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Mechanisms for the establishment of the zic1 expression boundary in the teleost trunk

Research Project

Project/Area Number 16K07364
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Developmental biology
Research InstitutionThe University of Tokyo

Principal Investigator

Shimada Atsuko  東京大学, 大学院理学系研究科(理学部), 客員共同研究員 (20376552)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords遺伝子発現境界 / 体節 / zic1 / メダカ / medaka / somite
Outline of Final Research Achievements

Establishment of gene expression boundary is crucial for creating elaborate morphology during development. We examined the establishment of zic1/zic4-expression boundary demarcating dorsoventral boundary of the entire trunk of medaka fish (Oryzias latipes), and identified a subgroup of dermomyotomal cells, called horizontal boundary cells (HBCs) as crucial players for the boundary formation. Embryological and genetic analyses demonstrated that HBCs play crucial roles in the two major events of the process, i.e. refinement and maintenance. In the refinement, HBCs could serve as a chemical barrier against Wnts from the neural tube by expressing Hhip. At later stages, HBCs participate in the maintenance of the boundary by differentiating into the horizontal myoseptum physically inhibiting cell mixing across the boundary. These findings reveal the mechanisms underlying the dorsoventral boundary in the teleost trunk by specialized boundary cells.

Academic Significance and Societal Importance of the Research Achievements

本研究では、メダカ体節の背腹中心部に出現する新規細胞群Boundary cellsを同定し、この細胞群が背腹を分断しつつzic1発現境界を形成することで体幹部を背腹のコンパートメントに分けることを示した。従来わかっていたコンパートメント形成メカニズムは、区画間の細胞同士の相互作用に着目したものであり(接着性の違い等)、本研究が提唱する特殊な細胞による遺伝子発現境界形成は新規なコンパートメント形成メカニズムである。このようなメカニズムは、大型のからだを有する脊椎動物のかたちをロバストに維持するための普遍的な戦略である可能性があり学術的に意義がある。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (6 results)

All 2019 2017 2016 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (4 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Remarks (1 results)

  • [Journal Article] Horizontal Boundary Cells, a Special Group of Somitic Cells, Play Crucial Roles in the Formation of Dorsoventral Compartments in Teleost Somite2019

    • Author(s)
      Kota Abe,Atsuko Shimada,Sayaka Tayama,Hotaka Nishikawa,Takuya Kaneko, Sachiko Tsuda,Akari Karaiwa,Takaaki Matsui,Tohru Ishitani,Hiroyuki Takeda
    • Journal Title

      Cell Reports

      Volume: 27 Issue: 3 Pages: 928-939

    • DOI

      10.1016/j.celrep.2019.03.068

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Cellular and molecular mechanisms for the establishment of the dorsal and ventral compartments in the teleost somite2017

    • Author(s)
      Kota Abe, Atsuko Shimda, Hiroyuki Takeda
    • Organizer
      50th annual meeting of the Japanese Society of Developmental Biologists
    • Related Report
      2017 Research-status Report
  • [Presentation] Cellular and molecular mechanisms for the establishment of the lifelong dorsoventral compartment boundary in the teleost trunk2017

    • Author(s)
      Kota Abe, Atsuko Shimda, Hiroyuki Takeda
    • Organizer
      18th international congress of developmental biology
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Lineage analysis of osteoblasts in fins and scales of the medaka2016

    • Author(s)
      Sayaka Tayama, Atsuko Shimada, Hiroyuki Takeda
    • Organizer
      第87回日本動物学会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県宜野湾市)
    • Year and Date
      2016-11-17
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Development of the zic-expressing dorsal compartment in fish and its implications for evolution of the vertebrate trunk morphology2016

    • Author(s)
      Atsuko Shimada, Kota Abe, Hiroyuki Takeda
    • Organizer
      第22回国際動物会議
    • Place of Presentation
      沖縄科学技術大学院大学(沖縄県国頭郡恩納村)
    • Year and Date
      2016-11-14
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Remarks] 東京大学動物学発生学研究室ホームページ

    • URL

      http://www.biol.s.u-tokyo.ac.jp/users/hassei/

    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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