Effects of a DAP12-mediated signal on microglial activation after injury
| Project/Area Number |
16K07055
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | グリア / ミクログリア / 神経炎症 / 疼痛 / アロディニア / ミクログリアマーカー / TREM2 / DAP12 / 炎症 / マーカー / 神経損傷 / サイトカイン / 再生医学 / 神経障害性疼痛 |
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| Outline of Final Research Achievements |
DAP12 is considered to work as a signal transduction chain of some ligand-binding receptors in microglia. Our study, using allodynia model of mice, revealed that DAP12 can regulate microglial activity both positively and negatively after injury. When DAP12 makes complex with TREM2, TREM2/DAP12 promotes microglial pro-inflammatory responses after injury, whereas Siglec-H/DAP12 complex suppresses. Furthermore, we demonstrated Siglec-H as a microglia-specific marker that discriminates microglia from brain macrophages and CNS-infiltrating monocytes.
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| Academic Significance and Societal Importance of the Research Achievements |
シグナル伝達鎖DAP12は、異なるリガンド認識鎖（TREM2またはSiglec-H）と複合体を形成することにより、ミクログリアの活性化を正/負両方に制御することを見出した。DAP12を介するシグナルの適切な制御により、神経損傷後のミクログリアの性質を神経保護的に転換できる可能性が期待される。また、本研究により示したSiglec-Hの発現特異性は、今後のミクログリア研究において有益な情報になると考えられる。
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Report
(4 results)
Research Products
(33 results)
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[Journal Article] Branched Sialylated <i>N</i>-glycans Are Accumulated in Brain Synaptosomes and Interact with Siglec-H2018
Author(s)
M. Handa-Narumi, T. Yoshimura, H. Konishi, Y. Fukata, Y. Manabe, K. Tanaka, G.-m. Bao, H. Kiyama, K. Fukase, K. Ikenaka
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Journal Title
Cell Structure and Function
Volume: 43
Issue: 2
Pages: 141-152
DOI
NAID
ISSN
0386-7196, 1347-3700
Related Report
Peer Reviewed / Open Access
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[Journal Article] Exposure to diphtheria toxin during the juvenile period impairs both inner and outer hair cells in C57BL/6 mice2017
Author(s)
Konishi H, Ohgami N, Matsushita A, Kondo Y, Aoyama Y, Kobayashi M, Nagai T, Ugawa S, Yamada K, Kato M, Kiyama H
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Journal Title
Neuroscience
Volume: 印刷中
Pages: 15-23
DOI
NAID
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] R-spondin 2 promotes acetylcholine receptor clustering at the neuromuscular junction via Lgr5.2016
Author(s)
Nakashima H., Ohkawara B., Ishigaki S., Fukudome T., Ito K., Tsushima M., Konishi H., Okuno T., Yoshimura T., Ito M., Masuda A., Sobue G., Kiyama H., Ishiguro N., Ohno K.
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Journal Title
Sci Rep
Volume: 22
Issue: 1
Pages: 28512-28512
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Alteration of phospholipid localization induces microglial ramification2016
Author(s)
Tokizane K, Konishi H, Makide K, Kawana H, Nakamuta S, Kaibuchi K, Ohwada T, Aoki J, Kiyama H
Organizer
Cold Spring Harbor Asia Conferences, New insights into Glia function & Dysfunction
Place of Presentation
World Hotel Grand Dushulake Suzhou (Suzhou, China)
Related Report
Int'l Joint Research
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