Study on regulation of FGF signaling during planarian regeneration

• Project/Area Number: 16K07376
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Developmental biology
• Research Institution: University of Hyogo
• Principal Investigator: Umesono Yoshihiko 兵庫県立大学, 生命理学研究科, 教授 (20391881)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: FGFシグナル / 再生 / プラナリア

Outline of Final Research Achievements

Planarians exhibit extraordinarily high regenerative ability based on somatic pluripotent stem cells. Previous studies suggest that fibroblast growth factor (FGF) signaling plays a crucial role in the regulation of head tissue differentiation during planarian regeneration. In this study, we have established an assay system using Xenopus laevis embryos and examined whether planarian FGF (DjFGF) can bind Ig domains of planarian FGF receptor-related protein. This assay provides evidence of positive interaction between DjFGF and Ig domains of a certain planarian FGF receptor-related protein in vivo.

Academic Significance and Societal Importance of the Research Achievements

今回の研究成果により、プラナリアは、nou-darakeを代表とする偽FGF受容体遺伝子をそのゲノムに複数個獲得することによって、FGF活性調節の多様性を生み出すという脊椎動物とは異なる非常にユニークなゲノム戦略を進化の過程で獲得したことが考えられた。

Research Products

• [Journal Article] Usefulness of multiple chalk-based food colorings for inducing better gene silencing by feeding RNA interference in planarians (2018)
  • Author(s): Hattori, M., Miyamoto, M., Hosoda, K., Umesono Y.
  • Journal Title: Development Growth & Differentiation Volume: 60 Issue: 1 Pages: 76-81
  • DOI: 10.1111/dgd.12413
  • Peer Reviewed
• [Journal Article] A transgenic reporter under control of an es1 promoter/enhancer marks wound epidermis and apical epithelial cap during tail regeneration in Xenopus laevis tadpole (2018)
  • Author(s): Sato, K., Umesono, Y., Mochii, M.
  • Journal Title: Developmental Biology Volume: 433 Issue: 2 Pages: 404-415
  • DOI: 10.1016/j.ydbio.2017.08.012
  • Peer Reviewed
• [Presentation] プラナリアの前後軸再生機構の解明 (2018)
  • Author(s): 梅園良彦
  • Organizer: 再生学異分野融合研究会
  • Invited
• [Presentation] Molecular logic for robust organ positioning according to the anterior-posterior axis size during planarian regeneration (2017)
  • Author(s): 細田和孝、梅園良彦
  • Organizer: Joint Meeting of the German and Japanese Societies of Developmental Biologists
  • Place of Presentation: キール（ドイツ）
  • Year and Date: 2017-03-15
  • Int'l Joint Research / Invited
• [Presentation] プラナリアの前後軸に沿ったからだのプロポーション形成を司る分子基盤 (2017)
  • Author(s): 梅園良彦
  • Organizer: 生命科学系学会合同年次大会 （ConBio2017)
  • Invited
• [Presentation] Molecular logic of planarian regeneration along the head-to-tail axis (2016)
  • Author(s): 梅園良彦
  • Organizer: 第22回国際動物学会 第87回日本動物学会合同大会
  • Place of Presentation: OIST、沖縄県恩納村
  • Year and Date: 2016-11-14
  • Int'l Joint Research
• [Presentation] プラナリアにおける脳を介さない摂食行動の解析 (2016)
  • Author(s): 宮本真衣、服部美希、細田和孝、梅園良彦
  • Organizer: 日本発生生物学会 秋期シンポジウム2016
  • Place of Presentation: 三島市民文化会館、静岡県三島市
  • Year and Date: 2016-10-19