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Spatial separation and decoding of brain cell calcium signals

Research Project

Project/Area Number 16K07316
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biophysics
Research InstitutionWaseda University (2019)
Institute of Physical and Chemical Research (2016-2018)

Principal Investigator

Bannai Hiroko  早稲田大学, 理工学術院, 教授 (40332340)

Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywordsカルシウムイメージング / カルシウムシグナル / 細胞 / アストロサイト / カルシウムチャネル / 新規カルシウムセンサー / 高感度 / 高解像度 / カルシウム / 神経細胞 / グリア細胞 / イメージング / プローブ開発 / マルチカラーイメージング / AMPA受容体 / 遺伝子コード型カルシウムセンサー / 神経 / シナプス可塑性
Outline of Final Research Achievements

Organisms use Ca2+ as a transmitter to induce diverse physiological functions. However, it remains unclear how Ca2+ encodes diverse information. We created “local Ca2+ sensors” that enable us to identify the origin of Ca2+ signals and to describe the diversity of Ca2+ signals. Using local Ca2+ sensors, we analyzed the spontaneous Ca2+ signals in the astrocyte, which is important for the regulation of the brain. We elucidated the molecular mechanisms underlying the maintain Ca2+ homeostasis in hippocampal astrocytes.

Academic Significance and Societal Importance of the Research Achievements

本研究により、Ca2+シグナル由来の特定が可能になったことで、細胞内Ca2+シグナルがより精度高く記述できるようになった。これにより、アストロサイトでは従来の予想とは全く異なるCa2+恒常性の維持機構が明らかになった。「たった一種類の伝達物質Ca2+がいかにして多種多様な生命現象を誘導することができるのか」ということは生命科学の本質的な問題である。本研究成果を動物個体、植物、細胞など様々な階層で応用できれば、Ca2+シグナルに込められた生命暗号の一端が解読でき、Ca2+シグナルが関わる生命現象の普遍的な理解に大きく貢献すると期待される。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (22 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (1 results) Journal Article (7 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 6 results,  Open Access: 5 results,  Acknowledgement Compliant: 1 results) Presentation (14 results) (of which Int'l Joint Research: 6 results,  Invited: 14 results)

  • [Int'l Joint Research] University of Dusseldorf(ドイツ)

    • Related Report
      2017 Research-status Report
  • [Journal Article] Dissection of Local Ca<sup>2+</sup> Signals in Cultured Cells by Membrane-targeted Ca<sup>2+</sup> Indicators2019

    • Author(s)
      Bannai Hiroko、Hirose Matsumi、Niwa Fumihiro、Mikoshiba Katsuhiko
    • Journal Title

      Journal of Visualized Experiments

      Volume: 145 Issue: 145

    • DOI

      10.3791/59246

    • Related Report
      2019 Annual Research Report 2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Molecular membrane dynamics: Insights into synaptic function and neuropathological disease2018

    • Author(s)
      Bannai Hiroko
    • Journal Title

      Neuroscience Research

      Volume: 129 Pages: 47-56

    • DOI

      10.1016/j.neures.2017.07.007

    • Related Report
      2018 Research-status Report 2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Astroglial Ca2+ signaling is generated by the coordination of IP3R and store-operated Ca2+ channels2017

    • Author(s)
      Sakuragi S, Niwa F, Oda Y, Mikoshiba K, Bannai H
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 486 Issue: 4 Pages: 879-885

    • DOI

      10.1016/j.bbrc.2017.03.096

    • Related Report
      2017 Research-status Report 2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Basal ryanodine receptor activity suppresses autophagic flux2017

    • Author(s)
      Vervliet T, Pintelon I, Welkenhuyzen K, Bootman MD, Bannai H, Mikoshiba K, Martinet W, Nadif Kasri N, Parys JB, Bultynck G
    • Journal Title

      Biochem Pharmacol.

      Volume: 132 Pages: 133-142

    • DOI

      10.1016/j.bcp.2017.03.011

    • Related Report
      2017 Research-status Report 2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Astrocytic IP3Rs: Contribution to Ca2+ signalling and hippocampal LTP2017

    • Author(s)
      Sherwood MW, Arizono M, Hisatsune C, Bannai H, Ebisui E, Sherwood JL, Panatier A, Oliet SH, Mikoshiba K
    • Journal Title

      Glia

      Volume: 65 Issue: 3 Pages: 502-513

    • DOI

      10.1002/glia.23107

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Astrocytic IP3Rs: Contribution to Ca2+ signalling and hippocampal LTP.2017

    • Author(s)
      Sherwood MW, Arizono M, Hisatsune C, Bannai H, Ebisui E, Sherwood JL, Panatier A, Oliet SH, Mikoshiba K.
    • Journal Title

      Glia

      Volume: 65 Issue: 1 Pages: 502-513

    • DOI

      10.1016/j.bbrc.2016.09.034

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 「2つのシグナル物質の使い分けによる正反対の神経制御ー新たな抑制性シナプス伝達制御メカニズムの発見」2016

    • Author(s)
      丹羽史尋,坂内博子,御子柴克彦
    • Journal Title

      実験医学

      Volume: 34 Pages: 2190-2193

    • Related Report
      2016 Research-status Report
  • [Presentation] Physiology and pathology of brains revealed by single molecule imaging2019

    • Author(s)
      Hiroko Bannai
    • Organizer
      OIST Joint Minisymposium with The 16th International Membrane Research Forum Okinawa
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Physiology and pathology of brains revealed by single molecule imaging2019

    • Author(s)
      Hiroko Bannai
    • Organizer
      17th Chinese Biophysics Congress
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 1分子イメージングで読み解く脳の生理と病理2019

    • Author(s)
      坂内博子
    • Organizer
      和光-精神神経懇話会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] “Physiology and pathology of brains revealed by single molecule imaging”2019

    • Author(s)
      Hiroko Bannai
    • Organizer
      Keynote lecture in OIST Joint Minisymposium with The 16th International Membrane Research Forum Okinawa, 沖縄、2019/3/18-20
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] “Approach to neurodegenerative disease by singularity biology”2018

    • Author(s)
      Hiroko Bannai, Misa Kanatani, Sumihiro Maeda, Matsumi Hirose, Akihiko Takashima, Katsuhiko Mikoshiba
    • Organizer
      第56回日本生物物理学会年会(日本、岡山)
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] "Deciphering the cell individuality of a cell by single molecule analysis of membrane molecule dynamics -What molecular behavior tells us about brain?-2018

    • Author(s)
      Hiroko Bannai
    • Organizer
      MPFI-JST(PRESTO) Joint Workshop on Neuroscience and Single Cell Analysis, Jupitar FL USA, 2018/10/29-30
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] "Approach to role of physiological Tau protein by single molecule analysis of membrane molecule dynamics "2018

    • Author(s)
      Hiroko Bannai, Misa Kanatani, Sumihiro Maeda, Matsumi Hirose, Akihiko Takashima, Katsuhiko Mikoshiba
    • Organizer
      12th International Symposium on Nanomedicine (日本、宇部)2018/12/6-8
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Physiology and pathology of the brain revealed by single molecule dynamics2018

    • Author(s)
      坂内博子、御子柴克彦
    • Organizer
      第123回日本解剖学会総会・全国学術集会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] A new approach to decoding of Ca2+ signals in a single cell2017

    • Author(s)
      Hiroko Bannai, Fumihiro Niwa, Shigeo Sakuragi, Katsuhiko Mikoshiba
    • Organizer
      第54回 日本生物物理学会年会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Molecular mechanism supporting brain functions revealed by single molecule dynamics and subcellular Ca2+ signaling2017

    • Author(s)
      BANNAI Hiroko, Fumihiro Niwa, Shigeo Sakuragi, Katsuhiko Mikoshiba
    • Organizer
      11th International Symposium on Nanomedicine
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Deciphering the property of individual cells by single molecule analysis of membrane molecule dynamics2016

    • Author(s)
      ○坂内博子、丹羽史尋、有薗美沙、御子柴克彦
    • Organizer
      第54回日本生物物理学会年会
    • Place of Presentation
      つくば国際会議場(茨城県・つくば市)
    • Year and Date
      2016-11-26
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 分子のふるまいから読み解く、脳のしくみ2016

    • Author(s)
      ○坂内博子
    • Organizer
      日仏生物学会主催一般公開シンポジウム
    • Place of Presentation
      恵比寿日仏会館(東京・渋谷区)
    • Year and Date
      2016-11-20
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] APPROACH TO THE BRAIN FUNCTION BY IMAGING SINGLE MOLECULE BEHAVIOR IN SINGLE, LIVING CELLS2016

    • Author(s)
      ○Hiroko Bannai
    • Organizer
      International Conference on Single Cell Research 2016
    • Place of Presentation
      東京大学(東京・文京区)
    • Year and Date
      2016-11-17
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 細胞膜分子動態が語る脳のしくみ2016

    • Author(s)
      ○坂内博子
    • Organizer
      第8回生命情報若手の会
    • Place of Presentation
      北海道大学(北海道・札幌市)
    • Year and Date
      2016-10-14
    • Related Report
      2016 Research-status Report
    • Invited

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Published: 2016-04-21   Modified: 2021-02-19  

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