Construction of a mouse template brain with variable annotation numbers from 1 to 860

• Project/Area Number: 16K07032
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: Keio University
• Principal Investigator: Takata Norio 慶應義塾大学, 医学部(信濃町), 特任講師 (50415212)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 標準脳 / テンプレート / Allen / brain / 関心領域 / MRI / fMRI / mouse / マウス / 脳 / アレン / ROI / BOLD / 解剖学的階層性

Outline of Final Research Achievements

In magnetic resonance imaging (MRI) of the human brain, there is a standard brain in which brain region names correspond to brain coordinates. However, there is no practical one in the mouse brain. Region of interest (ROI) has been arbitrary and reproduction of other researchers' ROIs were difficult. Allen Institute released 3D-reconstruction data of the mouse brain in 2015. This study aimed to make it available for MRI analysis. As a result, we constructed a flexible template of the mouse brain. The total number of brain regions is variable according to the research purpose. New ROIs can be prepared using gene expression and neuro-projection data provided by the Allen Institute. Sharing among researchers is straightforward.

Academic Significance and Societal Importance of the Research Achievements

MRIを用いた脳研究では標準脳の整備が重要である。被験者ごとに異なる形態を示す脳の活動を比較するために用いるからである。ヒト脳ではこれが整備されているが、マウス脳では存在しなかった。本研究は脳部位数を柔軟に変更できるマウス標準脳の作成方法を構築した。研究目的ごとに最適な脳部位数を備えるマウス標準脳が利用可能となった。

Research Products

• [Journal Article] Optogenetic astrocyte activation evokes BOLD fMRI response with oxygen consumption without neuronal activity modulation (2018)
  • Author(s): Norio Takata, Yuki Sugiura, Keitaro Yoshida, Miwako Koizumi, Hiroshi Nishida, Kurara Honda, Ryutaro Yano, Yuji Komaki, Ko Matsui, Makoto Suematsu , Masaru Mimura, Hideyuki Okano, Kenji F. Tanaka
  • Journal Title: Glia Volume: 印刷中 Issue: 9 Pages: 2013-2023
  • DOI: 10.1002/glia.23454
  • Peer Reviewed
• [Journal Article] Visualization of myelinated fiber bundles orientation during brain slice preparation by reflection polarized light microscopy (2018)
  • Author(s): Takata Norio、Kusayanagi Kohei、Maruyama Hironori、Eitai Kenji、Abo Masaaki、Akiyama Fumihiko、Tajima Ryo、Inaba Shinsuke、Tanaka Kenji F.、Mimura Masaru、Sanaka Kaoru
  • Journal Title: Microscopy Research and Technique Volume: 81 Issue: 12 Pages: 1366-1373
  • DOI: 10.1002/jemt.23077
  • Peer Reviewed
• [Journal Article] Ventrolateral striatal medium spiny neurons positively regulate food-incentive, goal-directed behavior independently of D1 and D2 selectivity. (2017)
  • Author(s): Akiyo Natsubori, Iku Tsustui-Kimura, Hiroshi Nishida, Youcef Bouchekioua, Hiroshi Sekiya, Motokazu Uchigashima, Masahiko Watanabe, Alban de Kerchove d'Exaerde, Masaru Mimura, Norio Takata, and Kenji Tanaka
  • Journal Title: Journal of Neuroscience Volume: 37 Issue: 10 Pages: 2723-2733
  • DOI: 10.1523/jneurosci.3377-16.2017
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Dysfunction of ventrolateral striatal dopamine receptor type 2-expressing medium spiny neurons impairs instrumental motivation. (2017)
  • Author(s): Tsutsui-Kimura I, Takiue H, Yoshida K, Xu M, Yano R, Ohta H, Nishida H, Bouchekioua Y, Okano H, Uchigashima M, Watanabe M, Takata N, Drew MR, Sano H, Mimura M, Tanaka KF.
  • Journal Title: Nat Commun. Volume: 8 Issue: 1 Pages: 14304-14304
  • DOI: 10.1038/ncomms14304
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Physiological effects of a habituation procedure for functional MRI in awake mice using a cryogenic radiofrequency probe (2016)
  • Author(s): Keitaro Yoshida, Yu Mimura, Ryosuke Ishihara, Hiroshi Nishida, Yuji Komaki, Tomohito Minakuchi, Tomokazu Tsurugizawa, Masaru Mimura, Hideyuki Okano, Kenji F. Tanaka, Norio Takata
  • Journal Title: J Neurosci Methods. Volume: 274 Pages: 38-48
  • DOI: 10.1016/j.jneumeth.2016.09.013
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 視床を抑制する視床網様核の新規振動活動は安静時の全脳活動を規定する (2019)
  • Author(s): 高田則雄
  • Organizer: 第42回日本神経科学会
  • Int'l Joint Research / Invited
• [Presentation] Causal evidence of BOLD-fMRI signal generation by astrocytes without neuronal activation (2019)
  • Author(s): 高田則雄
  • Organizer: The 2nd FRIS-TFC Joint Symposium: Unlocking the Brain from Engineering Approaches
  • Int'l Joint Research / Invited
• [Presentation] 視床を抑制する視床網様核の集合Ca2+活動は新規発振現象を示す (2018)
  • Author(s): 高田則雄、夏堀晃世、三村將、田中謙二
  • Organizer: 第41回日本神経科学会
• [Presentation] 光遺伝学的fMRIを用いた海馬活動伝播様式の全脳解析 (2017)
  • Author(s): 高田則雄
  • Organizer: 次世代プロジェクト「異なる時期の虚血が脳の発達に及ぼす影響の多層的アプローチによる解明と治療戦略構築」国際シンポジウム
  • Int'l Joint Research / Invited
• [Presentation] Dissection of downstream targets of the hippocampus using optogenetic fMRI (2017)
  • Author(s): 高田則雄
  • Organizer: 次世代プロジェクト「異なる時期の虚血が脳の発達に及ぼす影響の多層的アプローチによる解明と治療戦略構築」国際シンポジウム
  • Place of Presentation: 慶応義塾大学医学部
  • Int'l Joint Research / Invited