| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Histone transcription is rapidly repressed on arrest of DNA replication, but the molecular mechanism of this coupling has not been elucidated. I previously reported that histone gene transcription is regulated by the transcription factor Ams2 in fission yeast. Therefore, I screened for factors that interact with Ams2 and identified a Cds1, which is DNA damage checkpoint factor. The interaction between Ams2 and Cds1 was confirmed by yeast two-hybrid analysis and co-immunoprecipitation assay. In Cds1 deletion mutant cells, the amounts of histone transcription were reduced and binding of Ams2 to the histone promoter region was unstable under DNA damage reagent. In contrast, Cds1 did not bind to the histone promoter, I hypothesized that Cds1 is indirectly involved in the regulation of histone transcription. These results are new finding suggesting that DNA replication checkpoint factor is involved in the regulation of histone transcription.
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