A regulatory role of the double-stranded RNA editing enzyme ADAR1 for adaptive immune response
| Project/Area Number |
16K07082
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Chiba University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
有馬 雅史 獨協医科大学, 医学部, 准教授 (00202763)
幡野 雅彦 千葉大学, 大学院医学研究院, 教授 (20208523)
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| Project Period (FY) |
2016-10-21 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 免疫応答 / 疾患モデル |
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| Outline of Final Research Achievements |
In order to elucidate the role of the double-stranded RNA editing enzyme ADAR1 (adenosine deaminase acting on RNA-1) in adaptive immunity, we have analyzed mice deficient in ADAR1 specifically for activated T cells or B cells. In both activated T-cell and B-cell-specific ADAR1-deficient mice, differentiation of Germinal Center (GC) -Tfh cells and GC-B cells after immunization with NP-CG / Alum or OVA / Alum was suppressed, and high affinity IgG antibody and OVA-specific IgE antibody were significantly reduced. These results indicate that ADAR1 plays an extremely important role in the adaptive immune system by acting endogenously on T cells and B cells and by being involved in T-B cell interactions.
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| Academic Significance and Societal Importance of the Research Achievements |
免疫後のリンパ球の機能分化や機能制御における詳細なメカニズムは未だ明らかでない。我々は獲得免疫にける機能が明らかでないADAR1について注目し、本研究により特にGC-Tfh細胞やGC-B細胞の機能にADAR１が重要な役割を果たすことを見出した。本研究の成果は、今後、GC-Tfh細胞とGC－B細胞の分化や機能の制御機構の研究および免疫システムの解明研究において意義があると考えられる。また本研究の成果はアレルギー性疾患やHIVなど有効なワクチンが存在しない感染症に対する効果的なワクチン療法の開発へとつながる点に社会的に意義がある。
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Report
(5 results)
Research Products
(36 results)
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[Journal Article] Various associations of aging and long-term HIV infection with different neurocognitive functions: detailed analysis of a Japanese nationwide multicenter study2019
Author(s)
Kensuke Komatsu, Ei Kinai, Maiko Sakamoto, Toshibumi Taniguchi, Aya Nakao, Tatsuro Sakata, Akiko Iizuka, Teppei Koyama, Toki Ogata, Akihiro Inui, Shinichi Oka
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Journal Title
Journal of NeuroVirology
Volume: 25
Pages: 208-220
Related Report
Peer Reviewed
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[Journal Article] Allergic TH2 Response Governed by B-Cell Lymphoma 6 Function in Naturally Occurring Memory Phenotype CD4+ T Cells2018
Author(s)
Takashi Ogasawara,Yuko Kohashi,Jun Ikari,Toshibumi Taniguchi,Nobuhide Tsuruoka,Haruko Watanabe-Takano,Lisa Fujimura,Akemi Sakamoto,Masahiko Hatano,Hirokuni Hirata,Yasutsugu Fukushima,Takeshi Fukuda,Kazuhiro Kurasawa,Koichiro Tatsumi,Takeshi Tokuhisa,Masafumi Arima
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Journal Title
Frontiers in Immunology
Volume: 9
Pages: 750-750
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Development of chronic allergic responses by dampening Bcl6-mediated suppressor activity in memory T helper 2 cells.2017
Author(s)
Ogasawara T, Hatano M, Satake H, Ikari J, Taniguchi T, Tsuruoka N, Watanabe-Takano H, Fujimura L, Sakamoto A, Hirata H, Sugiyama K, Fukushima Y, Nakae S, Matsumoto K, Saito H, Fukuda T, Kurasawa K, Tatsumi K, Tokuhisa T, Arima M.
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Journal Title
Proc Natl Acad Sci U S A.
Volume: 114
Issue: 5
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] 千葉県臨床検査技師会が行っている休日街頭HIV抗体検査の実施状況について2017
Author(s)
布施 義也, 谷口俊文, 中山茂, 綿引一成, 吉田隆, 東和彦, 小野寺清隆, 大野光江, 三末高央, 小川優, 下条小百合, 小林健, 坂本浩輝
Organizer
日本エイズ学会
Related Report
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[Presentation] HIV関連神経認知障害(HAND)における神経心理検査別の関連因子に関する検討(J-HAND研究)(第2報)2017
Author(s)
小松賢亮, 木内英, 坂本麻衣子, 谷口俊文, 中尾綾, 坂田達朗, 飯塚暁子, 小山徹平, 緒方釈, 乾啓洋, 岡慎一, J-HAND StudyGroup
Organizer
日本エイズ学会
Related Report
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