| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
To analyze a comprehensive overview of intracellular phosphorylation networks, we constructed phosphorylation model of individual human protein kinase based on in vitro kinase-substrate relationships, and developed a method for prediction of physiological kinase substrates.
The predicted kinase-substrate relationships were evaluated by using a large-scale quantitative phosphoproteome profiling, in which cultured cells were treated with or without a kinase inhibitor. Responsible kinases for the downregulated phosphorylation sites were predicted using the our method. As a result, we successfully predicted inhibition of targeted and its downstream kinases and found that the prediction accuracy is higher than that of the existing techniques.
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