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Molecular mechanisms that control the functional localization of the striatum

Research Project

Project/Area Number 16K07031
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Nerve anatomy/Neuropathology
Research InstitutionJichi Medical University

Principal Investigator

Takahashi Masanori  自治医科大学, 医学部, 准教授 (20361074)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords線条体 / 領域化 / 神経回路 / トレーサー / 神経科学 / 解剖学
Outline of Final Research Achievements

In this study, I analyzed Cdh20 expression patterns and CDH20 protein localization in the basal ganglia circuit in rats. Cdh20 was expressed in layer 5 PT-type cortical projection neurons, midbrain dopamine neurons, and external globus pallidus neurons, but not in thalamic parafascicular nucleus neurons and midbrain reticular neurons. In the striatum, the number of CDH20-positive cells was lower than in Cdh20 expressing cells. In addition, we constructed shRNA AAV vectors for Cdh20 knockdown and obtained high titer virus.

Academic Significance and Societal Importance of the Research Achievements

背外側線条体には、身体の各部位が脳の領域に対応する「体部位局在地図」が存在し、後肢、前肢、および舌などの運動機能が異なるサブドメインにおいて制御されている。しかしながら、線条体サブドメイン形成や機能局在化を制御する分子機構は未解明である。本研究で明らかにした大脳基底核におけるCdh20発現やタンパク質局在様式からCdh20が線条体サブドメイン形成や神経回路形成に関わることが示唆される。また本研究で構築したCdh20 shRNA AAVベクターを用いることで、生体内でのCdh20の機能を明らかにすることができ、複雑な随意運動の制御機構の一端を解明できる可能性が考えられる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (10 results)

All 2018 2017 2016 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (5 results) Remarks (1 results)

  • [Journal Article] Conserved and divergent functions of Pax6 underlie species-specific neurogenic patterns in the developing amniote brain2018

    • Author(s)
      Yamashita Wataru、Takahashi Masanori、Kikkawa Takako、Gotoh Hitoshi、Osumi Noriko、Ono Katsuhiko、Nomura Tadashi
    • Journal Title

      Development

      Volume: 145 Issue: 8 Pages: 159764-159764

    • DOI

      10.1242/dev.159764

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mice doubly deficient in Six4 and Six5 show ventral body wall defects reproducing human omphalocele.2018

    • Author(s)
      Takahashi M., Tamura M., Sato S. and Kawakami K
    • Journal Title

      Disease Models & Mechanisms

      Volume: 11 Issue: 10 Pages: 1-14

    • DOI

      10.1242/dmm.034611

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Electroporation in the Rodent Embryonic Brain Using Whole Embryo Culture System.2017

    • Author(s)
      Kikkawa T, Takahashi M, Osumi N
    • Journal Title

      Curr Protoc Neurosci.

      Volume: 78 Issue: 1 Pages: 1-16

    • DOI

      10.1002/cpns.21

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Six1 is required for mouse dental follicle cell and human periodontal ligament-derived cell proliferation.2016

    • Author(s)
      Kawasaki T, Takahashi M, Yajima H, Mori Y, Kawakami K.
    • Journal Title

      Dev Growth Differ.

      Volume: 58 Issue: 12 Pages: 530-545

    • DOI

      10.1111/pin.12471

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Six1 regulates Six1 regulates initial knot formation and lingual-labial asymmetry in incisor development.2018

    • Author(s)
      Takahashi, M. and Kawakami, K.
    • Organizer
      第51回日本発生生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Six4/Six5二重遺伝子変異マウスを用いた臍帯ヘルニア発症機序の解析2018

    • Author(s)
      高橋将文、田村勝、川上潔
    • Organizer
      第123回日本解剖学会総会・全国学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] Six1 regulates growth of dental papilla and lingual-labial asymmetry in the developing mandibular incisor.2017

    • Author(s)
      Takahashi, M. and Kawakami, K.
    • Organizer
      第50回日本発生生物学会年会
    • Related Report
      2017 Research-status Report
  • [Presentation] Mice defective of Six4 and Six5, show abnormal growth and epithelialization of the primary body wall, resulting in omphalocele with no severe defects in abdominal muscle differentiation.2016

    • Author(s)
      Takahashi, M. Tamura, M. and Kawakami, K.
    • Organizer
      第39回日本分子生物学会年
    • Place of Presentation
      パシフィコ横浜(神奈川県、横浜市)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report
  • [Presentation] Six4 and Six5 are required for ventral body wall closure and morphogenesis of the primary body wall.2016

    • Author(s)
      Takahashi, M. and Kawakami
    • Organizer
      JSDB Special Symposium: Frontier of Developmental Biology
    • Place of Presentation
      東京大学小柴ホール(東京都文京区)
    • Year and Date
      2016-06-02
    • Related Report
      2016 Research-status Report
  • [Remarks] 自治医科大学 分子病態治療研究センター 細胞生物研究部

    • URL

      http://www.jichi.ac.jp/biol/home.html

    • Related Report
      2018 Annual Research Report

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Published: 2016-04-21   Modified: 2020-03-30  

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