• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of Hydrogel Microcapsule for Single Cell Genomics

Research Project

Project/Area Number 16K07224
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field System genome science
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Aoki Hiroyoshi  国立研究開発法人理化学研究所, 光量子工学研究センター, 研究員 (50518636)

Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2017: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords1細胞ゲノム解析 / ハイドロゲル・マイクロカプセル / 難培養微生物 / アガロースゲル・マイクロカプセル / 全ゲノム増幅 / 一菌体ゲノム解析 / ハイドロゲルマイクロカプセル / 増幅バイアス / 全ゲノム酵素増幅 / シングルセル・ゲノミクス / マイクロリアクタ
Outline of Final Research Achievements

Single cell genomics (SCG) analyse genetic information of single cell from trace amount of sample. The SCG is expected to innovate biology, microbiology, and medicine. However, infinitesimal genomic DNA from single cell caused uneven enzymatic DNA amplification for DNA sequencing, which prevented to analyse all genes on the genome. To improve the uniformity of the DNA amplification, agarose gel microcapsule (AGM) is developed. AGM consists of agarose gel shell and alginate sol core with easily preparing using commercially available inexpensive reagents, plasticware, and laboratory equipment. Pico-litre-scale DNA amplification in the AGM core drastically improved the DNA amplification uniformities over 90% compared to conventional method (30%). Thus, AGM will accelerate the SCG especially for analyses of environmental microbiome, early diagnosis of cancer metastasis, and quality control of regenerative medicine.

Academic Significance and Societal Importance of the Research Achievements

1細胞染色体DNAの酵素増幅では,マイクロ流路やエマルジョン(オイル中に懸濁した微小液滴)による均一化は報告されていたが,特殊な機器が必要であり,壊れやすいエマルジョンの単離が困難,などの課題があった.本法は多くの研究者がより簡便に1細胞ゲノム解析を行えるよう,入手しやすい試薬と実験器具で作製でき,安定に単離できるAGMを開発した.学術的には半透過性のAGM内の酵素増幅による,増幅量の増加と均一性向上を示し,社会的には1細胞染色体DNAの酵素増幅の均一性の向上により,高品質の遺伝子情報を提供する手段を提供し,生物,微生物,および医学の発展に寄与した.

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (7 results)

All 2019 2017

All Presentation (5 results) (of which Int'l Joint Research: 1 results) Patent(Industrial Property Rights) (2 results)

  • [Presentation] Agarose Gel Microcapsule: Picoliter-scale Reaction Chamber for Cultivation and Next Generation DNA Sequencing2019

    • Author(s)
      Hiroyoshi Aoki and Yutaka Yamagata
    • Organizer
      7th RAP Symposium
    • Related Report
      2019 Annual Research Report
  • [Presentation] Agarose Gel Microcapsule: platform for single cell genomics and cultivation2019

    • Author(s)
      Hiroyoshi Aoki and Yutaka Yamagata
    • Organizer
      Biology-of-Symbiosis and iSYM Workshop
    • Related Report
      2019 Annual Research Report
  • [Presentation] Agarose Gel Microcapsule: Pico-liter-scale DNA Amplification Microvessel for Single Cell Genomics2017

    • Author(s)
      Hiroyoshi Aoki and Yutaka Yamagata
    • Organizer
      2017 Multi-omics for Microbiomes -- EMSL Integration Conference
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Development of Agarose Gel Microcapsule for Single Cell Genomics and Microbiome Chip2017

    • Author(s)
      青木弘良,山形豊
    • Organizer
      第5回 光量子工学研究領域シンポジウム
    • Related Report
      2017 Research-status Report
  • [Presentation] Agarose Gel Microcapsule: Pico-liter-scale DNA Amplification Microvessel for Single Cell Genomics2017

    • Author(s)
      Hiroyoshi Aoki, Yutaka Yamagata
    • Organizer
      “Biology of Symbiosis” Workshop
    • Related Report
      2017 Research-status Report
  • [Patent(Industrial Property Rights)] ハイドロゲル・マイクロカプセルの製造方法、カプセルを製造するためのキット、およびその利用2019

    • Inventor(s)
      青木 弘良,山形 豊,雪 真弘,大熊 盛也
    • Industrial Property Rights Holder
      青木 弘良,山形 豊,雪 真弘,大熊 盛也
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2019-044925
    • Filing Date
      2019
    • Related Report
      2019 Annual Research Report
  • [Patent(Industrial Property Rights)] ハイドロゲル・マイクロカプセルの製造方法、カプセルを製造するためのキット、およびその利用2019

    • Inventor(s)
      青木弘良,山形豊
    • Industrial Property Rights Holder
      理化学研究所
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2019-044925
    • Filing Date
      2019
    • Related Report
      2018 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2021-02-19  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi