| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Cells are consistently subjected to wounding by physical or chemical damages from the external environment. However, the cells have an ability to repair the wounded cell membrane. In addition, methods for introducing extracellular substances into cells such as electroporation rely on cellular wound repair. In the present study, we newly invented a laserporation method to make a pore in the cell membrane. By using this method, we examined the mechanism of cell membrane repair. We characterized the dynamics of wound pores opening and closing by live imaging of fluorescent cell membrane proteins, influx of fluorescent dye, and calcium ion imaging. We also found that annexin C1 immediately accumulated at the wound site depending on the external calcium ion. These results will contribute not only to basic researches but also to the therapy for membrane repair-related diseases.
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