| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Outline of Final Research Achievements |
Single mitochondrion transfer is a possible approach for homoplasmic mtDNA modification. This study aimed to develop a novel method for single mitochondrion transfer and following mtDNA cloning to achieve homoplasmic mtDNA modification. In this study, I succeeded in performing single mitochondrion transfer between live single cells by achieving cell fusion through a 10 μm-length microtunnel using a microfluidic device, and in harvest of mitochondria transferred mtDNA-less cell from the microfluidic device. This provides an important basis for development of homoplasmic mtDNA modification method.
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