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Analysis of dynamics of actomyosin by super-resolution fluorescence imaging

Research Project

Project/Area Number 16K07322
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biophysics
Research InstitutionOsaka University

Principal Investigator

Wazawa Tetsuichi  大阪大学, 産業科学研究所, 特任准教授(常勤) (80359851)

Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords超解像イメージング / 蛍光偏光 / タンパク質複合体 / 正則化最尤法 / モータータンパク質 / 細胞骨格 / アクチンフィラメント / ポジティブ型光スイッチング蛍光タンパク質 / アクチン / 偏光 / 蛍光 / 蛍光顕微鏡 / 正則化 / 蛍光変調 / 正則最尤法 / SPoD / 分子モーター / 超精密計測 / ナノバイオ / 生物物理 / 酵素反応
Outline of Final Research Achievements

Super-resolution fluorescence imaging is a technique to achieve observation at a higher spatial resolution than conventional optical microscopy. However, long-term time-lapse super-resolution observation at a fast frame rate still remains challenging. In this study we have developed a super-resolution imaging technique by which realistic super-resolved images are obtained with very low photodamage in live cells and intact biomolecules. By the present technique, we successfully achieve long-term super-resolution observation of cytoskeletons such as actin filaments in live cells.

Academic Significance and Societal Importance of the Research Achievements

細胞内には,タンパク質や脂質等で構成される微小なナノ構造複合体が複雑に配置されていて,それらが相互に作用することで機能している.本研究では,従来の超解像顕微鏡では困難であった,生体試料に対する光ダメージを最小にしながら,長時間の観察が可能な超解像イメージング技術を開発した.本研究により,タンパク質複合体が細胞中で機能する現場を高分解能で観察することが可能となり,タンパク質複合体が細胞生理機能においてどのように作用するかを解明することに今後貢献するものと思われる.

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (20 results)

All 2020 2019 2018 2017 2016

All Journal Article (8 results) (of which Peer Reviewed: 7 results,  Open Access: 1 results) Presentation (10 results) (of which Int'l Joint Research: 6 results,  Invited: 1 results) Book (2 results)

  • [Journal Article] Highly-Biocompatible Super-Resolution Imaging: SPoD-OnSPAN2020

    • Author(s)
      Wazawa, T., Washio, T., Nagai, T.
    • Journal Title

      Neuromethods

      Volume: 154 Pages: 229-244

    • DOI

      10.1007/978-1-0716-0532-5_11

    • ISBN
      9781071605318, 9781071605325
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 回転偏光照明蛍光顕微鏡と光スイッチング蛍光タンパク質を用いた超解像イメージング法:SPoD-OnSPAN2019

    • Author(s)
      和沢鉄一,鷲尾隆,永井健治
    • Journal Title

      光アライアンス

      Volume: 30 Pages: 15-19

    • Related Report
      2019 Annual Research Report
  • [Journal Article] SPoD-Net: Fast recovery of microscopic images using learned ISTA2019

    • Author(s)
      Hara, S., Chen, W., Washio, T., Wazawa, T., Nagai, T.
    • Journal Title

      PMLR

      Volume: 101 Pages: 694-709

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Acid-Tolerant Reversibly Switchable Green Fluorescent Protein for Super-resolution Imaging under Acidic Conditions2019

    • Author(s)
      Shinoda Hajime、Lu Kai、Nakashima Ryosuke、Wazawa Tetsuichi、Noguchi Kosuke、Matsuda Tomoki、Nagai Takeharu
    • Journal Title

      Cell Chemical Biology

      Volume: 26 Issue: 10 Pages: 1469-1479.e6

    • DOI

      10.1016/j.chembiol.2019.07.012

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Highly biocompatible super-resolution fluorescence imaging using the fast photoswitching fluorescent protein Kohinoor and SPoD-ExPAN with Lp-regularized image reconstruction2018

    • Author(s)
      Tetsuichi Wazawa, Yoshiyuki Arai, Yoshinobu Kawahara, Hiroki Takauchi, Takashi Washio, Takeharu Nagai
    • Journal Title

      Microscopy

      Volume: 67 Issue: 2 Pages: 89-98

    • DOI

      10.1093/jmicro/dfy004

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Highly biocompatible super-resolution fluorescence imaging using the fast photoswitching fluorescent protein Kohinoor and SPoD-ExPAN with Lp-regularized image reconstruction.2018

    • Author(s)
      Wazawa, T., Arai, Y., Kawahara, Y., Takauchi, H., Washio, T., Nagai, T.
    • Journal Title

      Microscopy

      Volume: 67

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] SPoD-ExPAN 超解像イメージング2017

    • Author(s)
      和沢鉄一,新井由之,永井健治
    • Journal Title

      顕微鏡

      Volume: 52 Pages: 77-81

    • NAID

      130007689235

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Strong Dependence of Hydration State of F Actin on the Bound Mg2+/Ca2+ Ions2016

    • Author(s)
      Makoto Suzuki, Asato Imao, George Mogami, Ryotaro Chishima, Takahiro Watanabe, Takaya Yamaguchi, Nobuyuki Morimoto, and Tetsuichi Wazawa
    • Journal Title

      J. Phys. Chem. B

      Volume: 120 Issue: 28 Pages: 6917-6928

    • DOI

      10.1021/acs.jpcb.6b02584

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] Development of a highly-bright positively reversibly photoswitchable fluorescent protein Kohinoor 2.0 for super-resolution microscopy2019

    • Author(s)
      Wazawa, T., Uto, S., Sugiura, K., Maeda, S., Fujita, K., Washio, T., Nagai, T.
    • Organizer
      Annual Meeting of the Biophysical Society of Japan
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 回転偏光照明,ポジティブ光スイッチング蛍光タンパク質,そして Lp 正則化画像再構成を用いた超解像イメージング: SPoD-OnSPAN2019

    • Author(s)
      和沢鉄一,杉浦一徳,鷲尾 隆,永井健治.
    • Organizer
      第44回レーザー顕微鏡研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Highly-biocompatible superresolution imaging by SPoD-ExPAN with Lp-regularized image reconstruction2018

    • Author(s)
      Wazawa, T., Arai, Y., Kawahara, Y., Takauchi, H., Washio, T., Nagai, T.
    • Organizer
      日本生物物理学会年会
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Genetically encoded photoswitchable indicators towards super-resolution calcium imaging.2018

    • Author(s)
      Lu, K., Matsuda, T., Wazawa, T., Fujiwara, S., Nagai, T.
    • Organizer
      日本生物物理学会年会
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Biocompatible super-resolution imaging by SPoD-ExPAN and fast photoswitchable fluorescent protein2018

    • Author(s)
      Wazawa, T.
    • Organizer
      First UK/Japan Super-resolution Bioimaging Meeting
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Recursive BC を用いた超解像顕微鏡の画像推定手法2018

    • Author(s)
      木戸 俊輔, 鷲尾 隆, 和沢 鉄一, 永井 健治
    • Organizer
      2018年度人工知能学会全国大会(第32回)
    • Related Report
      2018 Research-status Report
  • [Presentation] 遺伝子工学的に開発した蛍光プローブによる細胞生理機能超解像イメージング2017

    • Author(s)
      和沢鉄一,新井由之,河原吉伸,中野雅裕,鷲尾隆,永井健治
    • Organizer
      第31回日本人工知能学会全国大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Superresolution imaging of live cells by fast photoswitching fluorescent protein and improved SPoD-ExPAN microscopy2016

    • Author(s)
      Tetsuichi Wazawa, Yoshiyuki Arai, Tomoki Matsuda, Hiroki Takauchi, Yoshinobu Kawahara, Takashi Washio, Takeharu Nagai
    • Organizer
      Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      つくば国際会議場
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 新規光スイッチング蛍光タンパク質による超解像イメージング2016

    • Author(s)
      和沢鉄一,新井由之
    • Organizer
      第140回生命機能研究科研究交流会
    • Place of Presentation
      大阪大学大学院生命機能研究科
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] Superresolution imaging of live cells by fast photoswitching fluorescent protein and improved SPoD-ExPAN microscopy2016

    • Author(s)
      Tetsuichi Wazawa, Yoshiyuki Arai, Tomoki Matsuda, Hiroki Takauchi, Yoshinobu Kawahara, Takashi Washio, and Takeharu Nagai
    • Organizer
      The 20th SANKEN International Symposium
    • Place of Presentation
      Knowledge Capital Congres Convention Center
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Book] 実験医学増刊:『生きてるものは全部観る!イメージングの選び方・使い方100+』(42節:高生体適合性SPoD-ExPANイメージング)2018

    • Author(s)
      和沢鉄一,永井健治
    • Total Pages
      235
    • Publisher
      羊土社
    • Related Report
      2018 Research-status Report
  • [Book] 初めてでもできる!超解像イメージング:2.6節 RESOLFT とSPoD の原理と変法2016

    • Author(s)
      和沢鉄一,永井健治
    • Publisher
      羊土社
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2021-02-19  

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