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Generation of homoplasmic mtDNA modified cell library

Research Project

Project/Area Number 16K07207
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Genome biology
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Wada Ken-Ichi  国立研究開発法人理化学研究所, 開拓研究本部, 協力研究員 (20525919)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
KeywordsmtDNA / マイクロデバイス / ミトコンドリアゲノム改変 / 細胞質移植 / マイクロ流体デバイス / ミトコンドリア移植 / ミトコンドリアゲノム
Outline of Final Research Achievements

Single mitochondrion transfer is a possible approach for homoplasmic mtDNA modification. This study aimed to develop a novel method for single mitochondrion transfer and following mtDNA cloning to achieve homoplasmic mtDNA modification. In this study, I succeeded in performing single mitochondrion transfer between live single cells by achieving cell fusion through a 10 μm-length microtunnel using a microfluidic device, and in harvest of mitochondria transferred mtDNA-less cell from the microfluidic device. This provides an important basis for development of homoplasmic mtDNA modification method.

Academic Significance and Societal Importance of the Research Achievements

実用的なmtゲノム改変技術は開発されていないため、mtゲノムの機能解析の実施には大きな制約がある。ホモプラズミックなmtゲノム改変の実現と、それに引きつづいてmtゲノム改変細胞ライブラリーを構築することによって、これまで制約の多かったmtゲノム解析を強力に推進する研究プラットフォームの創出が期待される。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (12 results)

All 2019 2018 2017 2016

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (10 results) (of which Int'l Joint Research: 5 results) Book (1 results)

  • [Journal Article] Quantitative control of mitochondria transfer between live single cells using a microfluidic device2017

    • Author(s)
      Ken-Ichi Wada, Kazuo Hosokawa, Yoshihiro Ito, Mizuo Maeda
    • Journal Title

      Biology Open

      Volume: 6 Pages: 1960-1965

    • DOI

      10.1242/bio.024869

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] A novel method for quantitative organelles transfer using a microfluidic device2019

    • Author(s)
      Ken-Ichi Wada, Kazuo Hosokawa, Yoshihiro Ito, Mizuo Maeda
    • Organizer
      3rd International Symposium on Nanoarchitectonics for Mechanobiology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マイクロデバイスを用いたオルガネラ移植の量的制御2018

    • Author(s)
      和田健一,細川和生,伊藤嘉浩,前田瑞夫
    • Organizer
      第28回日本MRS年次大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] シングルセル解析を目指したマイクロ流体デバイスを用いたオルガネラ組成の操作2018

    • Author(s)
      和田健一,細川和生,伊藤嘉浩,前田瑞夫
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Quantitative control of mitochondria transfer between live single cells using a microfluidic device toward mtDNA editing2018

    • Author(s)
      和田健一,細川和生,伊藤嘉浩,前田瑞夫
    • Organizer
      第70回日本細胞生物学会 第51回日本発生生物学会合同大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Cytoplasmic transfer between live single cells by achieving transient cytoplasmic connection2017

    • Author(s)
      Ken-Ichi Wada, Kazuo Hosokawa, Yoshihiro Ito, Mizuo Maeda
    • Organizer
      The 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2017)
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Quantitative Control of Mitochondria Transfer Using a Microfluidic Device2017

    • Author(s)
      Ken-Ichi Wada, Kazuo Hosokawa, Yoshihiro Ito, Mizuo Maeda
    • Organizer
      The 15th International Conference on Advanced Materials (IUMRS-ICAM 2017)
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] マイクロデバイスを用いて直接的なミトコンドリア移植の量的制御を実現する2017

    • Author(s)
      和田健一、細川和生、伊藤嘉浩、前田瑞夫
    • Organizer
      第26回インテリジェント材料シンポジウム
    • Place of Presentation
      TWIns(東京都新宿区)
    • Related Report
      2016 Research-status Report
  • [Presentation] 直接的な細胞質移植を実現するマイクロ流体デバイスを用いた新規単一細胞操作技術2016

    • Author(s)
      和田健一、細川和生、伊藤嘉浩、前田瑞夫
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report
  • [Presentation] Quantitative control of mitochondria transfer between live single-cells by microfluidic device2016

    • Author(s)
      Ken-Ichi Wada, Kazuo Hosokawa, Yoshihiro Ito, Mizuo Maeda
    • Organizer
      International Conference on Single Cell Research 2016 Joint Conference of the 10th International Workshop on Approaches to Single-Cell Analysis & IIRC Conference
    • Place of Presentation
      Ito International Research Center (Bunkyo-ku, Tokyo)
    • Year and Date
      2016-11-16
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Controlled organelle transfer between live single cells by using microfluidic device2016

    • Author(s)
      Ken-Ichi Wada, Kazuo Hosokawa, Yoshihiro Ito, Mizuo Maeda
    • Organizer
      The 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)
    • Place of Presentation
      Dublin, Ireland
    • Year and Date
      2016-10-09
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Book] Advances in Bioinspired and Biomedical Materials Volume 2 (Chapter 11: A novel cell fusion method for direct cytoplasmic transfer using a microfluidic device)2017

    • Author(s)
      Ken-Ichi Wada, Kazuo Hosokawa, Yoshihiro Ito, Mizuo Maeda
    • Total Pages
      9
    • Publisher
      American Chemical Society
    • ISBN
      9780841232228
    • Related Report
      2017 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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