• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of a cell-free DNA analysis system using a technology for high-fidelity sequencing and absolute quantitation of DNA molecules

Research Project

Project/Area Number 16K07157
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Tumor diagnostics
Research InstitutionNara Institute of Science and Technology (2017-2018)
Research Institute, Osaka Medical Center for Cancer and Cardiovascular Disaeses (2016)

Principal Investigator

KUKITA Yoji  奈良先端科学技術大学院大学, 先端科学技術研究科, 特任准教授 (60372744)

Research Collaborator KATO kikuya  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords血中遊離DNA / 血中循環腫瘍DNA / ALK融合遺伝子 / 癌 / 体細胞変異 / 腫瘍マーカー / 核酸
Outline of Final Research Achievements

To develop a system to analyze circulating tumor DNA from blood of pancreatic cancer and lung cancer patients, we improved the high-fidelity sequencing technology, NOIR-SeqS, and designed two gene panels targeting mutation hotspots of 8 to 9 cancer-related genes. Furthermore, we devised a bioinformatic variant filter to remove non-cancer specific mutations among the detected ones. The clinical usefulness of our analysis system was confirmed by analyzing blood samples from 143 pancreatic cancer patients. We also developed an analysis method specialized for the detecting ALK fusion gene mutations found in some lung cancer patients.

Academic Significance and Societal Importance of the Research Achievements

血中遊離DNAは微量かつ生体試料として低品質であるため、信頼できる解析結果を得るのは技術的に困難であった。本研究で開発された解析技術により、癌患者血液検体から癌細胞由来の遺伝子変異を高精度に検出することが可能となった。本法を用いた膵癌患者血液検体の解析から、血中循環腫瘍DNAの検出は治療中の病態把握や効果判定などに有用であることが示唆された。このことは膵癌などの難治癌患者が置かれている状況を確実に改善していくものである。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (14 results)

All 2019 2018 2017 2016

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Open Access: 6 results) Presentation (4 results) (of which Int'l Joint Research: 1 results) Book (2 results)

  • [Journal Article] Detection of circulating tumor DNA from pancreatic cancer using next-generation sequencing and possible clinical applications2018

    • Author(s)
      大川和良, 高田良司, 片山和宏, 久木田洋児, 加藤菊也
    • Journal Title

      Suizo

      Volume: 33 Issue: 6 Pages: 937-943

    • DOI

      10.2958/suizo.33.937

    • NAID

      130007569035

    • ISSN
      0913-0071, 1881-2805
    • Year and Date
      2018-12-25
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Selective identification of somatic mutations in pancreatic cancer cells through a combination of next-generation sequencing of plasma DNA using molecular barcodes and a bioinformatic variant filter2018

    • Author(s)
      Kukita Yoji、Ohkawa Kazuyoshi、Takada Ryoji、Uehara Hiroyuki、Katayama Kazuhiro、Kato Kikuya
    • Journal Title

      PLoS ONE

      Volume: 13 Issue: 2 Pages: e0192611-e0192611

    • DOI

      10.1371/journal.pone.0192611

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Monitoring of treatment responses and clonal evolution of tumor cells by circulating tumor DNA of heterogeneous mutant EGFR genes in lung cancer.2016

    • Author(s)
      Imamura F, Uchida J, Kukita Y, Kumagai T, Nishino K, Inoue T, Kimura M, Oba S, Kato K
    • Journal Title

      Lung Cancer

      Volume: 94 Pages: 68-73

    • DOI

      10.1016/j.lungcan.2016.01.023

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Numerical indices based on circulating tumor DNA for the evaluation of therapeutic response and disease progression in lung cancer patients.2016

    • Author(s)
      Kato K, Uchida J, Kukita Y, Kumagai T, Nishino K, Inoue T, Kimura M, Oba S, Imamura F
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 29093-29093

    • DOI

      10.1038/srep29093

    • NAID

      120006323742

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Characterization of the T cell receptor beta chain repertoire in tumor infiltrating lymphocytes2016

    • Author(s)
      Nakanishi, K., Kukita, Y., Segawa, H., Inoue, N., Ohue, M., Kato, K.
    • Journal Title

      Cancer Medicine

      Volume: 5 Issue: 9 Pages: 2513-2521

    • DOI

      10.1002/cam4.828

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Early responses of EGFR circulating tumor DNA to EGFR tyrosine kinase inhibitors in lung cancer treatment.2016

    • Author(s)
      Imamura F, Uchida J, Kukita Y, Kumagai T, Nishino K, Inoue T, Kimura M, Kato K
    • Journal Title

      Oncotarget

      Volume: 7 Issue: 44 Pages: 71782-71789

    • DOI

      10.18632/oncotarget.12373

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Homozygous inactivation of CHEK2 is linked to a familial case of multiple primary lung cancer with accompanying cancers in other organs.2016

    • Author(s)
      Kukita Y, Okami J, Yoneda-Kato N, Nakamae I, Kawabata T, Higashiyama M, Kato J, Kodama K, Kato K
    • Journal Title

      Cold Spring Harbor Molecular Case Studies

      Volume: 2 Issue: 6 Pages: a001032-a001032

    • DOI

      10.1101/mcs.a001032

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Transient appearance of circulating tumor DNA associated with de novo treatment.2016

    • Author(s)
      Kato K, Uchida J, Kukita Y, Kumagai T, Nishino K, Inoue T, Kimura M, Imamura F
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 38639-38639

    • DOI

      10.1038/srep38639

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Selective detection of somatic mutations in circulating tumor DNA from pancreatic cancer patients by next-generation sequencing and a bioinformatic variant filter2018

    • Author(s)
      Kukita Y, Takada R, Okawa K, Katayama K, Kato K
    • Organizer
      Human Genome Meeting 2018
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Selective detection of somatic mutations in circulating tumor DNA from cancer patients2017

    • Author(s)
      久木田洋児, 高田良司, 大川和良, 片山和宏, 加藤菊也
    • Organizer
      第76回日本癌学会学術総会
    • Related Report
      2017 Research-status Report
  • [Presentation] DNA分子高精度配列決定及び絶対定量による血中遊離DNA中の突然変異検出2016

    • Author(s)
      久木田洋児
    • Organizer
      NGS現場の会 血液核酸マーカー分科会 第2回研究会
    • Place of Presentation
      東京
    • Related Report
      2016 Research-status Report
  • [Presentation] Non-overlapping integrated reads sequencing system for de novo detection of mutations in circulating tumor DNA(NOIRシーケンスシステムよる癌患者由来血漿DNA中のde novo突然変異検出)2016

    • Author(s)
      久木田洋児, 内田純二, 高田良司, 今村文生, 片山和宏, 加藤菊也
    • Organizer
      第75回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Related Report
      2016 Research-status Report
  • [Book] バイオイノベーションに向けて~バイオテクノロジーの新技術からの新しい視点~2019

    • Author(s)
      植田充美, 城口克之, 小川泰策, 笹川洋平, 二階堂愛, 久木田洋児, 他
    • Total Pages
      258
    • Publisher
      シーエムシー出版
    • ISBN
      9784781314112
    • Related Report
      2018 Annual Research Report
  • [Book] リキッドバイオプシー―体液中腫瘍マーカーの検出・解析技術―2017

    • Author(s)
      落谷孝広、前川真人、洪泰浩、加藤菊也、田原栄俊、前田瑞夫、細川和生、北森武彦、一木隆範、馬渡和真、今井靖、黒田雅彦、村上善基、湯川博、菊池春人、戸塚実、丹羽修、植松美幸、野村祐介、久木田洋児、他
    • Total Pages
      288
    • Publisher
      シーエムシー出版
    • ISBN
      9784781312590
    • Related Report
      2017 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi