| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
The role of fragile X mental retardation protein (FMRP) in axon guidance and synapse formation during neural circuit formation was examined in relation to ubiquitination and local translation. Our results suggest that after stimulation with the axon guidance molecule Sema3A, FMRP was degraded in growth cones by the ubiquitin-proteasome system, followed by that local translation was triggered to induce a morphological change of growth cones. We also identified the ubiquitination related gene for FMRP, and found that the gene product induced disappearance of FMRP after granular accumulation of FMRP. FMRP-containing granules were also observed during synapse formation. Therefore, our findings suggested that ubiquitination of FMRP is involved in neural circuit formation through regulation of granule formation and local translation of FMRP.
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