Signaling interaction between two G protein-coupled receptors of the same subclass

• Project/Area Number: 16K07063
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Saitama Medical University
• Principal Investigator: Suo Satoshi 埼玉医科大学, 医学部, 講師 (20596845)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 神経伝達物質 / Gタンパク質共役型受容体 / C. elegans / カルシウムイオン / カルシウム / cAMP / 受容体 / 線虫 / ドーパミン / オクトパミン / 神経科学 / 薬理学 / 遺伝学

Outline of Final Research Achievements

G protein-coupled receptors are receptors for various biomolecules including neurotransmitters. In many cases, there are multiple receptors with homologous structures that binds to the same neurotransmitter. In C. elegans, the neurotransmitter octopamine induces CREB activation in the SIA neurons. In this study, we found that, unlike the CREB activation which require both octopamine receptor SER-3 and SER-6, the octopamine-dependent calcium response was induced in SER-3 or SER-6 single mutants and the calcium response was diminished only in the mutants lacking all octopamine receptors, suggesting that the receptor requirements are different between the CREB activation and calcium response.

Academic Significance and Societal Importance of the Research Achievements

一つの伝達物質に対し似た構造を持つGタンパク質共役型受容体が複数存在するが、その意義は不明な点が多い。本研究では、シグナル伝達ごとに必要となる受容体の組合せが異なることを明らかにし、複数の受容体がより複雑なシグナル伝達を生み出していることを示唆することで、Gタンパク質共役型受容体によるシグナル伝達の解明に貢献した。Gタンパク質共役型受容体は様々な薬の標的であることから、薬の作用メカニズムの解明や新薬の開発に貢献すると考えられる。

Research Products

• [Journal Article] Sexually Dimorphic Regulation of Behavioral States by Dopamine in Caenorhabditis elegans (2019)
  • Author(s): Suo Satoshi、Harada Kazuki、Matsuda Shogo、Kyo Koki、Wang Min、Maruyama Kei、Awaji Takeo、Tsuboi Takashi
  • Journal Title: The Journal of Neuroscience Volume: 39 Issue: 24 Pages: 4668-4683
  • DOI: 10.1523/jneurosci.2985-18.2019
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] RGB-Color Intensiometric Indicators to Visualize Spatiotemporal Dynamics of ATP in Single Cells (2018)
  • Author(s): Arai Satoshi、Kriszt R?kus、Harada Kazuki、Looi Liang-Sheng、Matsuda Shogo、Wongso Devina、Suo Satoshi、Ishiura Shoichi、Tseng Yu-Hua、Raghunath Michael、Ito Toshiro、Tsuboi Takashi、Kitaguchi Tetsuya
  • Journal Title: Angewandte Chemie International Edition Volume: 57 Issue: 34 Pages: 10873-10878
  • DOI: 10.1002/anie.201804304
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Regulation of body size in Caenorhabditis elegans: effects of environmental factors and the nervous system (2017)
  • Author(s): Takashi Nagashima, Shoichi Ishiura and Satoshi Suo
  • Journal Title: International Journal of Developmental Biology Volume: 61 Issue: 6-7 Pages: 367-374
  • DOI: 10.1387/ijdb.160352ss
  • Peer Reviewed
• [Journal Article] Regulation of body size in Caenorhabditis elegans: effects of environmental factors and the nervous system (2017)
  • Author(s): Takashi Nagashima, Shoichi Ishiura, Satoshi Suo
  • Journal Title: The International Journal of Developmental Biology Volume: 印刷中
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Sexually dimorphic dopaminergic signaling regulates behavioral states of C. elegans (2019)
  • Author(s): Suo, S., Harada, K., Matsuda, S., Kyo, K., Wang, M., Maruyama, K., Awaji, T., Tsuboi, T.
  • Organizer: International C. elegans Meeting
  • Int'l Joint Research
• [Presentation] ドーパミンは線虫C. elegansの運動量の性差を生み出す (2019)
  • Author(s): 周防 諭、原田 一貴、松田 翔吾、姜興起、王 旻、丸山 敬、淡路 健雄、坪井 貴司
  • Organizer: Neuro2019 日本神経科学会大会・日本神経化学会大会
• [Presentation] Dopamine increases locomotor activity in C. elegans males (2018)
  • Author(s): Satoshi Suo, Kazuki Harada, Shogo Matsuda, Takashi Tsuboi
  • Organizer: Asia-Pacific C. elegans Meeting
  • Int'l Joint Research
• [Presentation] ドーパミンはC. elegansの自発運動の性差を作り出す (2018)
  • Author(s): 周防諭
  • Organizer: 線虫研究の未来を創る会
• [Presentation] ドーパミンはC. elegansオスの自発運動量を制御する (2018)
  • Author(s): 周防 諭、原田 一貴、松田 翔吾、王 旻、丸山 敬、淡路 健雄、坪井 貴司
  • Organizer: 分子生物学会年会
• [Presentation] C. elegansにおいてドーパミンは体のサイズと産卵を制御する (2016)
  • Author(s): 永嶋 宇、大網 栄太郎、石浦 章一、周防 諭
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜、横浜
  • Year and Date: 2016-11-30
• [Presentation] Dopamine and octopamine regulate body size of C. elegans. (2016)
  • Author(s): 周防 諭、永嶋 宇、大網 栄太郎、石浦 章一
  • Organizer: CeNeuro 2016
  • Place of Presentation: 名古屋大学、名古屋
  • Year and Date: 2016-07-27
  • Int'l Joint Research
• [Remarks] 埼玉医科大学薬理学教室
  • URL: http://www.saitama-med.ac.jp/uinfo/yakuri/index.html
• [Remarks] 所属機関ホームページ
  • URL: http://www.saitama-med.ac.jp/uinfo/yakuri/suo.html
• [Remarks] 埼玉医科大学 薬理学教室
  • URL: http://www.saitama-med.ac.jp/uinfo/yakuri/suo.html
• [Remarks] 研究内容の紹介
  • URL: http://www.saitama-med.ac.jp/uinfo/yakuri