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Elucidation of molecular nature and function of translational machineries during the course of oogenesis and development

Research Project

Project/Area Number 16K07242
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Molecular biology
Research InstitutionHokkaido University

Principal Investigator

Kotani Tomoya  北海道大学, 理学研究院, 准教授 (70419852)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords翻訳 / mRNA局在 / RNA顆粒 / 分子複合体 / 卵形成 / 初期発生 / 脊椎動物 / 高次構造 / RNA分子 / 卵母細胞
Outline of Final Research Achievements

To promote biological processes, gene products must function at appropriate timings and sites. This study aimed to elucidate the mechanisms by which gene products are produced at the appropriate timings and sites at the translational level. Our results demonstrated that mechanisms of translational regulation in oogenesis are largely coincided with those in embryogenesis. However, our results also showed that there is several differences in the mechanisms of translational regulation. These findings provide novel insights into the mechanisms of regulation of oogenesis and development.

Academic Significance and Societal Importance of the Research Achievements

卵子の形成と受精後の個体形成は、ほぼすべての生物にとって極めて重要な現象である。その進行には、卵子に蓄えられたmRNAが決まった時期に正確な場所で翻訳される必要がある。にも関わらず、その仕組みはよく分かっていない。本研究の成果は、卵子形成と個体形成に欠かせない翻訳を制御する仕組みの一端を解き明かしたものであり、すべての生物がどのように誕生し個体を形成するのか、その仕組みの解明に迫るものである。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (15 results)

All 2019 2018 2017 2016

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (11 results) (of which Int'l Joint Research: 1 results) Book (1 results)

  • [Journal Article] Pumilio1 phosphorylation precedes translational activation of its target mRNA in zebrafish oocytes2018

    • Author(s)
      Saitoh, A., Takada, Y., Horie, M., Kotani, T.
    • Journal Title

      Zygote

      Volume: 26 Issue: 5 Pages: 372-380

    • DOI

      10.1017/s0967199418000369

    • NAID

      120007171674

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High-sensitivity and high-resolution in situ hybridization of coding and long non-coding RNAs in vertebrate ovaries and testes2018

    • Author(s)
      Takei N, Nakamura T, Kawamura S, Takada Y, Satoh Y, Kimura A.P, Kotani T.
    • Journal Title

      Biological Procedures Online

      Volume: 20 Issue: 1

    • DOI

      10.1186/s12575-018-0071-z

    • NAID

      120006470991

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Formation of mos RNA granules in the zebrafish oocyte that differ from cyclin B1 RNA granules in distribution, density and regulation2016

    • Author(s)
      Horie, M., Kotani, T.
    • Journal Title

      European Journal of Cell Biology

      Volume: 95 Issue: 12 Pages: 563-573

    • DOI

      10.1016/j.ejcb.2016.10.001

    • NAID

      120007171691

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 哺乳類の卵形成と胚発生におけるPou5f1/Oct4の発現解析2019

    • Author(s)
      高田裕貴, 小谷友也
    • Organizer
      第63回日本動物学会北海道支部大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ゼブラフィッシュとマウス卵形成における翻訳調節機構2018

    • Author(s)
      小谷友也
    • Organizer
      第89回日本動物学会札幌大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] マウス卵形成および胚発生におけるPou5f1/Oct4発現解析2018

    • Author(s)
      高田裕貴, 小谷友也
    • Organizer
      第89回日本動物学会札幌大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Disassembly of Emi2 RNA granules in meiosis II is coupled with translational activation of mRNA in mouse oocytes2017

    • Author(s)
      Takei, N., Balboula, AZ., Takahashi, M., Kotani, T.
    • Organizer
      18th International Congress of Developmental Biology
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] マウス卵母細胞におけるRNA顆粒形成を介したEmi2 mRNAの翻訳制御機構の解明2017

    • Author(s)
      武井夏海, AZ Balboula, 高橋昌志, 小谷友也
    • Organizer
      第88回日本動物学会富山大会
    • Related Report
      2017 Research-status Report
  • [Presentation] マウス卵母細胞におけるEmi2 mRNAの時期特異的な翻訳制御機構2017

    • Author(s)
      武井夏海, AZ Balboula, 高橋昌志, 小谷友也
    • Organizer
      RNAフロンティアミーティング2017
    • Related Report
      2017 Research-status Report
  • [Presentation] 卵成熟における翻訳制御に重要なRNA顆粒形成の解析2016

    • Author(s)
      川村翔平, 齋藤篤, 小谷友也
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県、横浜市)
    • Year and Date
      2016-12-02
    • Related Report
      2016 Research-status Report
  • [Presentation] 卵母細胞の減数分裂進行におけるRNA顆粒形成を介した翻訳制御機構2016

    • Author(s)
      川村翔平, 齋藤篤, 小谷友也
    • Organizer
      RNAフロンティアミーティング2016
    • Place of Presentation
      北海道ニセコいこいの湯宿いろは(北海道、ニセコ町)
    • Year and Date
      2016-09-02
    • Related Report
      2016 Research-status Report
  • [Presentation] マウス卵母細胞においてMad2 mRNAの翻訳を制御するメカニズム2016

    • Author(s)
      川村翔平, 齋藤篤, 小谷友也
    • Organizer
      日本動物学会北海道支部第61回大会
    • Place of Presentation
      旭川医科大学臨床講義棟臨床第2講義室(北海道、旭川市)
    • Year and Date
      2016-08-27
    • Related Report
      2016 Research-status Report
  • [Presentation] 卵母細胞において減数分裂第二分裂で翻訳されるEmi2 mRNAの制御機構解析2016

    • Author(s)
      武井夏海, AZ balboula, 高橋昌志, 小谷友也
    • Organizer
      日本動物学会北海道支部第61回大会
    • Place of Presentation
      旭川医科大学臨床講義棟臨床第2講義室(北海道、旭川市)
    • Year and Date
      2016-08-27
    • Related Report
      2016 Research-status Report
  • [Presentation] ゼブラフィッシュpou2 mRNAの卵成熟から初期発生における翻訳制御機構の解析2016

    • Author(s)
      前畑香織, 小谷友也
    • Organizer
      日本動物学会北海道支部第61回大会
    • Place of Presentation
      旭川医科大学臨床講義棟臨床第2講義室(北海道、旭川市)
    • Year and Date
      2016-08-27
    • Related Report
      2016 Research-status Report
  • [Book] Regulation of translationally repressed mRNAs in zebrafish and mouse oocytes. In: Klock, M., ed. Oocytes: Maternal information and functions. Results and Problems in Cell Differentiation2017

    • Author(s)
      Kotani T, Maehata K, Takei N.
    • Total Pages
      27
    • Publisher
      Springer Nature
    • Related Report
      2017 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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