• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Investigation of biovolatilization mechanism in rare metal metabolizing bacterium and development of biovolatile compounds recovery

Research Project

Project/Area Number 16K06876
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionShibaura Institute of Technology

Principal Investigator

Yamashita Mitsuo  芝浦工業大学, 工学部, 教授 (40220347)

Research Collaborator Horiike Takumi  
Otsuka Osamu  
Fukazawa Fumito  
Kotake Fumiya  
Udagawa Kento  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsレアメタル / セレン / 生物気化 / メカニズム / 回収
Outline of Final Research Achievements

Selenate reducing bacterium, Pseudomonas stutzeri strain NT-I reduce selenate, selenite, and biogenic elemental selenium into volatile selenium (Se), dimethyldiselenide (DMDSe). DMDSe was recovered at about 39% in nitric acid from waste water containing Se using strain NT-I. The biogenic elemental selenium was recycled at 99.7% purity from Se compounds in nitric acid by a sequence of reaction of neutralization, oxidation and reduction. The recombinant E. coli DH5a (lac-ME-His) was constructed harbouring DMDSe synthesis gene under lactose promoter. The recombinant E. coli DH5a (lac-ME-His) reduced biogenic elemental selenium and synthesized volatile DMDSe as same level as strain NT-I. Taken together, we succeeded in development of selenium recovery technology including recombinant DMDSe synthesis gene from waste water.

Academic Significance and Societal Importance of the Research Achievements

太陽電池に使用されているレアメタルの1種類であるセレンに注目し、セレン還元細菌Pseudomonas stutzeri strain NT-Iの気化能(セレンバイオボラタリゼーション)を活用してセレンを含む廃水から高純度の元素態セレンを精錬し、浄化回収資源化技術開発に成功した。生物由来の特異なバイオボラタリゼーションを利用したレアメタル浄化回収技術は廃水や廃棄物から有価資源を回収するという特色のある手法であり、「循環型社会」形成の一助となると期待できる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (17 results)

All 2018 2017 2016 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (13 results) (of which Int'l Joint Research: 2 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Fundamental Studies on Oxidizing Roasting of the “Bioselenium”2016

    • Author(s)
      Osamu Otsuka, Yutaka Yanaba, Takeshi Yoshikawa, and Mitsuo Yamashita
    • Journal Title

      MATERIALS TRANSACTIONS

      Volume: 57 Issue: 7 Pages: 1183-1191

    • DOI

      10.2320/matertrans.M2016060

    • NAID

      130005158409

    • ISSN
      1345-9678, 1347-5320
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Bioremediation of selenate-contaminated soils using Pseudomonas stutzeri NT-I2018

    • Author(s)
      Osamu Otsuka, Ryo Nishizato, Minoru Okuno, Hiroyuki Mutou, Naoto Watanabe, Tsutomu Matsuo, Fumiya Kotake, Mitsuo Yamashita
    • Organizer
      The 7th International Selenium Conference (Se2018) Selenium in Biology, Chemistry and Medicine
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] セレン酸還元細菌内包ポリマーカプセルによる新たなセレン廃水の浄化と資源化2018

    • Author(s)
      平谷朋之、小竹史哉、永直文、大塚治、山下光雄
    • Organizer
      日本生物工学会2018年度大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] バイオレメディエーションによるセレン汚染土壌浄化2018

    • Author(s)
      山下光雄、小竹史哉、大塚治、西里亮、奥野稔、武藤宏行
    • Organizer
      日本生物工学会2018年度大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 好気性セレン酸還元細菌Pseudomonas stutzeri NT-I株のDimethyl Diselenide合成特徴(その2)2018

    • Author(s)
      山下光雄、大塚 治、黒田真史、池道彦
    • Organizer
      第 4 回日本セレン研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] セレン酸還元細菌Pseudomonas stutzeri NT-I株を利用したセレン汚染土壌の不溶化処理(その3)2018

    • Author(s)
      小竹史哉、大塚治、山下光雄、西里亮、奥野稔、武藤宏行、佐々木雄一
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Research-status Report
  • [Presentation] セレン酸還元細菌Pseudomonas stutzeri NT-I株を利用したセレン汚染土壌の不溶化処理(その1)2017

    • Author(s)
      福澤文人、大塚治、山下光雄、西里亮、奥野稔、武藤宏行
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2017-03-18
    • Related Report
      2016 Research-status Report
  • [Presentation] セレン酸還元細菌Pseudomonas stutzeri NT-I株を利用したセレン汚染土壌の不溶化処理(その2)2017

    • Author(s)
      大塚治、福澤文人、山下光雄、西里亮、奥野稔、武藤宏行
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2017-03-18
    • Related Report
      2016 Research-status Report
  • [Presentation] Pseudomonas stutzeri NT-Iのセレン酸還元関連遺伝子群の同定と解析2017

    • Author(s)
      黒田真史、櫻井柴乃、池道彦、山下光雄
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2017-03-18
    • Related Report
      2016 Research-status Report
  • [Presentation] セレン酸還元細菌Pseudomonas stutzeri NT-I株によるセレン酸汚染土壌のバイオレメディエーション2017

    • Author(s)
      大塚治、福澤文人、堀池巧、山下光雄、西里亮、奥野稔、武藤宏行
    • Organizer
      土木学会平成29年度全国大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Pseudomonas stutzeri NT-Iのセレン酸還元関連遺伝子群の同定と解析2017

    • Author(s)
      黒田真史、櫻井柴乃、山下光雄、池道彦
    • Organizer
      第69回日本生物工学会大会(2017)
    • Related Report
      2017 Research-status Report
  • [Presentation] 好気性セレン酸還元細菌Pseudomonas stutzeri NT-I株のDimethyl Diselenide合成特徴2017

    • Author(s)
      大塚 治、黒田雅史、池道彦、山下光雄
    • Organizer
      第 3 回日本セレン研究会
    • Related Report
      2017 Research-status Report
  • [Presentation] 海洋性微生物のセレン・テルル還元能の特徴解析2017

    • Author(s)
      堀池巧、寺原猛、今田千秋、山下光雄
    • Organizer
      第 3 回日本セレン研究会
    • Related Report
      2017 Research-status Report
  • [Presentation] Optimization of Dimethyl diselenide synthesis by Pseudomonas stutzeri NT-I2016

    • Author(s)
      Osamu Otsuka, Masashi Kuroda, Michihiko Ike, and Mitsuo Yamashita
    • Organizer
      13th International Conference on the Chemistry of Seleniumu and Tellurium
    • Place of Presentation
      Nagaragawa Convention Center at Gifu, Japan
    • Year and Date
      2016-05-17
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Book] Microbial Removal and Recovery of Metals from Wastewater2018

    • Author(s)
      Michihiko Ike, Mitsuo Yamashita, Masashi Kuroda
    • Total Pages
      595
    • Publisher
      Adavanced Biotechnology Vol. 5. Wiley Biotechnology Series
    • ISBN
      9783527340750
    • Related Report
      2018 Annual Research Report
  • [Book] Removal and Recovery of Metals from Wastewater. entitled "Metal resources recovery from wastes". Applied Bioengineering (Adavanced Biotechnology Vol. 5)2017

    • Author(s)
      Michihiko Ike, Mitsuo Yamashita, Masashi Kuroda
    • Total Pages
      623
    • Publisher
      WILEY-VCH
    • Related Report
      2016 Research-status Report
  • [Remarks] レアメタルバイオリサーチセンター

    • URL

      http://www.sic.shibaura-it.ac.jp/^yamashi/

    • Related Report
      2017 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi