| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
tRNA is extensively modified after transcription to fulfill its functions in translation. Two-thiouridines in tRNAs are essential for recognition of split-codon boxes and stabilization of the whole structure of tRNAs. We have investigated the biosynthesis mechanism of these thiouridines.
We identified ttuD as a gene for the synthesis of s2T54 in Thermus thermophilus. TtuD receives the persulfide generated by cysteine desulfurases and enhances the formation of thiocarboxylated TtuB, the sulfur donor for the tRNA sulfurtransferase TtuA. We also demonstrated by spectroscopic, biochemical, and crystal structure analyses that TtuA and other sulfurtransferases require oxygen-labile [4Fe-4S]-type iron - sulfur clusters for its enzymatic activity. We propose a novel molecular mechanism of sulfur transfer by these enzymes.
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