Studies on the transition state of protein folding by means of correlation analysis between the folding rate and the native structure
| Project/Area Number |
16K07314
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Kuwajima Kunihiro 東京大学, 大学院理学系研究科(理学部), 名誉教授 (70091444)
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| Project Period (FY) |
2016-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | フォールディング / 速度論 / 遷移状態 / 蛋白質 / 生物物理 |
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| Outline of Final Research Achievements |
In this study, (1) a standardized protein folding database, in which the folding rate constant of all listed proteins are calculated at the standard temperature (25°C), has been constructed and opened on the internet homepage. (2) The correlations between the Φ values, which represent the degree of structural formation in the transition state of folding, and different structure-based properties of proteins were analyzed, and the relationships between the structure-based properties and the critical structure in the transition state were discussed. (3) Academic literatures concerning the relationships between the folding rate constant and various structure-based properties were investigated, and it has been found that the two-state and the non-two-state folding reactions of proteins apparently seem very different, but are based on essentially the same physical principles.
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| Academic Significance and Societal Importance of the Research Achievements |
蛋白質フォールディング機構の研究は，蛋白質の間違ったフォールディングによって引き起こされる，ヒトの様々なフォールディング病（アルツハイマー病，白内障，クロイツフェルト・ヤコブ病，パーキンソン病，II型糖尿病など多数）などの原因解明や治療法の構築の応用研究に役立つと期待される，基盤的な研究である。本研究で構築されたデータベースは，蛋白質フォールディングの計算機シミュレーションや理論研究の研究者によって，広く活用されることが期待される。本研究で実施された，Φ値やフォールディング速度と構造特性との間の相関解析は，本データベースを活用するための事例研究として用いることも出来る。
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Report
(5 results)
Research Products
(23 results)
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[Presentation] The problem of protein folding2016
Author(s)
Kuwajima, K., Manavalan, B. & Lee, J.
Organizer
The 16th KIAS Conference on Protein Structure
Place of Presentation
Seoul (Korea)
Year and Date
2016-09-22
Related Report
Int'l Joint Research / Invited
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[Presentation] The problem of protein folding2016
Author(s)
Kuwajima, K., Manavalan, B. & Lee, J.
Organizer
The 2nd Polish-Korean Conference on Protein Folding: Theoretical and Experimental Approaches
Place of Presentation
Gdansk (Poland)
Year and Date
2016-05-28
Related Report
Int'l Joint Research / Invited
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