Generation of homoplasmic mtDNA modified cell library
| Project/Area Number |
16K07207
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Genome biology
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Wada Ken-Ichi 国立研究開発法人理化学研究所, 開拓研究本部, 協力研究員 (20525919)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | mtDNA / マイクロデバイス / ミトコンドリアゲノム改変 / 細胞質移植 / マイクロ流体デバイス / ミトコンドリア移植 / ミトコンドリアゲノム |
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| Outline of Final Research Achievements |
Single mitochondrion transfer is a possible approach for homoplasmic mtDNA modification. This study aimed to develop a novel method for single mitochondrion transfer and following mtDNA cloning to achieve homoplasmic mtDNA modification. In this study, I succeeded in performing single mitochondrion transfer between live single cells by achieving cell fusion through a 10 μm-length microtunnel using a microfluidic device, and in harvest of mitochondria transferred mtDNA-less cell from the microfluidic device. This provides an important basis for development of homoplasmic mtDNA modification method.
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| Academic Significance and Societal Importance of the Research Achievements |
実用的なmtゲノム改変技術は開発されていないため、mtゲノムの機能解析の実施には大きな制約がある。ホモプラズミックなmtゲノム改変の実現と、それに引きつづいてmtゲノム改変細胞ライブラリーを構築することによって、これまで制約の多かったmtゲノム解析を強力に推進する研究プラットフォームの創出が期待される。
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Report
(4 results)
Research Products
(12 results)
