Integrated global analyses of Arabidopsis deadenylase
| Project/Area Number |
16K07241
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Hokkaido University |
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Principal Investigator |
Chiba Yukako 北海道大学, 理学研究院, 准教授 (70509546)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ポリA分解酵素 / シロイヌナズナ / 統合的網羅解析 / ポリA鎖 / mRNA分解 / 翻訳制御 / ｍRNA分解 / 植物 / 発現制御 / バイオマス / ポリＡ鎖 |
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| Outline of Final Research Achievements |
To understand the function and physiological role of poly (A)-degrading enzyme in Arabidopsis thaliana, isolation of its target gene is essential. In this study, we aimed to identify the target gene and to clarify which of the mRNA amount and the translation efficiency was affected by the poly(A) length using global analyses of poly(A)-tail length, of translation efficiency, and of RNA accumulation using the mutant strain of poly (A)-degrading enzyme. We have established experimental systems and obtained reliable data on comprehensive analysis of translation efficiency and RNA accumulation. Although comprehensive analysis of poly(A)-tail length is at the preliminary stage, we will be able to understand the physiological significance of plant poly(A)-tail length control by gathering all data.
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| Academic Significance and Societal Importance of the Research Achievements |
ポリA鎖長の網羅解析，翻訳効率の網羅解析およびRNA蓄積量の網羅解析の統合は，現在，困難を極めているポリＡ分解酵素の標的遺伝子の同定を容易にし，同時にポリＡ鎖長を介した転写後制御に関する知見を飛躍的に増加させる。本研究は植物におけるその最初の挑戦であった。ポリＡ分解酵素は複数種類見つかることが多く，各酵素の機能がどのようにコーディネートされているのか，あるいは，その機能の生理学上の重要性は何であるのかは，これから解明されるべき重要な課題である。シロイヌナズナの他のポリＡ分解酵素においても，それぞれの変異株で同様の解析をおこなうことで，各酵素の役割分担を明らかにすることができる。
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Report
(4 results)
Research Products
(33 results)
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[Journal Article] Involvement of the membrane-localized ubiquitin ligase ATL8 in sugar starvation response in Arabidopsis2019
Author(s)
Luo, Y., Aoyama, S., Fukao, Y., Chiba, Y., Sato, T., and *Yamaguchi, J.
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Journal Title
Plant Biotech.
Volume: 印刷中
NAID
Related Report
Peer Reviewed
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[Journal Article] The Minimum Open Reading Frame, AUG-Stop, Induces Boron-Dependent Ribosome Stalling and mRNA Degradation.2016
Author(s)
Tanaka M., Sotta N., Yamazumi Y., Yamashita Y., Miwa K., Murota K., Chiba Y., Hirai Y. M., Akiyama T., Onouchi H., Naito S., and Fujiwara T.
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Journal Title
The Plant Cell
Volume: 28
Pages: 2830-2849
Related Report
Peer Reviewed
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[Presentation] Physiological effects of Arabidopsis deadenylases, AtCCR4a/b, components of the AtCCR4-NOT complex2019
Author(s)
Suzuki Y., Nagumo A., Arae T., Hirai M.Y., McClung C.R., Green P. J.,4, Yamaguchi J., Chiba Y.
Organizer
Post-transcriptional gene regulation in plants
Related Report
Int'l Joint Research / Invited
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[Presentation] Coordinated regulations of mRNA synthesis and degradation during cold acclimation in Arabidopsis.2017
Author(s)
Toshihiro Arae, Shiori Isai, Akira Sakai, Katsuhiko Mineta, Masami Y. Hirai, Yuya Suzuki, Shigehiko Kanaya, Junji Yamaguchi, Satoshi Naito, and Yukako Chiba
Organizer
Environmental Stress Adaptation and Memory in Plants.
Place of Presentation
RIKEN (Kanagawa, Yokohama)
Year and Date
2017-02-27
Related Report
Int'l Joint Research / Invited
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