| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
A mouse lacking Arf1 only in neurons dies shortly after weaning with movement disorder of the hind limbs. We clarified the decrease in the number of granule cells in the cerebellum. We succeeded in creating a Fucci-expressing cell line capable of analyzing the doubling time and cell cycle in Arf1-deficient MEF cells. When tissue-specific deletion of Arf 1 and/or 6 in T and B lymphocytes were induced, abnormalities in the immune mechanism (such as abnormal maturation and abnormal antibody secretion) were found. We succeeded in generating mice for the preparation of Arf1 & 6 double-deficient MEF cells. In the SMAP1 & 2 double-deficient cells, we found that the signal loss is specifically delayed in the activity of the BMP signal (duration of the activity).
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