| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
In this study, we show that cancer cells rapidly consume glutamine and secrete ammonia during the growth phase and that glutamine limitation suppresses cancer cell proliferation. Strikingly, this proliferation impairment can be overcome by supplementing cells with ammonia, depending on the cellular level of the enzyme glutamate dehydrogenase 2 (GDH2), which synthesizes glutamate from ammonia and α-ketoglutarate. Moreover, we demonstrate that GDH2 is sufficient for glutamate production to support proliferation or survival under glutamine-depleted conditions. Our findings provide insight into how cancer cells survive under glutamine deprivation conditions and contribute to the elucidation of cancer cell proliferation mechanisms.
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