending on culture oxygen environments

Research Project

Project/Area Number 16K06875
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionThe University of Kitakyushu

Principal Investigator

Nakazawa Kohji  北九州市立大学, 国際環境工学部, 教授 (00304733)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
KeywordsiPS細胞 / 胚様体 / マイクロウェルチップ / 酸素 / 細胞分化 / 細胞増殖 / PMMA / PDMS / マウスiPS細胞 / ヒトiPS細胞 / 低酸素環境 / 分化 / 低酸素 / Wntシグナル / ハンギングドロップ / 酸素環境 / 増殖
Outline of Final Research Achievements

In this study, we investigated that the effects of culture oxygen environments on embryoid body (EB) differentiation of iPS cells. As a culture platform, we designed PMMA microwell chip (low oxygen permeability) and PDMS microwell chip (high oxygen permeability), and then mouse iPS cells cultured in these chips. The cell proliferation and hypoxia condition of the EBs in PDMS chip were improved than those in PMMA chip. Furthermore, the vascular and hepatic differentiations of EBs were promoted in the PDMS chip and PMMA chip cultures, respectively. These results indicate that the differentiation fates of stem cells are varied by the difference of oxygen environment surrounding EBs.

Academic Significance and Societal Importance of the Research Achievements

再生医療や細胞アッセイなどの技術開発では、細胞特性を培養下でいかに制御するかが重要である。ここで、培養細胞の特性は栄養素、酸素、サプリメント、細胞代謝物などの様々な環境因子の影響を受ける。本研究では細胞を取り巻く「酸素環境」に着目し、酸素供給能が異なる独自の培養プラットフォームを作製してiPS細胞の特性を評価した。その結果、酸素環境の違いによって細胞の増殖性や分化状態が大きく変動することを明らかにした。これらの成果は、細胞特性を制御するための培養環境設計に重要な指針を与えるものである。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (25 results)

All 2019 2018 2017 2016 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (15 results) (of which Int'l Joint Research: 7 results) Book (5 results) Remarks (2 results)

  • [Journal Article] Changes in HepG2 spheroid behavior induced by differences in the gap distance between spheroids in a micropatterned culture system2018

    • Author(s)
      Miyamoto Daisuke、Hara Takuya、Hyakutake Ai、Nakazawa Kohji
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 印刷中 Issue: 6 Pages: 729-735

    • DOI

      10.1016/j.jbiosc.2017.12.013

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Spheroid culture enhances osteogenic potential of periodontal ligament mesenchymal stem cells2018

    • Author(s)
      Moritani Y.、Usui M.、Sano K.、Nakazawa K.、Hanatani T.、Nakatomi M.、Iwata T.、Sato T.、Ariyoshi W.、Nishihara T.、Nakashima K.
    • Journal Title

      Journal of Periodontal Research

      Volume: 53 Issue: 5 Pages: 870-882

    • DOI

      10.1111/jre.12577

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differentiation of mouse iPS cells is dependent on embryoid body size in microwell chip culture2016

    • Author(s)
      D. Miyamoto, K. Nakazawa
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 122 Issue: 4 Pages: 507-512

    • DOI

      10.1016/j.jbiosc.2016.03.018

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] 2D/3D培養における間葉系幹細胞の特性2018

    • Author(s)
      徳永優斗, 宮本大輔, 中澤浩二
    • Organizer
      第55回化学関連支部合同九州大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 酸素透過性マイクロウェルチップを用いたスフェロイド培養2018

    • Author(s)
      宮本大輔, 中澤浩二
    • Organizer
      化学工学会 第50回秋季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 間葉系幹細胞の2D/3D 培養2018

    • Author(s)
      徳永優斗, 宮本大輔, 中澤浩二
    • Organizer
      化学工学会 第50回秋季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] PDMS 製マイクロウェルチップを用いたiPS 胚様体培養2018

    • Author(s)
      宮本大輔, 中澤浩二
    • Organizer
      第40回日本バイオマテリアル学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 単層/スフェロイド培養における間葉系幹細胞の特性比較2018

    • Author(s)
      徳永優斗, 宮本大輔, 中澤浩二
    • Organizer
      第40回日本バイオマテリアル学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Morphological and functional studies of co-cultured hepatocyte spheroids with fibroblasts in a microwell chip culture2018

    • Author(s)
      K. Nakazawa, D. Miyamoto, O. Kitano
    • Organizer
      2018 ASCB/EMBO Meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Embryoid body culture of mouse iPS cells using microwell chips with different oxygen permeability2018

    • Author(s)
      D. Miyamoto, K. Nakazawa
    • Organizer
      2018 ASCB/EMBO Meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Neuronal differentiation of NT2 cells in two-dimensional monolayer and three-dimensional spheroid cultures2018

    • Author(s)
      O. Kitano, D. Miyamoto, K. Nakazawa
    • Organizer
      2018 ASCB/EMBO Meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 酸素透過性マイクロウェルチップを用いたマウスiPS胚様体培養2017

    • Author(s)
      宮本大輔, 山口健太, 中澤浩二
    • Organizer
      化学工学会 第49回秋季大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Embryoid body culture of mouse iPS cells using an oxygen-permeable microwell chip2017

    • Author(s)
      D. Miyamoto, K. Nakazawa
    • Organizer
      2017 TERMIS-Americas
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Embryoid body culture of mouse iPS cells using PDMS microwell chip2016

    • Author(s)
      K. Yamaguchi, D. Miyamoto, K. Nakazawa
    • Organizer
      The 11th SPSJ International Polymer Conference(IPC2016)
    • Place of Presentation
      福岡国際会議場(福岡県・福岡市)
    • Year and Date
      2016-12-13
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Characteristics of mouse iPS embryoid bodies cultured on a microwell chip2016

    • Author(s)
      D. Miyamoto, K. Nakazawa
    • Organizer
      2016 TERMIS-AM
    • Place of Presentation
      Manchester Grand Hyatt San Diego,サンディエゴ(米国)
    • Year and Date
      2016-12-11
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] iPS胚様体を取り巻く酸素環境と幹細胞分化特性の関係2016

    • Author(s)
      山口健太, 宮本大輔, 中澤浩二
    • Organizer
      日本バイオマテリアル学会シンポジウム2016
    • Place of Presentation
      福岡国際会議場(福岡県・福岡市)
    • Year and Date
      2016-11-21
    • Related Report
      2016 Research-status Report
  • [Presentation] 培養酸素環境がiPS胚様体の分化特性に与える効果2016

    • Author(s)
      宮本大輔, 中澤浩二
    • Organizer
      日本動物実験代替法学会第29回大会
    • Place of Presentation
      九州大学百年講堂(福岡県・福岡市)
    • Year and Date
      2016-11-16
    • Related Report
      2016 Research-status Report
  • [Presentation] Effect of oxygen environment on embryoid body culture of mouse iPS cells2016

    • Author(s)
      K. Yamaguchi, D. Miyamoto, K. Nakazawa
    • Organizer
      2016 TERMIS-AP
    • Place of Presentation
      Fullon Hotel Tamsui,台北市(台湾)
    • Year and Date
      2016-09-03
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Book] 三次元培養における培養手法と周辺技術動向2019

    • Author(s)
      井嶋博之、水本、博、中澤浩二、他27名
    • Total Pages
      197
    • Publisher
      情報機構
    • ISBN
      9784865021684
    • Related Report
      2018 Annual Research Report
  • [Book] 臓器チップの技術と開発動向2018

    • Author(s)
      酒井康行、金森敏幸、中澤浩二、他46名
    • Total Pages
      293
    • Publisher
      シーエムシー出版
    • ISBN
      9784781313252
    • Related Report
      2018 Annual Research Report
  • [Book] 臓器チップの技術と開発動向2018

    • Author(s)
      酒井康行、金森敏幸、小島肇夫、中澤浩二、他
    • Total Pages
      293
    • Publisher
      シーエムシー出版
    • Related Report
      2017 Research-status Report
  • [Book] 細胞培養の基礎知識と細胞培養基材の利用・開発の留意点2016

    • Author(s)
      田畑泰彦、小原有弘、福田隆之、古江-楠田美保、林洋平、清水則夫、西條薫、藤岡剛、三浦巧、佐藤陽治、山本雅哉、田中賢、中澤浩二、土屋勝則、藤本興治、掛川貴弘、木戸秋悟、篠原満利恵、木村圭一、他
    • Total Pages
      200
    • Publisher
      情報機構
    • Related Report
      2016 Research-status Report
  • [Book] Advances in Microfluidics - New Applications in Biology, Energy, and Materials Sciences2016

    • Author(s)
      V. Houbart, M. Fillet, E. Roy, A. Pallandre, B. Zribi, M.C. Horny, F.D. Delapierre, A. Cattoni, J. Gamby, A.M. Haghiri-Gosnet, S. Sugiura, K. Nakazawa, T. Kanamori, K. Ohnuma, 他
    • Total Pages
      420
    • Publisher
      INTECH
    • Related Report
      2016 Research-status Report
  • [Remarks] 北九州市立大学 中澤研究室

    • URL

      http://chempro.env.kitakyu-u.ac.jp/~knakazawa/

    • Related Report
      2018 Annual Research Report
  • [Remarks] 北九州市立大学 中澤研究室

    • URL

      http://chempro.env.kitakyu-u.ac.jp/~knakazawa/index.html

    • Related Report
      2017 Research-status Report 2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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