Structural analyses of reaction states of ion-pumping rhodopsins
| Project/Area Number |
16K07267
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
井原 邦夫 名古屋大学, 遺伝子実験施設, 准教授 (90223297)
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| Project Period (FY) |
2016-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | イオン能動輸送 / 光駆動塩素イオンポンプ / 光駆動プロトンポンプ / レチナールタンパク質 / レチナール異性体 / X線結晶構造解析 / ハロロドプシン / 光駆動陰イオンポンプ / レチナール光異性化 / アーキロドプシン / プロトン輸送性ロドプシン / 光駆動イオンポンプ / バクテリオロドプシン / 陰イオン輸送性ロドプシン / レチナール異性化 / 光誘起吸収変化 / 低温原子間力顕微鏡 / レチナール・タンパク質 / 陰イオン輸送 / プロトン輸送 |
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| Outline of Final Research Achievements |
The anion pumping cycle of halorhodopsin is initiated when the all-trans/15-anti isomer of retinal is photo-isomerized into the 13-cis/15-anti configuration. A previous study suggested that a reaction state with 13-cis/15-syn retinal occurred during the anion release process, i.e., after the N state with the 13-cis/15-anti retinal and before the O state with all-trans/15-anti retinal. In this study, we found that the 13-cis isomer (HR'), in which the absorption spectrum was blue-shifted by 8 nm as compared with that of the trans isomer (HR), accumulated significantly when pHR-rich claret membranes in 4 M NaBr was illuminated with continuous light. It was suggested that the branching of the trans photocycle into the 13-cis isomer (HR’) occurs during the decay of an O-like state (O’) with 13-cis/15-syn retinal. At a high bromide ion concentration, the anion pumping cycle is described by the scheme: HR -(hν)-> K -> L1 -> N -> N’ -> O’ -> HR’-> HR.
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| Academic Significance and Societal Importance of the Research Achievements |
ハロロドプシンの陰イオン輸送サイクル中に3段階のレチナール異性化反応（13-trans/15-anti―→13-cis/15-anti―→13-cis/15-syn―→13-trans/15-anti）が起こることを明らかにした。タンパク質部分も反応サイクルの間に3種類の構造変化を起こすことが示されており、これらの結果を基に、レチナールの異性化→タンパク質の構造変化→イオン移動→レチナールの異性化、…、という具合に反応が進行することで、イオンの一方向の輸送が保証される、という作業仮説を提唱した。これは、ロドプシン群タンパク質の光反応機構に関する従来の基本的な考えを大きく変えることになる。
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Report
(5 results)
Research Products
(38 results)
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[Journal Article] The mechanosensitive channel YbdG from Escherichia coli has a role in adaptation to osmotic up-shock.2019
Author(s)
Shun Amemiya, Hayato Toyoda, Mami Kimura, Hiromi Saito, Hiroshi Kobayashi, Kunio Ihara 3 , Kiyoto Kamagata 4 , Ryuji Kawabata 5 , Setsu Kato, Yutaka Nakashimada, Tadaomi Furuta, Shin Hamamoto, Nobuyuki Uozumi
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Journal Title
Journal of Biological Chemistry
Volume: 294 (33)
Issue: 33
Pages: 12281-12292
DOI
Related Report
Peer Reviewed
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[Journal Article] Salinarchaeum chitinilyticum sp. nov., a chitin-degrading haloarchaeon isolated from commercial salt2017
Author(s)
Minegishi H, Enomoto S, Echigo A, Shimane Y, Kondo Y, Inoma A, Kamekura M, Takai K, Itoh T, Ohkuma M, Ihara K, Takahashi-Ando N, Fukushima Y, Ishii S, Yoshida Y, Usami R
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Journal Title
Int J Syst Evol Microbiol.
Volume: 67
Issue: 7
Pages: 2274-2278
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Shifting transcriptional machinery is required for long-term memory maintenance and modification in Drosophila mushroom bodies.2016
Author(s)
Hirano Y, Ihara K, Masuda T, Yamamoto T, Iwata I, Takahashi A, Awata H, Nakamura N, Takakura M, Suzuki Y, Horiuchi J, Okuno H, Saitoe M.
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Journal Title
Nat Commun.
Volume: 7
Issue: 1
Pages: 13471-13471
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Photochemical characterization of actinorhodopsin and its functional existence in the natural host2016
Author(s)
Nakamura, S., Kikukawa, T., Tamogami, J., Kamiya, M., Aizawa, T., Hahn, M., Ihara, K., Kamo, N., Demura, M.
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Journal Title
BBA-BIOENERGETICS
Volume: 1857
Issue: 12
Pages: 1900-1908
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Haloparvum alkalitolerans sp. nov., alkali-tolerant haloarchaeon isolated from commercial salt.2016
Author(s)
Kondo Y, Minegishi H, Echigo A, Shimane Y, Kamekura M, Itoh T, Ohkuma M, Tanaka A, Takahashi-Ando N, Fukushima Y, Yoshida Y, Ihara K, Usami R
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Journal Title
Int J Syst Evol Microbiol
Volume: 66
Issue: 12
Pages: 5314-5319
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] FliH and FliI ensure efficient energy coupling of flagellar type III protein export in Salmonella.2016
Author(s)
Minamino T, Kinoshita M, Inoue Y, Morimoto YV, Ihara K, Koya S, Hara N, Nishioka N, Kojima S, Homma M, Namba K
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Journal Title
Microbiologyopen
Volume: -
Issue: 3
Pages: 340-340
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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