| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
TIF6 was isolated as a gene related to the ubiquitin-proteasome system (UPS) in S. cerevisiae. TIF6 (translation initiation factor 6) is an essential gene, and encodes one of the chaperone proteins involved in the biogenesis of the 60S ribosome. DAmP (decreased abundance by mRNA perturbation) mutant cells of Tif6 showed three phenotypes, all of which indicate the relation between Tif6 and the UPS. 1) tif6-DAmP cells are sensitive to the amino acid analogs. 2) The model substrates of the 26S proteasome are not efficiently degraded in the tif6-DAmP mutant. 3) Double mutations of TIF6 and the proteasome genes cause synthetic growth defects. Tif6 is a highly conserved among eukaryotes, and its human ortholog is eIF6 (>70% identity). Thus, we next investigated the relation between eIF6 and the UPS. Knockdown of eIF6 led to retardation of the assembly of proteasomes, and yeast two-hybrid assays indicated that eIF6 interacts with some proteasome subunits.
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