| Project/Area Number |
16K07334
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Tohoku University |
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Principal Investigator |
HARUTA Nami 東北大学, 生命科学研究科, 助教 (70381671)
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| Research Collaborator |
SUGIMOTO Asako
UCHIYAMA Chihiro
NAKAJO Momoe
SAITO Yuki
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | γ-チューブリン複合体 / C.elegans / 微小管 / c. elegans / ガンマチューブリン / 中心体 / 細胞骨格 |
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| Outline of Final Research Achievements |
The γ-tubulin complex (γTuC) is a widely conserved microtubule (MT) nucleator, which serves as a template for MTs. To understand the molecular mechanism of the assembly and recruitment of γTuC, we characterized several components of γTuC; MOZART1, GTAP-1 and -2. We demonstrated that the interaction between MOZART1 and N-terminus of GIP-1 is essential for the centrosomal recruitment of γTuC, while GTAP-1 and GTAP-2 are not essential but required for efficient recruitment of γTuC. Moreover, GTAP-1 plays a crucial role in germline through controlling the localization of γ-tubulin onto gonad membrane, although GTAP-2 is dispensable. These data strongly indicated that the components of γTuC are likely changed, depending on the cell cycles and types.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究を通じて、γ-チューブリン複合体（γTuC）構成因子間で明確な役割の差があり、微小管の形成する場所によって必要性も異なることがわかった。これは、細胞の種類と時期特異的にγTuC複合体の構成とその制御機構が異なることを強く示唆する結果であり、線虫のみならず、動物における組織特異的なγTuCによる微小管形成制御の分子基盤の理解にもつながる成果である。
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