Molecular basis of acceleration of base-pairing between sRNA and target mRNA by Hfq

• Project/Area Number: 16K07259
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Suzuka University of Medical Science
• Principal Investigator: Morita Teppei 鈴鹿医療科学大学, 薬学部, 助教 (10444366)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: small RNA / Hfq / 転写終結 / ステムループ構造 / 小分子RNA / 転写後制御 / 大腸菌

Outline of Final Research Achievements

This study clarified a role of the terminator stem-loop of small regulatory RNA (sRNA) in the production of sRNAs in E. coli. We also demonstrated the mechanism by which expression of hfq gene encoding Hfq is strictly auto-regulated by Hfq. A biological significance of the Hfq autoregulation is to prevent overexpression of Hfq, which is toxic to cell growth.

Academic Significance and Societal Importance of the Research Achievements

本研究成果は、sRNA生合成において、sRNA機能を生み出す転写終結の意義を明らかにした。近年、sRNAは細菌感染症やバイオフィルムといった人間社会に関わる細菌作用に重要な役割を持つことが次々に報告されている。そのため、本研究成果は、sRNA生合成の解明という学術的意義に加え、抗菌薬の開発のための基盤知見になる。

Research Products

• [Int'l Joint Research] National Institutes of Health(米国)
• [Journal Article] Mechanism and physiological significance of autoregulation of the Escherichia coli hfq gene (2018)
  • Author(s): Morita Teppei、Aiba Hiroji
  • Journal Title: RNA Volume: 25 Issue: 2 Pages: 264-276
  • DOI: 10.1261/rna.068106.118
  • Peer Reviewed / Open Access
• [Journal Article] Functional structure and biogenesis of bacterial small RNA (2017)
  • Author(s): 森田 鉄兵
  • Journal Title: 生化学 Volume: 89 Issue: 4 Pages: 551-554
  • DOI: 10.14952/SEIKAGAKU.2017.890551
  • NAID: 40021312496
  • ISSN: 0037-1017
  • Year and Date: 2017-08-25
  • Open Access
• [Journal Article] Role of the terminator hairpin in the biogenesis of functional Hfq-binding sRNAs (2017)
  • Author(s): Morita Teppei、Nishino Ryo、Aiba Hiroji
  • Journal Title: RNA Volume: 23 Issue: 9 Pages: 1419-1431
  • DOI: 10.1261/rna.060756.117
  • Peer Reviewed / Open Access
• [Journal Article] Inhibition of Phagocytic Killing of Escherichia coli in Drosophila Hemocytes by RNA Chaperone Hfq. (2016)
  • Author(s): Shiratsuchi, A., Nitta, M., Kuroda, A., Komiyama, C., Gawasawa, M., Shimamoto, N., Tuan, T. Q., Morita, T., Aiba, H.,and Nakanishi, Y.
  • Journal Title: J. Immunol. Volume: 197 Issue: 4 Pages: 1298-1307
  • DOI: 10.4049/jimmunol.1501953
  • Peer Reviewed / Open Access
• [Presentation] RNAシャペロンHfqの自己発現制御機構と生理学的意義 (2018)
  • Author(s): 森田鉄兵、饗場弘二
  • Organizer: 第20回RNA学会年会（大阪）
• [Presentation] RNAシャペロンHfqの自己発現制御機構 (2018)
  • Author(s): 森田鉄兵、饗場弘二
  • Organizer: 第15回21世紀大腸菌研究会（山形）
• [Presentation] RNAシャペロンHfqの自己発現制御機構と生物学的意義 (2018)
  • Author(s): 森田 鉄兵、饗場 弘二
  • Organizer: 第12回長野ミーティング（長野）
• [Presentation] Mechanism and physiological significance of autoregulation of the Escherichia coli hfq gene (2018)
  • Author(s): 森田 鉄兵、饗場 弘二
  • Organizer: 5th Meeting of Regulating with RNA in Bacteria & Archaea (Seville, Spain)
  • Int'l Joint Research
• [Presentation] Hfq結合性sRNAの生合成におけるターミネーターヘアピンの役割 (2017)
  • Author(s): 森田 鉄兵、西野 良、饗場 弘二
  • Organizer: 第14回21世紀大腸菌研究会（熱海）
• [Presentation] Hfq結合性sRNAの生合成におけるターミネーターヘアピンの役割 (2017)
  • Author(s): 森田 鉄兵、西野 良、饗場 弘二
  • Organizer: 第19回RNA学会年会（富山）
• [Presentation] Role of terminator hairpin in biogenesis of functional Hfq-binding sRNAs (2017)
  • Author(s): 森田 鉄兵、西野 良、饗場 弘二
  • Organizer: 15th Asian Conference on Transcription (Penang, Malaysia)
  • Int'l Joint Research
• [Presentation] Hfq結合型small RNAの機能構造の解析 (2016)
  • Author(s): 森田 鉄兵、饗場 弘二
  • Organizer: 日本進化学会第18回大会
  • Place of Presentation: 東京工業大学 大岡山キャンパス(東京)
  • Year and Date: 2016-08-25
• [Presentation] Rho-independent terminator of Hfq-binding sRNAs genes is suitably organized to generate functional sRNAs under stress condition in E. coli. (2016)
  • Author(s): Teppei Morita, Rho Nishino and Hiroji Aiba
  • Organizer: The 21st Annual Meeting of the RNA Society, The RNA Society of Japan 18th Annual Meeting
  • Place of Presentation: Kyoto International Conference Center( Kyoto,Japan)
  • Year and Date: 2016-06-28
  • Int'l Joint Research
• [Presentation] 大腸菌Hfq結合型sRNA遺伝子のRho非依存的ターミネーターは機能的なsRNAを産生するのに適した構成である (2016)
  • Author(s): 森田 鉄兵、西野 良、饗場 弘二
  • Organizer: 第13回21世紀大腸菌研究会
  • Place of Presentation: グリーンピア南阿蘇(熊本県阿蘇郡)
  • Year and Date: 2016-06-02