Analysis of dynamics of actomyosin by super-resolution fluorescence imaging
| Project/Area Number |
16K07322
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Osaka University |
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Principal Investigator |
Wazawa Tetsuichi 大阪大学, 産業科学研究所, 特任准教授(常勤) (80359851)
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| Project Period (FY) |
2016-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 超解像イメージング / 蛍光偏光 / タンパク質複合体 / 正則化最尤法 / モータータンパク質 / 細胞骨格 / アクチンフィラメント / ポジティブ型光スイッチング蛍光タンパク質 / アクチン / 偏光 / 蛍光 / 蛍光顕微鏡 / 正則化 / 蛍光変調 / 正則最尤法 / SPoD / 分子モーター / 超精密計測 / ナノバイオ / 生物物理 / 酵素反応 |
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| Outline of Final Research Achievements |
Super-resolution fluorescence imaging is a technique to achieve observation at a higher spatial resolution than conventional optical microscopy. However, long-term time-lapse super-resolution observation at a fast frame rate still remains challenging. In this study we have developed a super-resolution imaging technique by which realistic super-resolved images are obtained with very low photodamage in live cells and intact biomolecules. By the present technique, we successfully achieve long-term super-resolution observation of cytoskeletons such as actin filaments in live cells.
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| Academic Significance and Societal Importance of the Research Achievements |
細胞内には，タンパク質や脂質等で構成される微小なナノ構造複合体が複雑に配置されていて，それらが相互に作用することで機能している．本研究では，従来の超解像顕微鏡では困難であった，生体試料に対する光ダメージを最小にしながら，長時間の観察が可能な超解像イメージング技術を開発した．本研究により，タンパク質複合体が細胞中で機能する現場を高分解能で観察することが可能となり，タンパク質複合体が細胞生理機能においてどのように作用するかを解明することに今後貢献するものと思われる．
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Report
(5 results)
Research Products
(20 results)
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[Journal Article] Highly biocompatible super-resolution fluorescence imaging using the fast photoswitching fluorescent protein Kohinoor and SPoD-ExPAN with Lp-regularized image reconstruction.2018
Author(s)
Wazawa, T., Arai, Y., Kawahara, Y., Takauchi, H., Washio, T., Nagai, T.
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Journal Title
Related Report
Peer Reviewed
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[Presentation] Development of a highly-bright positively reversibly photoswitchable fluorescent protein Kohinoor 2.0 for super-resolution microscopy2019
Author(s)
Wazawa, T., Uto, S., Sugiura, K., Maeda, S., Fujita, K., Washio, T., Nagai, T.
Organizer
Annual Meeting of the Biophysical Society of Japan
Related Report
Int'l Joint Research
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[Presentation] Highly-biocompatible superresolution imaging by SPoD-ExPAN with Lp-regularized image reconstruction2018
Author(s)
Wazawa, T., Arai, Y., Kawahara, Y., Takauchi, H., Washio, T., Nagai, T.
Organizer
日本生物物理学会年会
Related Report
Int'l Joint Research
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