| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
To understand Th1 cell differentiation mechanism from naive T cell, we study the structural analysis of interleukin (IL) -27 complexed with two receptors WSX-1 and gp130.
Preparation of these three proteins was carried out, and then IL-27 / WSX-1 complex was successfully purified. The crystallization trials were attempted using purified IL-27 / WSX-1 complex . And preparation trials of IL-27 / WSX-1 / gp130 complex were failed due to the weak binding ability of gp130 to IL-27 / WSX-1 complex. We are currently redesigning the gp130 construct to obtain crystal of IL-27 / WSX-1 / gp130 complex.
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