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Molecular design of quadruplex-forming nculeic acids that inhibit the pathological conformational conversion of PrP and elucidation of its inhibitory mechanism

Research Project

Project/Area Number 16K07269
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Structural biochemistry
Research InstitutionKyoto University

Principal Investigator

Mashima Tsukasa  京都大学, エネルギー理工学研究所, 助教 (20707426)

Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsNMR / アプタマー / 四重鎖核酸 / プリオン病 / 立体構造
Outline of Final Research Achievements

Prion diseases are neurodegenerative disorders caused by the conversion of cellular prion protein to pathological isoform. Previously, we obtained an RNA aptamer, R12, that possesses anti-prion activity. We also revealed that R12 folds into a unique G-quadruplex structure and two R12 molecules form a homodimer. Here, we developed new prion aptamers, R24 and R12-A-R12, based on the structure of R12 homodimer. The assay with prion-infected cells showed that both aptamers exhibited much higher anti-prion activity than R12. Our NMR studies showed that the structure of a single R12-A-R12 molecule resembled that of the R12 homodimer. R24 is supposed to unimolecularly form a similar structure of R12 homodimer because the sequence of R24 is almost the same as that of R12-A-R12. The quadruplex structure of either R24 or R12-A-R12 formed by one molecule could be stable when they are administered to a prion-infected cell culture. This may be the reason they possess high anti-prion activity.

Academic Significance and Societal Importance of the Research Achievements

プリオン病は重篤な神経変性疾患であるが、根治できる治療法がなく、効果的な治療薬の開発が望まれている。今回我々が開発したアプタマーは、ある種の抗体などを除き、これまでの抗プリオン物質の中で最も高い効率でプリオンタンパク質の異常化を抑制する。よって治療薬への応用が期待されるため、社会的意義がある。
核酸、特に四重鎖構造を有する核酸は一塩基を置換・付加しただけで大きくそのトポロジーが変わることがあり、その原理は不明である。本研究では新規アプタマーを設計し、その立体構造が意図した通りであるかを確認し、さらに活性を大きく向上させることに成功したため、学術的にも意義がある。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (38 results)

All 2020 2019 2018 2017 2016 Other

All Int'l Joint Research (1 results) Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 7 results,  Open Access: 7 results) Presentation (30 results) (of which Int'l Joint Research: 10 results)

  • [Int'l Joint Research] Gyeongsang National University(韓国)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Development and structural determination of an anti-PrPC aptamer that blocks pathological conformational conversion of prion protein2020

    • Author(s)
      Mashima Tsukasa、Lee Joon-Hwa、Kamatari Yuji O.、Hayashi Tomohiko、Nagata Takashi、Nishikawa Fumiko、Nishikawa Satoshi、Kinoshita Masahiro、Kuwata Kazuo、Katahira Masato
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 4934-4934

    • DOI

      10.1038/s41598-020-61966-4

    • NAID

      120006813315

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] RNA sequence and length contribute to RNA-induced conformational change of TLS/FUS2020

    • Author(s)
      Hamad Nesreen、Mashima Tsukasa、Yamaoki Yudai、Kondo Keiko、Yoneda Ryoma、Oyoshi Takanori、Kurokawa Riki、Nagata Takashi、Katahira Masato
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 2629-2629

    • DOI

      10.1038/s41598-020-59496-0

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The anti‐prion RNA aptamer R12 disrupts the Alzheimer's disease‐related complex between prion and amyloid β2019

    • Author(s)
      Iida Mamiko、Mashima Tsukasa、Yamaoki Yudai、So Masatomo、Nagata Takashi、Katahira Masato
    • Journal Title

      The FEBS Journal

      Volume: 印刷中 Issue: 12 Pages: 2355-2365

    • DOI

      10.1111/febs.14819

    • Related Report
      2019 Annual Research Report 2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Significance of a histone-like protein with its native structure for the diagnosis of asymptomatic tuberculosis2018

    • Author(s)
      Ohara Yukiko、Ozeki Yuriko、Tateishi Yoshitaka、Mashima Tsukasa、Arisaka Fumio、Tsunaka Yasuo、Fujiwara Yoshie、Nishiyama Akihito、Yoshida Yutaka、Kitadokoro Kengo、Kobayashi Haruka、Kaneko Yukihiro、Nakagawa Ichiro、Maekura Ryoji、Yamamoto Saburo、Katahira Masato、Matsumoto Sohkichi
    • Journal Title

      PLOS ONE

      Volume: 13 Issue: 10 Pages: 0204160-0204160

    • DOI

      10.1371/journal.pone.0204160

    • NAID

      120006534592

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Plastic roles of phenylalanine and tyrosine residues of TLS/FUS in complex formation with the G-quadruplexes of telomeric DNA and TERRA2018

    • Author(s)
      Kondo Keiko、Mashima Tsukasa、Oyoshi Takanori、Yagi Ryota、Kurokawa Riki、Kobayashi Naohiro、Nagata Takashi、Katahira Masato
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 2864-2864

    • DOI

      10.1038/s41598-018-21142-1

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] DNA G-wire formation using an artificial peptide is controlled by protease activity2017

    • Author(s)
      Usui, K., Okada, A., Sakashita, S., Shimooka, M., Tsuruoka, T., Nakano, S., Miyoshi, D., Mashima, T., Katahira, M., Hamada, Y.
    • Journal Title

      Molecules

      Volume: 22 Issue: 11 Pages: 1991-1991

    • DOI

      10.3390/molecules22111991

    • NAID

      120006501324

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of an RNA aptamer that acquires binding capacity against HIV-1 Tat protein via G-quadruplex formation in response to potassium ions2017

    • Author(s)
      Yamaoki, Y., Nagata, T., Mashima, T., Katahira, M.
    • Journal Title

      Chemical communications

      Volume: 53 Issue: 52 Pages: 7056-7059

    • DOI

      10.1039/c7cc03312e

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Development of anti-prion RNA aptamers and destruction of the Alzheimer's disease-related complex2019

    • Author(s)
      Mashima, T., Iida, M., Lee, J., Yamaoki, Y., So, M., Kamatari, Y. O., Hayashi, T., Kinoshita, M., Kuwata, K., Nagata, T. and Katahira, M.
    • Organizer
      The 46th International Symposium on Nucleic Acids Chemistry
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Disruption of the Alzheimer’s disease-related complex between prion and amyloid β by an anti-prion aptamer2019

    • Author(s)
      Mashima, T., Iida, M., Yamaoki, Y., So, M., Nagata, T. and Katahira, M.
    • Organizer
      Commemorative International Symposium of Nucleic Acid Chemistry
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 非常に高い活性を有する抗プリオンRNAアプタマーの構造機能相関2019

    • Author(s)
      真嶋 司、Lee, Joon-Hwa、鎌足 雄司、林 智彦、木下 正弘、桑田 一夫、永田 崇、片平 正人
    • Organizer
      第58回NMR討論会
    • Related Report
      2019 Annual Research Report
  • [Presentation] プリオンタンパク質の構造変換を抑制するRNAアプタマーの開発と構造決定2019

    • Author(s)
      真嶋 司、Lee, Joon-Hwa、鎌足 雄司、林 智彦、永田 崇、西川 富美子、西川 諭、木下 正弘、桑田 一夫、片平 正人
    • Organizer
      第21回日本RNA学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Development of RNA Aptamer That Has High Anti-prion Activity and Its Structural Basis2018

    • Author(s)
      Mashima, T., Lee, J., Kamatari, Y. O., Hayashi, T., Nishikawa, F., Nagata, T., Nishikawa, S., Kinoshita, M., Kuwata, K. and Katahira, M.
    • Organizer
      The 45th International Symposium on Nucleic Acids Chemistry
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 抗プリオン活性を有する四重鎖RNAの構造決定と高い活性の発現機構の解明2018

    • Author(s)
      真嶋司、Lee Joon-Hwa、鎌足雄司、林智彦、西川富美子、永田崇、西川諭、木下正弘、桑田一夫、片平正人
    • Organizer
      第57回NMR討論会
    • Related Report
      2018 Research-status Report
  • [Presentation] An RNA aptamer disrupts the interaction of prion protein with Amyloid β2018

    • Author(s)
      Iida, M., Mashima, T., Yamaoki, Y., So, M., Nagata, T. and Katahira, M.
    • Organizer
      第56回日本生物物理学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] The effect of different target nucleic acids on the structural change of TLS2017

    • Author(s)
      Hamad, N., Mashima, T., Watanabe, H., Uchihashi, T., Kurokawa, R., Nagata, T. Katahira, M.
    • Organizer
      2017年度生命科学系学会合同年次大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Interaction between non-coding RNA and TLS/FUS protein, and structural change2017

    • Author(s)
      Mashima, T., Nagata, T., Hamad, N., Ozawa, S., Yamaoki, Y., Kondo, K., Watanabe, H., Yoneda, R., Uchihashi, T., Kurokawa, R. Katahira, M.
    • Organizer
      The 44th International Symposium on Nucleic Acids Chemistry
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 非コードRNAとTLS/FUSタンパク質の相互作用様式と結合に伴うTLS/FUSの大きな構造変化2017

    • Author(s)
      真嶋司,ハマッド ネスリン,小澤駿介,渡辺大輝,内橋貴之,米田竜馬,黒川理樹,永田崇,片平正人
    • Organizer
      第56回NMR討論会
    • Related Report
      2017 Research-status Report
  • [Presentation] テロメア長短縮をもたらすTLS/FUS蛋白質とテロメアDNAおよびTERRAのグアニン四重鎖との複合体に関するNMR解析2017

    • Author(s)
      近藤敬子, 真嶋司, 大吉崇文, 黒川理樹, 小林直宏, 永田崇, 片平正人
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Research-status Report
  • [Presentation] プリオンアプタマーはアルツハイマー病に関与するプリオン蛋白質と Aβ オリゴマーの複合体の形成を阻害する2017

    • Author(s)
      飯田真美子, 真嶋司, 山置佑大, 永田崇, 片平正人
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Research-status Report
  • [Presentation] TLS/FUS の非コード RNA の認識機構の構造学的研究2017

    • Author(s)
      真嶋司,小澤 駿介, N. Hamad, 米田 竜馬, 黒川理樹,永田崇,片平正人
    • Organizer
      第19回日本RNA学会年会
    • Related Report
      2017 Research-status Report
  • [Presentation] Conformational change detection of translocated in liposarcoma, TLS, upon binding to various RNAs2017

    • Author(s)
      Hamad, N., Mashima, T., Yamaoki, Y., Kondo, K., Kurokawa, R., Nagata, T. Katahira, M.
    • Organizer
      第19回日本RNA学会年会
    • Related Report
      2017 Research-status Report
  • [Presentation] TLS/FUS のRNA認識機構と結合に伴うコンフォメーション変化の研究2017

    • Author(s)
      真嶋司,小澤 駿介, N. Hamad, 米田 竜馬, 黒川理樹,永田崇,片平正人
    • Organizer
      第17回日本蛋白質科学会年会
    • Related Report
      2017 Research-status Report
  • [Presentation] Invention of RNA aptamer and ribozyme whose activities switch on in response to potassium ion via G-quadruplex formation2017

    • Author(s)
      Yamaoki, Y., Mashima, T., Nagata, T. Katahira, M.
    • Organizer
      6th International Meeting on Quadruplex Nucleic Acids
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 三者複合体形成によってテロメア短縮をもたらすTLS/FUSのテロメアDNAおよびTERRA認識機構に関するNMR解析2016

    • Author(s)
      近藤敬子, 真嶋司, 大吉崇文, 永田崇, 片平正人
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜、神奈川県
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report
  • [Presentation] 非コードRNAと結合してcyclin D1転写を抑制するTLS/FUSのRNA認識機構の構造学的基盤2016

    • Author(s)
      真嶋司, 小澤駿介, 近藤敬子, Nesreen Hamad, 米田竜馬, 黒川理樹, 永田崇, 片平正人
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜、神奈川県
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report
  • [Presentation] プリオンタンパク質とプリオンタンパク質を標的とする RNA 分子の Aβ線維化への影響2016

    • Author(s)
      M. Iida, T. Mashima, Y. Yamaoki, T. Nagata
    • Organizer
      生物物理学会第54回年会
    • Place of Presentation
      つくば国際会議場、茨城県
    • Year and Date
      2016-11-25
    • Related Report
      2016 Research-status Report
  • [Presentation] Cyclin D1遺伝子転写抑制やテロメア長制御に関わる TLS/FUS タンパク質と非コード核酸間の相互作用のNMR法による解析2016

    • Author(s)
      近藤敬子, 真嶋司, 大吉崇文, 米田竜馬, 黒川理樹, 永田崇, 片平正人
    • Organizer
      第55回NMR討論会
    • Place of Presentation
      広島国際会議場、広島県
    • Year and Date
      2016-11-16
    • Related Report
      2016 Research-status Report
  • [Presentation] C:G塩基対を選択的に認識する人工塩基を含む三重鎖DNAの構造解析2016

    • Author(s)
      真嶋司、岡村秀紀、王磊、谷口陽祐、佐々木茂貴、片平正人
    • Organizer
      第55回NMR討論会
    • Place of Presentation
      広島国際会議場、広島県
    • Year and Date
      2016-11-16
    • Related Report
      2016 Research-status Report
  • [Presentation] リグノセルロースバイオリファイナリーのためのリグニン・ペプチド間相互作用解析2016

    • Author(s)
      山口亜佐子,末富高志,大城理志,徳永有希,西村裕司,永田崇,真嶋司,片平正人,礒﨑勝弘,高谷光,中村正治,渡辺隆司
    • Organizer
      第61回リグニン討論会
    • Place of Presentation
      京都大学、京都府
    • Year and Date
      2016-10-27
    • Related Report
      2016 Research-status Report
  • [Presentation] Non-coding RNA/DNA recognition by TLS/FUS that causes repression of cyclin D1 transcription and telomere elongation2016

    • Author(s)
      K. Kondo, T. Mashima, T. Oyoshi, R. Yoneda, R. Kurokawa, T. Nagata, M. Katahira
    • Organizer
      The 43rd International Symposium on Nucleic Acids Chemistry
    • Place of Presentation
      熊本大学、熊本県
    • Year and Date
      2016-09-27
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] カリウムイオン応答性Tat結合RNAアプタマーおよびハンマーヘッドリボザイムの創製および in-cell NMR法による細胞内核酸の観測2016

    • Author(s)
      山置佑大, 清石彩華, 真嶋司, 加納ふみ, 村田昌之, 永田崇, 片平正人
    • Organizer
      第10回バイオ関連化学シンポジウム
    • Place of Presentation
      県立音楽堂、石川県
    • Year and Date
      2016-09-07
    • Related Report
      2016 Research-status Report
  • [Presentation] NMR studies of non-coding RNA and DNA recognition by TLS/FUS which induces transcriptional repression of cyclin D1 and telomere shortening2016

    • Author(s)
      K. Kondo, T. Mashima, T. Oyoshi, R. Yoneda, R. Kurokawa, T. Nagata, M. Katahira
    • Organizer
      The XXVIIth International Conference on Magnetic Resonance in Biological Systems
    • Place of Presentation
      京都国際会館、京都府
    • Year and Date
      2016-08-21
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] K+-responsive activation of dual-quadruplex forming ribozyme system requiring no heating and cooling treatment2016

    • Author(s)
      Y. Yamaoki, T. Mashima, T. Nagata, M. Katahira
    • Organizer
      The 21st Annual Meeting of the RNA Society
    • Place of Presentation
      京都国際会館、京都府
    • Year and Date
      2016-06-28
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] NMR analysis of the recognition of non-coding RNA and DNA by TLS/FUS which causes transcriptional repression of cyclin D1 1 and telomere shorteningThe 21st Annual Meeting of the RNA Society2016

    • Author(s)
      T. Mashima. K. Kondo, T. Oyoshi, R. Yoneda, R. Kurokawa, T. Nagata, M. Katahira
    • Organizer
      The 21st Annual Meeting of the RNA Society
    • Place of Presentation
      京都国際会館、京都府
    • Year and Date
      2016-06-28
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] プリオン蛋白質とそれを標的とする RNA 分子の Aβ線維化への影響2016

    • Author(s)
      飯田真美子, 真嶋司, 山置佑大, 永田崇, 片平正人
    • Organizer
      第16回日本蛋白質科学会年会
    • Place of Presentation
      福岡国際会議場、福岡県
    • Year and Date
      2016-06-07
    • Related Report
      2016 Research-status Report
  • [Presentation] Cyclin D1転写抑制を担う TLS/FUS タンパク質と非コードRNA の相互作用の NMR 法による解析2016

    • Author(s)
      真嶋司, 小澤駿介, 近藤敬子, 永田崇, 黒川理樹, 片平正人
    • Organizer
      第16回日本蛋白質科学会年会
    • Place of Presentation
      福岡国際会議場、福岡県
    • Year and Date
      2016-06-07
    • Related Report
      2016 Research-status Report
  • [Presentation] K+-responsive activation of ribozyme system via structural transition from duplex to dual-quadruplex requiting no heating and cooling treatment2016

    • Author(s)
      Y. Yamaoki, T. Mashima, T. Nagata, M. Katahira
    • Organizer
      The XXVIIth International Conference on Magnetic Resonance in Biological Systems
    • Place of Presentation
      京都国際会館、京都府
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research

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Published: 2016-04-21   Modified: 2021-02-19  

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