Functional analysis of a novel extracellular PCP component shiome
Project/Area Number |
16K07363
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Developmental biology
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Research Institution | Chiba University |
Principal Investigator |
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Project Period (FY) |
2016-04-01 – 2019-03-31
|
Project Status |
Completed (Fiscal Year 2018)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 平面内細胞極性 / 発生・分化 / シグナル伝達 / 平面細胞極性 |
Outline of Final Research Achievements |
The function of the novel gene shiome, which controls planar cell polarity (PCP) formed on the epithelial tissues, was investigated using Drosophila. As a result, it was revealed that the PCP of the adult wing was disrupted by overexpression of shiome, larvae of shiome mutants showed the disruption of PCP on the epidermal cuticle, and shiome was required for molting from the first instar to the second instar larvae. Furthermore, biochemical studies showed that Shiome was secreted protein. Based on the above results, we concluded that Shiome is a multifunctional secretory protein required for establishment of PCP and molting in Drosophila.
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Academic Significance and Societal Importance of the Research Achievements |
本研究により、多細胞からなるシートから動物の形が正しく作られる仕組みの一端を明らかにすることができた。また、本研究で用いたショウジョウバエは、遺伝子やタンパク質のレベルでヒトと共通した点も多く、本研究で明らかにしたことは、多細胞動物の共通の仕組みである可能性がある。一方で、本研究で明らかにした形作りの遺伝子が、ショウジョウバエを含む節足動物と一部の動物に特有の脱皮という一見すると関係のなさそうな別の生命現象にも必要である点は動物の進化と多様性を考える上で興味深い。
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Report
(4 results)
Research Products
(20 results)