| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Synaptotagmin (Syt) is known to be the calcium sensor in presynaptic terminals. I have carried out my project to examine conformation changes of Syt in vivo using the genetic code expansion method. This method enables to incorporate the fluorescent unnatural amino acid (Anap) in the polypeptide of target protein as a fluorescent marker for conformation changes. First, I established the technique to apply the method in zebrafish embryo. Second, I searched the position into which Anap is incorporated using acethylcholine receptors and showed that only one site is available for the measurement of Anap fluorescence change. This means that limited positions can be used as an incorporation site to probe conformation change. These are big steps for the measurement of protein structure changes in living animals.
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