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Noval regulatory mechanisms of axonal action potentials

Research Project

Project/Area Number 16K07006
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Neurophysiology / General neuroscience
Research InstitutionDoshisha University

Principal Investigator

Misono Hiroaki  同志社大学, 脳科学研究科, 教授 (40609509)

Research Collaborator Hirono Moritoshi  同志社大学, 研究開発推進機構, 特定任用研究員 (30318836)
Watanabe Shoji  同志社大学, 研究開発推進機構, 特定任用研究員 (80462745)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsイオンチャネル / 軸索 / 活動電位 / 小脳 / 興奮 / 神経興奮 / 運動学酒 / 神経軸索
Outline of Final Research Achievements

This research project aimed to elucidate the novel regulatory mechanisms of axonal action potentials by calcium signaling and calcium-dependent ion channels. Particularly, we aimed to develop new methods to identify and quantify axonal ion channels using various microscopy techniques including electron microscopy and super-resolution microscopy, to study how ion channels localize to the axon, and to develop reliable markers of the axons. We have obtained results indicating molecular mechanisms of ion channel localization and new properties of an axonal marker, tau protein, which is a causative factor of Alzheimer's disease.

Academic Significance and Societal Importance of the Research Achievements

本研究は、新しい神経メカニズム「軸索可塑性」の基盤になると考えられる軸索Ca2+シグナリングとその生理学的役割を明らかにすることを目的として行われた。軸索Ca2+シグナリングの役割が明らかになれば、神経細胞の計算能力やネットワークの特性をより深く理解することができる。また、本研究で開発した、電子顕微鏡及び超高解像顕微鏡を用いた軸索イオンチャネル検出法は、これまで困難であった軸索研究に道を開くものである。さらに、軸索タウタンパク質は、アルツハイマー病の原因因子であり、正常神経細胞での動態が明らかになったことにより、正常から病態へのタウの変容を、これまでより詳細に検討することができるようになる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (10 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (3 results) Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 1 results) Presentation (3 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Int'l Joint Research] IST Austria(オーストリア)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] IST Austria(Austria)

    • Related Report
      2017 Research-status Report
  • [Int'l Joint Research] IST Austria(Austria)

    • Related Report
      2016 Research-status Report
  • [Journal Article] Distribution of endogenous normal tau in the mouse brain.2019

    • Author(s)
      Kubo A, Misonou H, Matsuyama M, Nomori A, Wada-Kakuda S, Takashima A, Kawata M, Murayama S, Ihara Y, Miyasaka T.
    • Journal Title

      Journal of Comparative Neurology

      Volume: 527 Pages: 985-998

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Perineuronal Nets in the Deep Cerebellar Nuclei Regulate GABAergic Transmission and Delay Eyeblink Conditioning.2018

    • Author(s)
      Hirono M, Watanabe S, Karube F, Fujiyama F, Kawahara S, Nagao S, Yanagawa Y, Misonou H.
    • Journal Title

      J Neurosci.

      Volume: 38 Issue: 27 Pages: 6130-6144

    • DOI

      10.1523/jneurosci.3238-17.2018

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Precise localizations of voltage-gated sodium and potassium channels in neurons.2018

    • Author(s)
      Misonou H.
    • Journal Title

      Developmental Neuroscience

      Volume: 78 Issue: 3 Pages: 271-282

    • DOI

      10.1002/dneu.22565

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Trafficking of Kv2.1 Channels to the Axon Initial Segment by a Novel Nonconventional Secretory Pathway.2017

    • Author(s)
      Jensen CS, Watanabe S, Stas JI, Klaphaak J, Yamane A, Schmitt N, Olesen SP, Trimmer JS, Rasmussen HB, Misonou H.
    • Journal Title

      Journal of Neuroscience

      Volume: 37 Issue: 48 Pages: 11523-11536

    • DOI

      10.1523/jneurosci.3510-16.2017

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] タウタンパク質の神経細胞における軸索局在化機構と細胞内動態2018

    • Author(s)
      岩田実里、渡辺祥司、宮坂知宏、御園生裕明
    • Organizer
      認知症学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Study of axonal localization of tau in neurons2018

    • Author(s)
      Minori Iwata, Shoji Watanabe, Tomohiro Miyasaka, Hiroaki Misonou
    • Organizer
      Society for Neuroscience meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Neurobiologi of the axon2016

    • Author(s)
      御園生裕明
    • Organizer
      OIST symposium
    • Place of Presentation
      OIST、沖縄
    • Year and Date
      2016-09-25
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited

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Published: 2016-04-21   Modified: 2020-03-30  

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