Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Outline of Final Research Achievements |
Cdc7 protein functions as a protein kinase which plays an important role in DNA replication. Small-molecule inhibitors of Cdc7 kinase are currently expected as novel anticancer drugs. Cdc7 kinase activity is positively regulated by binding to ASK protein. The molecular mechanism by which ASK protein is stabilized/destabilized is still unknown. In this research project, I found that a deubiquitinase USP7 stabilizes ASK protein, which leads to activation of Cdc7 kinase. In addition, I also found that Cdc7 kinase inhibition induces replication fork collapses caused by dissociation of some proteins of DNA replication machinery.
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