| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Mouse embryonic stem cells (mESCs) have been established in conventional serum culture. mESCs derived from 129 mouse strain are stably self-renewing in serum culture. In contrast, mESCs from non-obese diabetic (NOD) mouse strain is not yet maintained in serum culture. In this project, we tried to explore why ESCs from 129 strain were stably established and continued to self-renew in serum culture. We identified transcription factor Tfcp21 which was specifically retained in 129-ESCs. Maintaining the expression of Tfcp21l in 2i-established NOD-ESCs allowed self-renewal in an inhibitor-free serum condition. Tfcp2l1 enhanced LIF-Stat3 activity in NOD-ESCs at a similar level to that in 129-ESCs. These results indicate that Tfcp2l1 supports 129-ESCs self-renewal in serum through enhancing LIF-Stat3.
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