• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The Effects of Astrocyte and Oligodendrocyte Lineage Cell Interaction on White Matter Injury under Chronic Cerebral Hypoperfusion

Research Project

Project/Area Number 16K07067
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Neurochemistry/Neuropharmacology
Research InstitutionJuntendo University

Principal Investigator

Miyamoto Nobukazu  順天堂大学, 医学部, 准教授 (10365661)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordscell-cell interaction / oligodendrogenesis / BDNF / 慢性脳虚血低潅流 / cell cell interaction / 慢性低潅流モデル / S100β / 脳血管性認知症 / Astro-Oligo niche / 脳虚血性白質障害保護 / オリゴデンドロサイト / アストロサイト / 細胞間interaction
Outline of Final Research Achievements

We examined the effects of the interaction between astrocyte and oligodendrocyte lineage cells on myelination regarding the mechanism of impairment. White matter injury progressed in the BCAS model as myelin decreased. The numbers of OPCs and astrocytes increased after the operation, whereas that of OLGs decreased at day 28. BDNF continuously decreased until day 28. Differentiation was disrupted under the stressed conditions in the cell culture, but improved after administration of astrocyte-conditioned medium containing BDNF. Astrocytes with BDNF underwent differentiation, but differentiation was impaired under the stressed conditions due to the reduction of BDNF. We examined S100B regarding the mechanism of impairment. S100B is mainly expressed by mature astrocytes. GFAP-positive astrocytes increased in the corpus callosum in the BCAS model, whereas the number of mature astrocytes continued to decrease, resulting in reduced BDNF.

Academic Significance and Societal Importance of the Research Achievements

オリゴデンドロサイトの再生に関しては多発性硬化症研究の分野では報告があるものの,虚血性白質障害の分野においての報告はいまだ数少ない.我々が考案した本研究は,脳虚血領域の中でも慢性期の病態に焦点を絞ることができ,さらにはいまだ有効な治療方法のない慢性期虚血性白質障害の治療に一石と投じれると考えられる.また、その役割機能を解明することは今日,社会的問題にもなっているアルツハイマー型認知症の克服にも発展的臨床応用が可能になると考える.BDNFを代表とするアストロサイトからの栄養因子強化は,白質保護につながるだけではなく,血管性認知症の治療ターゲットになる可能性が示せた.

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (4 results)

All 2019 2017 2016

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results) Presentation (3 results)

  • [Journal Article] The Effects of Astrocyte and Oligodendrocyte Lineage Cell Interaction on White Matter Injury under Chronic Cerebral Hypoperfusion2019

    • Author(s)
      Magami Shunsuke、Miyamoto Nobukazu、Ueno Yuji、Hira Kenichiro、Tanaka Ryota、Yamashiro Kazuo、Oishi Hidenori、Arai Hajime、Urabe Takao、Hattori Nobutaka
    • Journal Title

      Neuroscience

      Volume: 406 Pages: 167-175

    • DOI

      10.1016/j.neuroscience.2019.03.004

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 慢性脳虚血による脳白質障害とBrain Derived Neurotrophic Factor (BDNF)の白質保護作用について2019

    • Author(s)
      眞上俊亮,宮元伸和,大石英則,服部信孝,新井一
    • Organizer
      第43回日本脳卒中学会学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 慢性脳虚血による脳白質障害とBrain Derived Neurotrophic Factor (BDNF)の白質保護作用について2017

    • Author(s)
      眞上俊亮,宮元伸和,大石英則,服部信孝,新井一
    • Organizer
      第43回日本脳卒中学会学術総会
    • Related Report
      2017 Research-status Report
  • [Presentation] オリゴデンドロサイト新生に対するAKAP12の重要性2016

    • Author(s)
      宮元伸和,眞木崇州,Ji Hae Seo,田中亮太,Irwin H. Gelman,Eng H. Lo,荒井健,卜部貴夫,服部信孝
    • Organizer
      第59回日本脳循環代謝学会学術集会
    • Place of Presentation
      徳島市 あわぎんホール
    • Year and Date
      2016-11-11
    • Related Report
      2016 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi