| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
The spatial and temporal variation of intracellular signaling may play role in determining cell fates by inducing different cellular functions.In this study, the dynamics of intracellular kinases in living cells was investigated by developing the novel tools to quantitatively evaluate and control kinase activity for understanding its physiological significance. It was found that inflammatory IL-1beta stimuli elicits JNK activity only transiently because of the negative feedback inhibitory mechanism lying downstream of p38. In addition, optical control of p38 activity in living cells was achieved by developing a new tool which is composed of light-responsive domains and a kinase domain.
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